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Abstract

An overview of the chemotherapy research programme against trypanosomiasis undertaken at the International Laboratory for Research on Animal Diseases in Kenya, is given. Work carried out is grouped under the following 8 broad topics: development of in vitro culture techniques for identifying and...

Author(s)
Peregrine, A. S.; Sutherland, I. A.; Mutharia, L.; Jamnadass, R.; Gray, M. A.; Codjia, V.; Aliu, Y. O.; Mamman, M.; Silayo, R.; D'Ieteren, G. D. M.; Moloo, S. K.; Murphy, N. B.
Publisher
International Laboratory for Research on Animal Diseases, Nairobi, Kenya
Citation
Chemotherapy for trypanosomiasis. Proceedings of a workshop held at ILRAD, Nairobi, Kenya, 21-24 August, 1989., 1990, pp 3-8
Abstract

The sensitivity and specificity of a Trypanosoma vivax DNA-based assay (using a cDNA sequence cloned in pcDSIL-800 which encodes an antigen recognized by MAb Tv27) is reported. Oligonucleotide primers were used in amplifying DNA sequences from the blood of cattle infected with T. vivax isolates...

Author(s)
Masake, R. A.; Majiwa, P. A. O.; Moloo, S. K.; Makau, J. M.; Njuguna, J. T.; Maina, M.; Kabata, J.; ole-MoiYoi, O. K.; Nantulya, V. M.
Citation
Experimental Parasitology, 1997, 85, 2, pp 193-205
Abstract

The sensitivity and specificity of the FAO/IAEA antigen-ELISA kits for diagnosis of bovine trypanosomiasis were investigated using sera from Boran cattle (Bos indicus) in Kenya experimentally infected by tsetse challenge with cloned populations of Trypanosoma congolense (3 populations from...

Author(s)
Eisler, M. C.; Lessard, P.; Masake, R. A.; Moloo, S. K.; Peregrine, A. S.
Citation
Veterinary Parasitology, 1998, 79, 3, pp 187-201
Abstract

Local skin reactions (termed chancres) were elicited in susceptible cattle and goats after challenge with Glossina morsitans morsitans infected with Trypanosoma congolense, T. brucei or T. vivax. The development of the chancre and the detection of parasites within the lesion preceded the appearance ...

Author(s)
Emery, D. L.; Akol, G. W. O.; Murray, M.; Morrison, W. I.; Moloo, S. K.
Publisher
Elsevier/North Holland Biomedical Press., Amsterdam, Netherlands
Citation
The host-invader interplay. Proceedings of the Third International Symposium on the Biochemistry of Parasites and Host-Parasite Relationships, Beerse, Belgium, 30 June-3 July, 1980, 1980, pp 345-356
Abstract

Six Boran cattle were infected with the drug-resistant T. congolense IL 3338. At the first peak of parasitaemia different groups of 200 teneral G. m. centralis were fed once on each animal, just before treatment with diminazene aceturate at a dose of 3.5 mg/kg body weight. Thereafter, all animals...

Author(s)
Diack, A.; Moloo, S. K.; Peregrine, A. S.
Citation
Revue d'Élevage et de Médecine Vétérinaire des Pays Tropicaux, 1998, 51, 3, pp 211-217
Abstract

The 2 populations of G. pallidipes used originated from Nguruman, Rift Valley Province, and Shimba Hills, Coast Province, Kenya, and were from the production colonies bred at ILRAD, Kenya. The T. vivax stocks used were originally isolated from zebu cattle at Galana, Kenya, in 1978, and Zaria,...

Author(s)
Moloo, S. K.; Okumu, I. O.
Citation
Medical and Veterinary Entomology, 1995, 9, 2, pp 202-204
Abstract

Relatively simple protocols employing non-radioactive DNA probes were used for the detection of African trypanosomes (Trypanosoma congolense, T. simiae, T. brucei) in the blood of mammalian hosts and the saliva of live tsetse flies (Glossina spp.). In combination with the polymerase chain reaction, ...

Author(s)
Majiwa, P. A. O.; Thatthi, R.; Moloo, S. K.; Nyeko, J. H. P.; Otieno, L. H.; Maloo, S.
Citation
Parasitology, 1994, 108, 3, pp 313-322
Abstract

Two Trypanosoma vivax stocks (CP 2171 and CP 2331, both isolated from cattle in Kenya) were initiated in culture with tsetse or culture-derived metacyclics. They were propagated axenically as bloodstream trypomastigotes at 35°C in 4% CO2 in air. Populations of trypanosomes were incubated with...

Author(s)
Zweygarth, E.; Kaminsky, R.; Moloo, S. K.
Citation
Onderstepoort Journal of Veterinary Research, 1994, 61, 2, pp 189-191
Abstract

African buffalo (Synceros caffer) and trypanotolerant N'Dama cattle were compared for their resistance to Trypanosoma congolense (3 buffaloes and 3 cattle) and T. vivax (2 buffaloes which had been used in the first experiment 12 months earlier and 2 naive ones, and 4 naive cattle). All animals were ...

Author(s)
Olubayo, R. O.; Moloo, S. K.; Naessens, J.
Citation
Bulletin of Animal Health and Production in Africa, 1996, 44, 1, pp 23-32
Abstract

Author(s)
Williams, D. J. L.; Naessens, J.; Moloo, S. K.; Scott, J. R.
Citation
Publication - International Scientific Council for Trypanosomiasis Research and Control, 1991, No.115, pp 152-161

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