The autoagglutination test and direct and indirect Coombs, tests are used in the diagnosis of immune-mediated haemolytic anaemia (IMHA). The autoagglutination test is a test that the veterinary practitioner can easily implement in the clinic, but is only useful if autoagglutination is detected in...

Between June 2005 and December 2006 522 dogs were included in this study (group 1: 258 sick dogs suspicious for CGA, group 2: 264 clinically healthy dogs). Suspicious for CGA were dogs with clinical signs such as lethargy, fever, inappetence, pale mucous membranes, hemorrhage, joint pain,...

The aim of the present case report is to describe the outcome, hematological and biochemical changes of treatment of idiopathic thrombocytopenia in a Terrier dog using human intravenous immunglobulin. The complete blood count, serum biochemistry, indirect immunofluorescence antibody and direct ...

Objectives: Canine allo- or autoantibodies are clinically important, but attachment of these immunoglobulin G (IgG) antibodies does not produce observable haemagglutination. Antibody to canine globulins is required to demonstrate sensitisation of red blood cells. Commercial reagents are available,...

Ultraviolet (UV)-irradiated whole dog blood prevented the start of proliferative responses in allogeneic mixed lymphocyte reactions (MLR). Of 18 dogs (10 male and 8 female Beagles aged 1 to 6 years) 6 were given 4 weekly i.v. transfusions of UV-irradiated allogeneic donor whole blood, peripheral...

The ability of a cell wall protein from Staphylococcus aureus, Protein A, (SpA) to bind the Fc region of most mammalian IgG molecules is used as the basis for a quantitative assay for erythrocyte (RBC) bound antibodies. Test serum is incubated in a suspension of normal RBC's. The cells are then...

Diagnostic techniques reviewed are tests to diagnose systemic immune-mediated skin diseases, histopathological evaluation of skin, direct immunofluorescence testing, immunoperoxidase testing, blood tests, haematology and blood chemistry, antinuclear antibody test, lupus erythematosus cell test, ...

Blood samples from 34 dogs with autoimmune haemolytic anaemia were studied using a variety of serodiagnostic tests. Enzyme enhancement of red cells was found to be necessary for an unequivocal laboratory diagnosis because other techniques, the direct and the indirect Coombs' tests, often gave...

Thirty-five cases of B. gibsoni infection and 11 of B. canis infection were diagnosed and treated in dogs at Kadena Air Base in Okinawa, Japan, between April 1979 and February 1980. Diagnosis was made by demonstrating the organisms in blood smears and by serological examination, using an indirect...

Haemolytic anaemia was induced in 41 Beagles by different doses of an unspecified drug, or by salbutemol sulphate. Anti-erythrocyte antibodies were detected by direct and indirect Coombs' tests after the erythrocytes had been tested with papain to uncover the reactive sites. The antinuclear...