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A modified direct complement fixation method for the detection of antibodies in heat-inactivated sera is described. The addition of fresh unheated bovine serum to the diluted guineapig complement constitutes the only modification: in its absence no fixation of complement is observed. The normal...

Boulanger, P.; Bannister, G. L.
Journal of Immunology, 1960, 85, pp 368-374

The complement-fixation test used at Onderstepoort was compared with that used at Animal Disease Research Institute at Hull, Quebec (ADRI) on infected calf and sheep serum. In the first method, the tests are incubated at 37°C for 90 min. and the test serum samples are inactivated at 53 °C; whereas...

Gray, D. P.; Willis, N. G.; Girand, A.; Ruckerbauer, G. M.; Boulanger, P.; Bannister, G. L.
Canadian Journal of Comparative Medicine, 1967, 31, pp 182-188