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The test was carried out in 1% Difco agar or 1% ordinary agar containing 0.003% methyl orange and 0.25% phenol; 18 ml agar was poured on a horizontally fixed glass plate 9 × 12 cm divided into 3 equal fields. The peripheral wells, 3-5 mm diam., were 5-10 mm from the central well containing antigen. ...

Onufriev, V. P.; Kravets, I. K.; Shvetsov, Yu. F.; Prostyakov, A. P.; Luboshnikov, A. G.; Dudnikov, A. I.
Voprosy vet. Virus, 1966, 2, pp 129-134

Whey was prepared from individual and bulked milk from 100-200 cows that had recovered from F & M disease (virus type A, variant Ai) 24-105 days previously. Casein was precipitated with citric acid and filtered off. Sodium bicarbonate was added to the whey to give a pH between 6 and 7. It was then...

Onufriev, V. P.; Dudnikov, A. I.; Zakharov, V. M.; Pronin, I. A.; Shvetsov, Yu. F.; Krav-Chenko, V. M.
Veterinariya, 1966, 43, 9, pp 24-28 pp.