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Abstract

Surviving epithelium from tongues of cattle that were vaccinated against F. & M. disease and from others that had recovered from the infection was used successfully in the tissue-culture titration method described by Brooksby & Wardle [V.B. 24, 2699]. In comparative titrations in cattle and in...

Author(s)
GILLESPIE, J. H.; FRENKEL, H. S.
Citation
American Journal of Veterinary Research, 1955, 16, pp 505-510
Abstract

F. described a method for the cultivation of the virus of F. & M. disease in surviving explanted epithelial tissue taken from the tongues of adult susceptible cattle. The titre of the virus usually ranges from 10-5to 10-8. The average quantity of virus suspension obtained from the 4 to 8 g. of...

Author(s)
FRENKEL, H. S.
Citation
American Journal of Veterinary Research, 1950, 11, pp 371-373
Abstract

The authors report the successful use of epithelium from the non-papillated portions of the bovine rumen for the in-vitro cultivation of F. & M. disease virus. The method used is the same as that described by Frenkel see preceding abst.] for cultivation of the virus in bovine tongue epithelium. In...

Author(s)
FRENKEL, H. S.; FREDERIKS, H. H. J.
Publisher
Lond.,
Citation
Nature, 1949, 164, pp 235-236
Abstract

A method and apparatus are described by which the epithelium of the tongue of cattle may be stripped off and used for tissue culture of the virus. The method has been used with success for the growth of the virus in sufficiently large quantities to be used for the preparation of adsórbate vaccine...

Author(s)
FRENKEL, H. S.
Publisher
Office International des Epizooties, Paris, France
Citation
Bulletin de l'Office International des Epizooties, 1947, 28, pp 155-162
Abstract

A method adopted for the cultivation of the F. & M. disease virus on deep explants of bovine tongue mucosa is described, and an account given of the histological changes which are observed.
These changes, occurring in the absence of a vascular system, closely resemble those seen in the...

Author(s)
FRENKEL, H. S.
Citation
American Journal of Veterinary Research, 1949, 10, pp 142-145
Abstract

Virus from a field case of F. & M. disease was found to be type O (Vallée), and was pathogenic for g. pigs. It was grown in cattle embryo tissue cultures, and a tissue culture diluted 1: 10, 000 was pathogenic for g. pigs, causing generalization 48 hours after injection into the tongue.
In...

Author(s)
FRENKEL, H. S.; VAN WAVEREN, G. M.
Citation
Tijdschrift voor Diergeneeskunde, 1938, 65, pp 264-281
Abstract

I. Certain technical problems are discussed and the results of experiments are described. Bacterial contamination of tissue cultures of F. & M. disease virus can be prevented by immersing the infected skin tissue for a few minutes in 70% alcohol held at -15°C. The culture is then removed and...

Author(s)
FRENKEL, H. S.; VAN WAVEREN, G. M.
Citation
Verslagen werkzaam. Staatsveearts. Onderzoek.-Inst., 1938, pp 22-31 pp.
Abstract

I. Since the work described in the previous abstract was done the culture has been further grown over a total of 27 passages in 104 days. Fowl plasma was used in the cultures but, as it is troublesome to obtain, an attempt was made to use agar (0.75 per cent. in Ringer's fluid) as a substitute:...

Author(s)
FRENKEL, H. S.; VAN WAVEREN, G. M.
Publisher
The Hague: Algemeene Landsdrukkerij.,
Citation
Staatsveearts. Onderzoek. -inst., 1935, pp 18 pp.