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Abstract

A study was conducted to evaluate the use of the polymerase chain reaction (PCR) in the differential diagnosis of T. vivax isolates from cattle in Brazil. Results showed that both conventional PCR and RFLP (restriction fragment length polymorphism)-PCR could differentiate between T. vivax and T....

Author(s)
Madruga, C. R.; Araújo, F. R. de; Soares, C. O.; Melo, E. S. de P.; Almeida, D. A.; Almeida Júnior, N. F. de; Xavier, M. A. S.; Osório, A. L. A.; Góes-Cavalcante, G.; Ramos, C. A. do N.
Publisher
Embrapa Gado de Corte, Campo Grande, Brazil
Citation
Comunicado Técnico - Embrapa Gado de Corte, 2003, No.84, pp 5 pp.
Abstract

The seroprevalence of Anaplasma marginale among dairy cattle in Goînia, Goias, Brazil, using indirect fluorescent antibody test (IFAT) and ELISA, was determined through an experimental study [date not given]. The map of the area was divided into 47 squares , from which 25 were chosen to be visited...

Author(s)
Santos, H. Q. dos; Madruga, C. R.; Linhares, G. F. C.
Publisher
Faculdade de Veterinaria, Universidade Federal Fluminense, Niterói, Brazil
Citation
Revista Brasileira de Ciência Veterinária, 2001, 8, 1, pp 31-34
Abstract

Seroprevalence of antibodies against Anaplasma marginale were studied using an indirect ELISA. Serum samples from 223 cattle from 8 municipalities in the Médio Paraíba mesoregion in the state of Rio de Janeiro, Brazil, were analysed. 98.21% of the samples were positive: 36.32% had a titre of 1:500, ...

Author(s)
Souza, J. C. P de; Soares, C. O.; Madruga, C. R.; Massard, C. L.
Publisher
Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, Brazil
Citation
Ciência Rural, 2001, 31, 2, pp 309-314
Abstract

The cattle parasitic tristeza (TPB; Texas Fever), caused by Babesia bovis, B. bigemina, and Anaplasma marginale, has been known since the end of the 19th century. Preimmunization (infection/treatment) has been used since the beginning of the 20th century as a prophylactic method. In the 60s and...

Author(s)
Kessler, R. H.; Soares, C. O.; Madruga, C. R.; Araújo, F. R. de
Publisher
EMBRAPA Gado de Corte, Campo Grande, Brazil
Citation
Documentos - Embrapa Gado de Corte, 2002, No.131, pp 27 pp.
Abstract

A serological survey was conducted to determine the prevalence of IgG antibodies against B. bovis and B. bigemina. 2018 sera from four municipalities in Rio de Janeiro were collected and analysed by the indirect fluorescent antibodies technique (IFAT). Overall, 82.11 and 94.03% were positive. 83...

Author(s)
Madruga, C. R.; Braga, M. M.; Oliveira, D. B. de; Massard, C. L.; Soares, C. O.
Publisher
Faculdade de Veterinaria, Universidade Federal Fluminense, Niteroi, Brazil
Citation
Revista Brasileira de Ciência Veterinária, 2000, 7, 2, pp 113-116
Abstract

The goal of this research was to determine the frequency of anti-Babesia bovis and anti-B. bigemina IgG in cattle from Paudalho County, Pernambuco State, Northeast of Brazil. A total of 168 samples of bovine serum were analysed by immunofluorescence indirect antibody test for the detection of IgG...

Author(s)
Berto, R. S.; Faustino, M. A. G.; Melo, L. E. H.; Alves, L. C.; Madruga, C. R.; Almeida, M. A. O.; Ramos, C. A. N.; Tenório, T. G. S.; Silva, F. F.
Publisher
Universidade Federal Rural de Pernambuco, Recife, Brazil
Citation
Medicina Veterinária (Brasil), 2008, 2, 3, pp 9-12
Abstract

Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed for detecting antibodies against this rickettsia in cattle. The high sensitivities (99% for both tests) and specificities (100% for both tests)...

Author(s)
Araújo, F. R. de; Melo, V. S. P. de; Ramos, C. A. do N.; Madruga, C. R.; Soares, C. O.; Kessler, R. H.; Almeida, N. F. de; Araújo, G. S. de; Alves, L. C.; Torres Júnior, R. A. de A.; Fragoso, S. P.; Arauco, P. R. C.; Bacenelli, G.; Oliveira, M. B. de
Publisher
Embrapa Gado de Corte, Campo Grande, Brazil
Citation
Boletim de Pesquisa e Desenvolvimento - Embrapa Gado de Corte, 2004, No.17, pp 24 pp.
Abstract

In this study, the transcription profile of membrane protein gene of three Brazilian isolates of Anaplasma marginale, obtained in Rio Grande do Norte, Pernambuco-Zona da Mata and Pernambuco-Sertão, was shown. RNA was purified from cattle blood experimentally infected with the three isolates of A....

Author(s)
Ramos, C. A. N.; Araújo, F. R.; Osório, A. L. A. R.; Madruga, C. R.; Rosinha, G. M. S.; Soares, C. O.; Elisei, C.
Publisher
Colégio Brasileiro de Parasitologia Veterinária, São Paulo, Brazil
Citation
Revista Brasileira de Parasitologia Veterinária, 2007, 16, 3, pp 152-155
Abstract

The objective of this study was the production and solubilization of recombinant truncated MSP5 of Anaplasma marginale and the evaluation of its performance in an enzyme-linked immunosorbent assay (ELISA), to detect antibodies against the rickettsia in cattle. The fragment of msp5 gene, except the...

Author(s)
Melo, E. S. P.; Araújo, F. R.; Ramos, C. A. N.; Soares, C. O.; Rosinha, G. M. S.; Elisei, C.; Madruga, C. R.
Publisher
Colégio Brasileiro de Patologia Animal, Rio de Janeiro, Brazil
Citation
Pesquisa Veterinária Brasileira, 2007, 27, 7, pp 301-306
Abstract

A comparative study on the clinical and haematological alterations caused by isolates of Babesia bigemina from Southeastern, Northeastern, and Northern Brazil in experimentally infected Nelore calves, was conducted. 18 calves between 7 and 9 months of age, without antibodies against Babesia sp. and ...

Author(s)
Mendonça, C. L. de; Vieira, D.; Kohayagawa, A.; Schenk, M. A. M.; Madruga, C. R.; Afonso, J. A. B.
Publisher
Colégio Brasileiro de Patologia Animal, Rio de Janeiro, Brazil
Citation
Pesquisa Veterinária Brasileira, 2003, 23, 2, pp 52-60

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