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An ELISA for antibodies to Hypoderma bovis and H. lineatum was positive in spring 1985 in 1.5% of 6072 cattle on 34 farms in Dorset, and none of 300 cattle in North Yorkshire (an area known to be free from the parasite).
The application of immunological methods to the early diagnosis of bovine hypodermosis has been developed since 1970 when a specific antigen was characterized from the 1st instar larvae of Hypoderma bovis and H. lineatum. Enzyme-linked immunosorbent assay (ELISA) has progressively supplanted many...
A method is described by which the enzyme-linked immunosorbent assay (ELISA) technique can be adapted for the detection of larvae of Hypoderma bovis (L.) in bovines and possibly in man.
This paper is of interest inasmuch as the methods that have been employed for the eradication of the gross animal parasites in the United States are briefly reviewed and the possibilities of further success based on our present knowledge of the life histories of the parasites are discussed. The...