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VetMed Resource

Veterinary information to support practice, based on evidence and continuing education

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Abstract

939 raw milk samples from 54 farms were analysed for the presence of Listeria monocytogenes by cold enrichment vs. flow cytometry, which incorporated a selective enrichment step. Analysed samples consisted of milk from individual farm bulk tanks, string samples that represented milk from 25-40 cows ...

Author(s)
Donnelly, C. W.; Baigent, G. J.; Briggs, E. H.
Citation
Journal - Association of Official Analytical Chemists, 1988, 71, 3, pp 655-658
Abstract

After an outbreak of listeriosis in Massachusetts (USA) in 1983, the ability of Listeria monocytogenes to survive in raw and pasteurized milk was investigated. An enrichment broth (EB) containing acriflavine, nalidixic acid and cycloheximide was used to eliminate overgrowth of the culture by...

Author(s)
Lovett, J.
Citation
Journal - Association of Official Analytical Chemists, 1988, 71, 3, pp 658-660
Abstract

Selective enrichment broths used by the FDA and the USDA, Food Safety and Inspection Service, were compared for their efficiency in the quantitative recovery of Listeria monocytogenes from a naturally contaminated Brie cheese that was obtained as part of an epidemic investigation. Quantitative...

Author(s)
Swaminathan, B.; Hayes, P. S.; Przybyszewski, V. A.
Citation
Journal - Association of Official Analytical Chemists, 1988, 71, 3, pp 664-668
Abstract

A nucleic acid hybridization assay was developed for Listeria spp. in milk products and environmental samples. The assay is based on detection of unique Listeria 16S rRNA sequences by using a 32P-labelled synthetic DNA probe. Inclusivity and exclusivity of the probe were confirmed with 139 Listeria ...

Author(s)
Klinger, J. D.; Johnson, A.; Croan, D.; Flynn, P.; Whippie, K.; Kimball, M.; Lawrie, J.; Curiale, M.
Citation
Journal - Association of Official Analytical Chemists, 1988, 71, 3, pp 669-673
Abstract

A DNA probe was used to identify haemolytic Listeria monocytogenes in naturally contaminated milk products. Foods were homogenized, diluted, and dilutions were spread-plated on LPM agar and incubated overnight for use in the DNA colony hybridization assay. The probe used was a 32P-labelled...

Author(s)
Datta, A. R.; Wentz, B. A.; Hill, W. E.
Citation
Journal - Association of Official Analytical Chemists, 1988, 71, 3, pp 673-675