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Abstract

Diagnosis of bovine intramammary infection (IMI) has traditionally been based on bacterial culture, but currently IMI can also be detected with DNA based methods, such as multiplex real-time PCR. The aim of this study was to describe the elimination of bacteria in experimentally induced IMI on the...

Author(s)
Hiitiö, H.; Pyörälä, S.; Taponen, S.; Rajala-Schultz, P.; Simojoki, H.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2018, 101, 6, pp 5267-5276
Abstract

Two methods of collecting milk samples from mastitic bovine mammary quarters were compared. Samples were taken in a consistent order in which standard aseptic technique sampling was done first, followed by insertion of a sterile cannula through the teat canal and collection of a second sample....

Author(s)
Friman, M.; Hiitiö, H.; Niemi, M.; Holopainen, J.; Pyörälä, S.; Simojoki, H.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Veterinary Journal, 2017, 226, pp 57-61
AbstractFull Text

Background: The Finnish dairy herd recording system maintains production and health records of cows and herds. Veterinarians and farmers register veterinary treatments in the system. Milk samples for microbiological analysis are routinely taken from mastitic cows. The laboratory of the largest...

Author(s)
Vakkamäki, J.; Taponen, S.; Heikkilä, A. M.; Pyörälä, S.
Publisher
BioMed Central Ltd, London, UK
Citation
Acta Veterinaria Scandinavica, 2017, 59, 33, pp (25 May 2017)
Abstract

Reliable identification of the aetiological agent is crucial in mastitis diagnostics. Real-time PCR is a fast, automated tool for detecting the most common udder pathogens directly from milk. In this study aseptically taken quarter milk samples were analysed with a real-time PCR assay (Thermo...

Author(s)
Hiitiö, H.; Riva, R.; Autio, T.; Pohjanvirta, T.; Holopainen, J.; Pyörälä, S.; Pelkonen, S.
Publisher
Cambridge University Press, Cambridge, UK
Citation
Journal of Dairy Research, 2015, 82, 2, pp 200-208
Abstract

The coagulase-negative staphylococcal species causing mastitis in lactating cattle were identified and possible differences in the clinical characteristics or persistence of mastitis caused by different CNS were evaluated. The effect of antimicrobial treatment was also assessed. In addition,...

Author(s)
Taponen, S.; Simojoki, H.; Haveri, M.; Larsen, H. D.; Pyörälä, S.
Publisher
Elsevier, Amsterdam, Netherlands
Citation
Veterinary Microbiology, 2006, 115, 1/3, pp 199-207
AbstractFull Text

Author(s)
Pyorala, S.
Publisher
The North American Veterinary Conference, Gainesville, USA
Citation
The North American Veterinary Conference 2003, Large Animal. Orlando, Florida, USA, 18-22 January, 2003, 2003, pp 78-79
Abstract

In more than 30% of milk samples from clinical and subclinical bovine mastitis, bacteria fail to grow even after 48 h of conventional culture. The "no-growth" samples are problematic for mastitis laboratories, veterinarians, and dairy producers. This study provides the first investigation of the...

Author(s)
Taponen, S.; Salmikivi, L.; Simojoki, H.; Koskinen, M. T.; Pyörälä, S.
Publisher
American Dairy Science Association, Savoy, USA
Citation
Journal of Dairy Science, 2009, 92, 6, pp 2610-2617

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