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Abstract

The fluorescence polarization assay (FPA) was evaluated for the serological diagnosis of bovine brucellosis. 118 sera from cattle which were culture positive for Brucella abortus, 1751 sera from cattle from premises containing cattle infected with B. abortus, 1222 sera from cattle vaccinated with ...

Author(s)
Nielsen, K.; Gall, D.; Lin, M.; Massangill, C.; Samartino, L.; Perez, B.; Coats, M.; Hennager, S.; Dajer, A.; Nicoletti, P.; Thomas, F.
Citation
Veterinary Immunology and Immunopathology, 1998, 66, 3/4, pp 321-329
Abstract

A fluorescence polarization assay (FPA) was used to test (198, Canada; 32, Argentina; 108, Chile and 85, Ireland) whole blood samples prepared by mixing blood cells from cattle without exposure to Brucella abortus with 691 sera from animals with confirmed (bacteriologically) infection. A cutoff...

Author(s)
Nielsen, K.; Gall, D.; Smith, P.; Kelly, W.; Yeo, J.; Kenny, K.; Heneghan, T.; McNamara, S.; Maher, P.; O'Connor, J.; Walsh, B.; Carroll, J.; Rojas, X.; Rojas, F.; Perez, B.; Wulff, O.; Buffoni, L.; Salustio, E.; Gregoret, R.; Samartino, L.; Dajer, A.; Luna-Martinez, E.
Publisher
Elsevier Science B.V., Amsterdam, Netherlands
Citation
Veterinary Microbiology, 2001, 80, 2, pp 163-170
Abstract

An indirect enzyme immunoassay for detection of antibody to B. abortus in bovine milk was developed and validated using 6238 milk samples from Canadian herds (brucellosis free) and 202 samples from herds infected with B. abortus (from Argentina and Chile). The assay utilized lipopolysaccharide as...

Author(s)
Nielsen, K.; Smith, P.; Gall, D.; Pérez, B.; Cosma, C.; Mueller, P.; Trottier, J.; Cote, G.; Boag, L.; Bosse, J.
Citation
Veterinary Microbiology, 1996, 52, 1/2, pp 165-173