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VetMed Resource

Veterinary information to support practice, based on evidence and continuing education

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Abstract

The progress of the latest laboratory detection methods for IBRV from the virus isolation and identification of the disease, serology and molecular biology, were reviewed in order to provide reference for the diagnosis and prevention and control of the IBR. Bovine infectious rhinotracheitis (IBR)...

Author(s)
Zhuang JinQiu; Mei JianGuo; Liu JiShan; Zhang Ying; Mo Ling; Yao ChunYang
Publisher
Lanzhou Institute of Animal Husbandry and Veterinary, CAAS, Lanzhou, China
Citation
China Herbivore Science, 2018, 38, 5, pp 40-43, 54
Abstract

1,121 Serum samples from swine, cattle and sheep immunized with foot-and-mouth disease vaccine provided by the animal disease prevention and control centers of various cities in Guangxi, China were tested by LB-ELISA kits and SPC-ELISA kits, to compare the effects of the 2 ELISA kits on detecting...

Author(s)
Lv XiaoLi; Yao XiaoYun; He QiSong; Feng ShuPing; Ma Jun; Qin YuYang; Li XueMei; Xiong Yi; Yan JianHua
Publisher
Ministry of Agriculture, Animal Health and Epidemiology Center of China, QuidDoa, China
Citation
China Animal Health Inspection, 2016, 33, 3, pp 71-73
Abstract

707 Nasal swab samples were collected from apparently healthy cattle and sheep in Shandong province of China in 2014. RNA was extracted from these samples and detected by RT-PCR using primers specific to influenza viruses. RT-PCR products of the positive samples were sequenced. The border disease...

Author(s)
Peng Cheng; Wang SuChun; Zhuang QingYe; Hou GuangYu; Huang Juan; Shan Hu; Chen JiMing
Publisher
Ministry of Agriculture, Animal Health and Epidemiology Center of China, QuidDoa, China
Citation
China Animal Health Inspection, 2015, 32, 3, pp 1-3
Abstract

According to the conservative sequence of the 5'-untranslated region (5'-UTR) of bovine viral diarrhoea virus (BVDV) genome in GenBank, 2 pairs of primers were designed for detecting BVDV I and BVDV II and an RT-PCR was developed to spontaneously detect BVDVI and II in one reaction system after...

Author(s)
Xiong Hao; Ji XinCheng; Wang KeDong; Shi Qian; Ran DuoLiang; Peng WuLi; Yu XueHui
Publisher
Ministry of Agriculture, Animal Health and Epidemiology Center of China, QuidDoa, China
Citation
China Animal Health Inspection, 2013, 30, 4, pp 39-42
Abstract

In order to meet the requirements for detecting and monitoring SAT II foot-and-month disease virus (FMDV), the nucleotide of the specific antigen epitopes was synthesized and expressed in vitro. The expressed fusion protein pGEX-VP1-VP3 was digested on column to prepare the VP1-VP3 protein which...

Author(s)
Deng JunHua; Wu ShaoQiang; Lin XiangMei
Publisher
Ministry of Agriculture, Animal Health and Epidemiology Center of China, QuidDoa, China
Citation
Chinese Journal of Animal Health Inspection, 2011, 28, 2, pp 34-36, 46
Abstract

A quantifiable in situ immune fluorescent assay (IFA) was developed to measure bluetongue virus (BTV) binding to mammalian cells. The utility of the assay was demonstrated with both Chinese hamster ovary (CHO) and bovine pulmonary artery endothelial (CPAE) cells. Since heparin sulfate (HS) has been ...

Author(s)
Mecham, J. O.; McHolland, L. E.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Journal of Virological Methods, 2010, 165, 1, pp 112-115
Abstract

An one-step, single tube, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of peste des petits ruminant virus (PPRV). A set of primers were designed based on the N gene reference sequences of the PPRV. The assay was optimized to amplify...

Author(s)
Li Lin; Wu XiaoDong; Bao JingYue; Li JinMing; Wang JunWei; Wang ZhiLiang
Publisher
Ministry of Agriculture, Animal Health and Epidemiology Center of China, QuidDoa, China
Citation
Chinese Journal of Animal Health Inspection, 2010, 27, 4, pp 32-34&41
Abstract

The infectious bovine rhinotracheitis virus (IBRV) antigen from the virus culture without serum was coated on nitrocellulose (NC) membrane, then the test serum was added to NC membrane, and the color reaction was done with nano-colloidal gold-conjugated goat-anti-bovine IgG. The nano-colloidal gold ...

Author(s)
Wang WuJun; Xu ShuFei; Kong FanDe; Tang TaiShan; Huang YiFan; Bai QuanYang; Zheng Teng
Publisher
Chinese Veterinary Science, Lanzhou, China
Citation
Chinese Veterinary Science / Zhongguo Shouyi Kexue, 2012, 42, 8, pp 831-836
Abstract

An indirect ELISA was developed to detect antibodies against IBRV (infectious bovine rhinotracheitis virus) using recombinant gD protein from IBRV. A pET30a-gD expression plasmid was constructed, and IBRV recombinant gD protein was expressed in Escherichia coli. Recombinant proteins were detected...

Author(s)
Dong HuaXing; Hou XiLin; Xie JinXin; Zhang PeiXin; Liu Qiang; Liu Peng; Wang YanTao
Publisher
Guoji Shudian (Institute of Scientific and Technological Information of China, China Publications Centre), Beijing, China
Citation
Chinese Journal of Veterinary Medicine, 2011, 47, 2, pp 3-5
Abstract

A pair of primers were designed for polymerase chain reaction (PCR) according to the glycoprotein B (gB) gene of infectious bovine herpesvirus (IBHV). The PCR assay was optimized and a specific fragment of 362 bp was obtained. The optimal annealing temperature was 58-64°C and the optimal reaction...

Author(s)
Xu ShuFei; Kong FanDe; Zhou BinHua
Publisher
Editorial Department of Chinese Journal of Animal Quarantine, Shandong, China
Citation
Chinese Journal of Animal Quarantine, 2006, 23, 11, pp 26-28

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