An old problem revisited: biological control of toadflaxes
Native to Europe, toadflaxes were introduced to the USA and Canada over 100 years ago as ornamental plants. They now occur over much of temperate North America and are declared noxious in eight US states. CABI identifies specific natural enemies that can be introduced into North America as biological control agents to reduce the vigour, density and spread of this invasive plant.
So, what’s the problem
Yellow toadflax (Linaria vulgaris) and Dalmatian toadflax (L. dalmatica) are short-lived forbs that spread both by seeds and vegetatively. Both perennial plant species originate from central Europe and were introduced to North America in the 17th and 19th centuries, respectively, as ornamental plants. They now occur in much of temperate North America. The plants grow best on disturbed soils, grasslands, roadsides and areas after a wildfire has broken out. Dalmatian toadflax in particular is mainly a problem weed in dry, rocky or gravelly habitats, whilst yellow toadflax can be a problem when growing crops.
So far, six insect species have been released in North America to control the plants as biocontrol agents; three others have been accidentally introduced. Our studies show that cryptic speciation (morphologically similar individuals that cannot interbreed) is occurring together with a high degree of host-plant specialization in several biocontrol agents associated with the genus Linaria in southeastern Europe, meaning that closely related but different insects that can’t breed with each other specialize either on yellow toadflax or Dalmatian toadflax. This is the case with shoot-galling weevils in the genus Rhinusa and with shoot-mining weevils in the genus Mecinus. A combined morphological, molecular and biological study has shown that the weevil species presently named M. janthinus is actually composed of two species, M. janthiniformis sp. n., which attacks Dalmatian toadflax and M. janthinus, which attacks yellow toadflax.
Based on work conducted at CABI’s centre in Switzerland, both species were introduced to North America in the 1990s. Impact of M. janthiniformis reported shortly after its introduction showed significant reductions of Dalmatian toadflax at several release sites. M. janthinus is also now increasing in density and showing promise in both the USA and Canada.
More biocontrols are needed however if these two weeds are to be successfully tackled.
What is this project doing?
As a result, several other European insects have been investigated by CABI since 2000. Our scientist, Ivo Toševski, has been working on this in Serbia, investigating the potential use of insects that can control Dalmatian and especially yellow toadflax.
Following our research, the shoot-galling weevil Rhinusa pilosa, which specializes on yellow toadflax, was released in Alberta and British Columbia, Canada in 2014. It established and produced galls at all release sites and also overwintered successfully, but has not reached outbreak densities yet. In 2018, its release was also approved in the USA, and first field releases are planned for 2019 in Montana. Testing with its sister species, R. rara, on Dalmatian toadflax has been completed and a petition to Canadian and US authorities will be submitted soon.
Safety testing is also near to completion for two additional stem-mining weevils in the genus Mecinus, ie M. heydeni for yellow toadflax, and M. laeviceps for Dalmatian toadflax. No-choice tests (where weevils are confined to either test or target plants) showed that egg laying and larval development of both species are restricted to species in the Linaria genus and a few native North American species in the genera Nuttallanthus and Sairocarpus. For a third species, M. peterharrisi on Dalmatian toadflax, testing is ongoing. Apart from Linaria, development only occurred on Nuttallanthus canadensis so far and only under no-choice conditions. A test with all three Mecinus species showed that the weevils cannot survive when only provided with a test species for aestivation and overwintering, confirming their restricted host range. None of the adults of the three species overwinter in the stems of toadflax itself, but spend the winter in leaf litter which should increase their survival and help build up their populations. M. peterharrisi occurs at higher elevations in Europe and should therefore be particularly suited for more northern regions in North America.
Dr Andrew Norton, Colorado State University, USA
Dr Sharlene Sing, US Forest Service, Rocky Mountain Research Station
Dr Rosemarie De Clerck-Floate, Agriculture and Agri-Food Canada
Dr Brent Emerson, Island Ecology and Evolution Research Group, Spain
Dr Roberto Caldara, Milan, Italy