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Abstract

Diagnosis of bovine intramammary infection (IMI) has traditionally been based on bacterial culture, but currently IMI can also be detected with DNA based methods, such as multiplex real-time PCR. The aim of this study was to describe the elimination of bacteria in experimentally induced IMI on the...

Author(s)
Hiitiö, H.; Pyörälä, S.; Taponen, S.; Rajala-Schultz, P.; Simojoki, H.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2018, 101, 6, pp 5267-5276
Abstract

The aim of the study was to evaluate the effect of sampling technique on the microbiological results of bovine milk samples using multiplex real-time PCR. Comparison was made between a technique where the milk sample was taken directly from the udder cistern of the udder quarter using a needle and...

Author(s)
Hiitiö, H.; Simojoki, H.; Kalmus, P.; Holopainen, J.; Pyörälä, S.; Taponen, S.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2016, 99, 8, pp 6532-6541
Abstract

The aim of this study was to analyze bacterial profiles of bovine mastitic milk samples and samples from healthy quarters using Next Generation Sequencing of amplicons from 16S rRNA genes and to compare results with microbiological results by PCR assays of the same samples. A total of 49 samples...

Author(s)
Taponen, S.; McGuinness, D.; Hiitiö, H.; Simojoki, H.; Zadoks, R.; Pyörälä, S.
Publisher
BioMed Central Ltd, London, UK
Citation
Veterinary Research, 2019, 50, 44, pp (06 June 2019)
AbstractFull Text

Persistence of coagulase-negative staphylococci in intramammary infection during lactation was studied. Milk samples from 328 udder quarters of 82 dairy cows were collected every 4 weeks until the end of lactation. CNS isolates were analyzed with API Staph ID 32 test and genotyped using amplified...

Author(s)
Taponen, S.; Koort, J.; Björkroth, J.; Saloniemi, H.; Pyörälä, S.
Publisher
Estonian University of Life Sciences, Jõgeva Plant Breeding Institute, Estonian Research Institute of Agriculture, Tartu, Estonia
Citation
Animal health, animal welfare and biosecurity. Proceedings of 13th International Congress in Animal Hygiene, Tartu, Estonia, 17-21 June, 2007. volume 1 , 2007, pp 476-479
Abstract

Reduction in long-term milk yields represents a notable share of the economic losses caused by bovine mastitis. Efficient, economic, and safe measures to prevent these losses require knowledge of the causal agent of the disease. The aim of this study was to investigate pathogen-specific impacts of...

Author(s)
Heikkilä, A. M.; Liski, E.; Pyörälä, S.; Taponen, S.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2018, 101, 10, pp 9493-9504
AbstractFull Text

Background: Coagulase-negative staphylococci (CoNS) are the most common bovine mastitis causing bacteria in many countries. It is known that resistance for antimicrobials is in general more common in CoNS than in Staphylococcus aureus but little is known about the antimicrobial resistance of...

Author(s)
Taponen, S.; Nykäsenoja, S.; Pohjanvirta, T.; Pitkälä, A.; Pyörälä, S.
Publisher
BioMed Central Ltd, London, UK
Citation
Acta Veterinaria Scandinavica, 2016, 58, 12, pp (6 February 2016)
Abstract

The aim of this study was to determine risk factors for bovine intramammary infection (IMI) associated with the most common bacterial species in Finland. Large databases of the Finnish milk-recording system and results of microbiological analyses of mastitic milk samples from Valio Ltd. (Helsinki,...

Author(s)
Taponen, S.; Liski, E.; Heikkilä, A. M.; Pyörälä, S.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2017, 100, 1, pp 493-503
AbstractFull Text

Background: The Finnish dairy herd recording system maintains production and health records of cows and herds. Veterinarians and farmers register veterinary treatments in the system. Milk samples for microbiological analysis are routinely taken from mastitic cows. The laboratory of the largest...

Author(s)
Vakkamäki, J.; Taponen, S.; Heikkilä, A. M.; Pyörälä, S.
Publisher
BioMed Central Ltd, London, UK
Citation
Acta Veterinaria Scandinavica, 2017, 59, 33, pp (25 May 2017)
Abstract

An experimental infection model was developed to study host response to intramammary infection in cows caused by Staphylococcus chromogenes. CNS intramammary infections have become very common in modern dairy herds, and they can remain persistent in the mammary gland. More information would be...

Author(s)
Simojoki, H.; Orro, T.; Taponen, S.; Pyörälä, S.
Publisher
Elsevier, Amsterdam, Netherlands
Citation
Veterinary Microbiology, 2009, 134, 1/2, pp 95-99
Abstract

Fast and reliable identification of the microorganisms causing mastitis is important for management of the disease and for targeting antimicrobial treatment. Methods based on PCR are being used increasingly in mastitis diagnostics. Comprehensive field comparisons of PCR and traditional milk...

Author(s)
Koskinen, M. T.; Wellenberg, G. J.; Sampimon, O. C.; Holopainen, J.; Rothkamp, A.; Salmikivi, L.; Haeringen, W. A. van; Lam, T. J. G. M.; Pyörälä, S.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2010, 93, 12, pp 5707-5715

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