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Abstract

An antigen was prepared from metabolic products which were produced by maintaining 1st instar larvae of Hypoderma lineatum on RPMI tissue culture medium for 48 h. The antigen compared favourably with a soluble extract of larvae when used in an enzyme-linked immunosorbent assay (ELISA).

Author(s)
Wassall, D. A.; Martinez Moreno, J.; Hernandez Rodrigues, S.
Publisher
Office for Official Publications of the European Community, Luxembourg, Luxembourg
Citation
Improvements in control methods for warble-fly in cattle and goats: workshops held in Cordoba 8 to 10 May 1991., 1992, pp 133-140
Abstract

The application of immunological methods to the early diagnosis of bovine hypodermosis has been developed since 1970 when a specific antigen was characterized from the 1st instar larvae of Hypoderma bovis and H. lineatum. Enzyme-linked immunosorbent assay (ELISA) has progressively supplanted many...

Author(s)
Boulard, C.; Villejoubert, C.; Chabaudie, N.
Publisher
Office for Official Publications of the European Community, Luxembourg, Luxembourg
Citation
Improvements in control methods for warble-fly in cattle and goats: workshops held in Cordoba 8 to 10 May 1991., 1992, pp 101-113