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Theileria annulata schizonts cultured for 13, 54 or 85 days produced mild reactions when inoculated into calves and no mortality, whereas freshly harvested schizonts killed one of 5 (1 × 106 schizonts), 2 of 4 (1 × 105) and 2 of 4 (1 × 104) calves.

Gill, B. S.; Bhattacharyulu, Y.; Kaur, D.; Singh, A.; Gill, H. S.; Samantaray, S. N.
Department of Veterinary Medicine, Haryana Agricultural University, Hissar 125004, Haryana, India
Haemoprotozoan diseases of domestic animals. (Proc. seminar (CwVA Asian/Australian regions), Haryana Agric. Univ., Hissar, India, 27 Oct. to 1 Nov. 1980.), 1983, pp 90