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VetMed Resource

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Abstract

From 1999 to 2000, a total of 247 blood samples were collected from 214 cattle and 33 water buffaloes in 16 distinct geographical locations of Thailand and analysed by allele-specific PCR amplification of major piroplasm surface protein (MPSP) genes of benign Theileria parasites. Four allelic MPSP...

Author(s)
Sarataphan, N.; Kakuda, T.; Chansiri, K.; Onuma, M.
Publisher
Japanese Society of Veterinary Science, Tokyo, Japan
Citation
Journal of Veterinary Medical Science, 2003, 65, 1, pp 133-135
Abstract

Equal numbers of grazing Holstein (n=65), Korean native (n=65) and crossbred cattle (n=65) on Cheju island, Korea Republic, were examined for Theileria sergenti infection and parasitaemia levels by microscopic examination of Giemsa-stained blood smears and the polymerase chain reaction (PCR). The...

Author(s)
Kim GonHyung; Lee KyoungKap; Onuma, M.
Citation
Journal of Protozoology Research, 1999, 9, 3, pp 103-112
Abstract

To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitological, serological, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair...

Author(s)
Baek, B. K.; Soo, K. B.; Kim, J. H.; Hur Jin; Lee, B. O.; Jung, J. M.; Onuma, M.; Oluoch, A. O.; Kim, C. H.; Kakoma, I.
Publisher
Canadian Veterinary Medical Association, Ottawa, Canada
Citation
Canadian Journal of Veterinary Research, 2003, 67, 4, pp 278-282
Abstract

A survey of Theileria parasite infection in cattle in Cambodia and Vietnam was carried out using allele-specific polymerase chain reaction. A total of 137 blood samples from draught animals in Cambodia and 40 blood samples from dairy cattle in Vietnam were analysed. In Cambodia, 69 out of 137...

Author(s)
Inoue, M.; Nguyen, D. van; Meas, S.; Ohashi, K.; Sen, S.; Sugimoto, C.; Onuma, M.
Publisher
Japanese Society of Veterinary Science, Tokyo, Japan
Citation
Journal of Veterinary Medical Science, 2001, 63, 10, pp 1155-1157
Abstract

In order to investigate the transmission dynamics of Theileria parva (T. parva) by the brown ear tick, Rhipicephalus appendiculatus (R. appendiculatus), under experimental conditions, detection of T. parva in ticks and cattle was performed by a quantitative real-time PCR assay. A calf inoculated...

Author(s)
Konnai, S.; Imamura, S.; Nakajima, C.; Witola, W. H.; Yamada, S.; Simuunza, M.; Nambota, A.; Yasuda, J.; Ohashi, K.; Onuma, M.
Publisher
Elsevier, Amsterdam, Netherlands
Citation
Acta Tropica, 2006, 99, 1, pp 34-41
Abstract

A total of 491 blood samples, 105 from the south and 386 from the north of Taiwan, were collected from cattle in 16 different farms. From northern blood samples, Theileria piroplasms were seen in only 4, microscopically. p32/34 genes (encoding immunodominant piroplasm surface proteins) were...

Author(s)
Wang ChunTshen; Kubota, S.; Kakuda, T.; Kuo ChinChi; Hsu-TienLai; Onuma, M.
Citation
Journal of Veterinary Medical Science, 1998, 60, 2, pp 253-255
Abstract

To ascertain the infection rate for tick-borne pathogens in Zambia, an epidemiological survey of Theileria parva, Babesia bigemina and Anaplasma marginale in traditionally managed Sanga cattle was conducted using PCR. Of the 71 native Zambian cattle, 28 (39.4%) were positive for T. parva, 16...

Author(s)
Yamada, S.; Konnai, S.; Imamura, S.; Simuunza, M.; Chembensofu, M.; Chota, A.; Nambota, A.; Onuma, M.; Ohashi, K.
Publisher
Elsevier, Amsterdam, Netherlands
Citation
Veterinary Journal, 2009, 182, 2, pp 352-355
Abstract

Theilerial DNA samples were collected from various East Asian countries, including Japan, Korea, Taiwan and China. DNA sequences encoding a major piroplasm surface protein were amplified by the polymerase chain reaction, followed by cloning into a plasmid vector. More than 20 DNA clones derived...

Author(s)
Kim SamJu; Tsuji, M.; Kubota, S.; Wei, Q.; Lee JooMuk; Ishihara, C.; Onuma, M.
Citation
International Journal for Parasitology, 1998, 28, 8, pp 1219-1227
Abstract

Benign Theileria species distributed in China and Korea were characterized by allele-specific polymerase chain reaction (PCR), based on the sequences of major immunodominant piroplasm surface protein genes. In China, all the isolates contained Chitose (C) type parasites. One out of 5 isolates...

Author(s)
Kakuda, T.; Kubota, S.; Sugimoto, C.; Baek, ByeongKirl; Ying, H.; Onuma, M.
Citation
Journal of Veterinary Medical Science, 1998, 60, 2, pp 237-239
Abstract

Theileria parva, the agent of East Coast fever (ECF), is transmitted to the host during the blood meal feeding of Rhipicephalus appendiculatus ticks. In order to investigate the relationship between the attachment duration of R. appendiculatus and the transmission of T. parva, infected adult ticks...

Author(s)
Konnai, S.; Yamada, S.; Imamura, S.; Simuunza, M.; Chembensof, M.; Chota, A.; Nambota, A.; Ohashi, K.; Onuma, M.
Publisher
Mary Ann Liebert, Inc., New Rochelle, USA
Citation
Vector Borne and Zoonotic Diseases, 2007, 7, 2, pp 241-248

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