After observing limited participation of women in the programme intially, some Plantwise implementing countries have taken different measures to address the problem. It will be useful to understand how such measures helped improve women’s participation, as well as, the overall constraints women faced to participate and benefit from the programme interventions. This understanding would be useful to inform the development of the next phase of the Plantwise programme, to enable it to contribute to more gender equitable outcomes. The overall objective this assessment is to examine how gender is mainstreamed in the Plantwise programme by indentifying strengths and limitations and drawing out lessons that will inform the development of the next phase of the programme.