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AbstractFull Text

Exogenous gene stably integrated into the host genome, and stably inherited to the offspring, producing transgenic animals is a basic requirement. Analysis of transgenic animals for inheritance and expression stability of transgene is an important part of establishing a transgenic animal strain....

Author(s)
Xing XiangYang; Yu ShuZhen; Zhu HuiBin; Pan DengKe; Yang Chun
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 6, pp 1084-1092
AbstractFull Text

Efficient expression of human complement regulatory gene human cluster of differentiation 55 (hCD55) is particularly important in xenotransplantation. Human CD55 gene was knocked-in porcine Rosa26 (pRosa26) locus via CRISPR/Cas9 on the basis of GGTA1 knockout (α-1,3-galactosyltransferase knockout,...

Author(s)
Zhang Chao; Shi NingNing; Gao JingBo; Du MinJie; Zhang YiFu; Wang Yu; Zhu HuiBin; Pan DengKe; Liu Xia
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 6, pp 1074-1083
AbstractFull Text

N-acetylneuraminic acid (Neu5 Gc) is an important non-galactose antigen which can cause xenograft rejection, and it is catalyzed synthesis by cytidine monophospho-N-acetylneuraminic acid hydroxylase (CMAH). In this study, the CMAH gene knockout Bama mini pigs (CMAH-/-) were generated by CRISPR/Cas9 ...

Author(s)
Huang LinHua; Li XiRui; Long Chuan; Shi NingNing; Feng Chong; Du MinJie; Zhu HuiBin; Pan DengKe; Sun XiuZhu
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 6, pp 1064-1073
AbstractFull Text

Bacillus strains are widely used as animal probiotics. Cellulase produced by Bacillus strains could be used to increase efficiency of feed utilization and improve the intestinal microflora. The aim of this study was to screen the Bacillus strains with high celluloytic activities from the...

Author(s)
Liu GuoHong; Liu Bo; Che JianMei; Wang JiePing; Chen QianQian; Yao YunFa; Liu QinYing
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 6, pp 1043-1055
AbstractFull Text

Slow skeletal muscle troponin I 1 (TNNI1) located in slow-twitch myofibers that provides a calcium-sensitive switch for the contraction of striated muscle and plays an important role in muscle growth. The aim of this study was to clone the cDNA full length of TNNI1 gene of Gaoyou duck (Anas...

Author(s)
Ji GaiGe; Shu JingTing; Shan YanJu; Zhang Ming; Tu YunJie; Ju XiaoJun; Liu YiFan
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 6, pp 970-977
AbstractFull Text

As a key enzyme involved in the formation and regulation of melanin, tyrosinase (TYR) has an important role in animal hair color phenotype. Tyrosinase dysfunction can block the pathway of melanin formation. The mutation of tyrosinase causes white phenotype of skin, hair, and feathers....

Author(s)
Liu XiaoHui; Zhou RongYan; Zhang ChuanSheng; Peng YongDong; Li XiangLong
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 6, pp 959-969
AbstractFull Text

The miR-124a-3p has a potential interaction relationship with the acetyl-CoA acyltransferase 2 gene (ACAA2) in chicken (Gallus gallus). The research around biological function of chicken miR-124a-3p and its targeting effect on ACAA2 gene have not been reported. This study analyzed the potential...

Author(s)
Fu ShouYi; Chen YaDi; Wang Jie; Yan FengBin; Sun GuiRong; Li ZhuanJian; Han RuiLi; Kang XiangTao; Li GuoXi
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 6, pp 949-958
AbstractFull Text

Clinical mastitis is a kind of the most serious disease with high incidence in dairy industry, and has been plagued in the development of dairy industry. It has significant effects on milk performance and lifetime which will cause huge economic losses in milk production process. It's very important ...

Author(s)
Wang MengQi; Ni Wei; Tang Cheng; Guo JiaHe; Zhang HuiMin; Li MingXun; Yang ZhangPing; Mao YongJiang
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 5, pp 811-818
AbstractFull Text

Litter size is one of the most important economic traits in sheep (Ovis aries) which is controlled by minor genes. While genes that have been found to be related to litter size are numbered, and it is still a hotspot to mine them. To investigate the tissue expression level and polymorphisms of...

Author(s)
Zhou Mei; Cao XiaoHan; He XiaoYun; Sun Qing; Di Ran; Hu WenPing; Wang XiangYu; Zhang XiaoSheng; Zhang JinLong; Liu QiuYue; Chu MingXing
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 5, pp 801-810
AbstractFull Text

The formation of skeletal muscle affects the growth and development of animals. Through screening SNPs of MyoD1 gene promoter in different breeds of cattle (Bos taurus), and studying its effect on growth body size traits and promoter function, to further provide research basis for the molecular...

Author(s)
Zhou Di; Xu HouQiang; Chen Wei; Zhao JiaFu; Duan ZhiQiang
Publisher
Journal of Agricultural Biotechnology, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2018, 26, 5, pp 743-755

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