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Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative bacterium of Johne's disease (JD) in ruminants. The control of JD in the dairy industry is challenging, but can be improved with a better understanding of the diversity and distribution of MAP subtypes. Previously established...

Author(s)
Ahlstrom, C.; Barkema, H. W.; Stevenson, K.; Zadoks, R. N.; Biek, R.; Kao, R.; Trewby, H.; Haupstein, D.; Kelton, D. F.; Fecteau, G.; Labrecque, O.; Keefe, G. P.; McKenna, S. L. B.; Tahlan, K.; Buck, J. de
Publisher
Public Library of Sciences (PLoS), San Francisco, USA
Citation
PLoS ONE, 2016, 11, 2, pp e0149017
Abstract

Simple, rapid and cost-effective methods are sought for measuring immunoglobulin G (IgG) concentrations in bovine serum, which can be applied for diagnosis of failure of transfer of passive immunity (FTPI). The aim of the present study was to investigate the potential use of Fourier-transform...

Author(s)
Elsohaby, I.; Riley, C. B.; Hou SiYuan; McClure, J. T.; Shaw, R. A.; Keefe, G. P.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Veterinary Journal, 2014, 202, 3, pp 510-515
Abstract

Background: Following the recent development of a new approach to quantitative analysis of IgG concentrations in bovine serum using transmission infrared spectroscopy, the potential to measure IgG levels using technology and a device better designed for field use was investigated. A method using...

Author(s)
Elsohaby, I.; Hou SiYuan; McClure, J. T.; Riley, C. B.; Shaw, R. A.; Keefe, G. P.
Publisher
BioMed Central Ltd, London, UK
Citation
BMC Veterinary Research, 2015, 11, 218, pp (20 August 2015)
Abstract

The objective of this study was to explore the potential of transmission infrared (TIR) spectroscopy in combination with partial least squares regression (PLSR) for quantification of dairy and beef cow colostral immunoglobulin G (IgG) concentration and assessment of colostrum quality. A total of...

Author(s)
Elsohaby, I.; Windeyer, M. C.; Haines, D. M.; Homerosky, E. R.; Pearson, J. M.; McClure, J. T.; Keefe, G. P.
Publisher
American Society of Animal Science, Savoy, USA
Citation
Journal of Animal Science, 2018, 96, 2, pp 771-782
Abstract

In herds with typical moderate to low within-herd prevalence, testing for Mycobacterium avium ssp. paratuberculosis (MAP), the infectious agent of Johne's disease, will be more cost-effective if individual fecal samples are cultured in composite pools. However, sensitivity to classify a pool...

Author(s)
McKenna, S. L. B.; Ritter, C.; Dohoo, I.; Keefe, G. P.; Barkema, H. W.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2018, 101, 8, pp 7463-7470
Abstract

The objectives of this study were (1) to determine the differences in IgG and total protein (TP) content of serum and plasma samples collected from the same calves; (2) to evaluate the correlation between calf serum and plasma IgG levels, Brix scores, and TP concentrations; (3) to determine whether ...

Author(s)
Elsohaby, I.; McClure, J. T.; Waite, L. A.; Cameron, M.; Heider, L. C.; Keefe, G. P.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2019, 102, 1, pp 567-577
Abstract

Skin lesions are commonly seen in dairy herds and have been associated with animal-, environmental-, and management factors. These lesions are not only a welfare concern, but they also affect profitability. Three areas on the cattle were examined for skin lesions: the hock, knee, and neck. Previous ...

Author(s)
Jewell, M. T.; Cameron, M.; Spears, J.; McKenna, S. L.; Cockram, M. S.; Sanchez, J.; Keefe, G. P.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2019, 102, 4, pp 3376-3391
Abstract

Accurate diagnosis of failure of transfer of passive immunity (FTPI) in newborn calves is an essential component of dairy farm management plan. Several methods (direct and indirect) are available for diagnosis of FTPI in dairy calves. However, the indirect methods offer an advantage over the direct ...

Author(s)
Elsohaby, I.; Mweu, M. M.; Mahmmod, Y. S.; McClure, J. T.; Keefe, G. P.
Publisher
Elsevier B.V., Amsterdam, Netherlands
Citation
Preventive Veterinary Medicine, 2019, 164, pp 72-77
Abstract

In this study, we evaluated and compared the performance of transmission and attenuated total reflectance (ATR) infrared (IR) spectroscopic methods (in combination with quantification algorithms previously developed using partial least squares regression) for the rapid measurement of bovine serum...

Author(s)
Elsohaby, I.; McClure, J. T.; Riley, C. B.; Shaw, R. A.; Keefe, G. P.
Publisher
American Association of Veterinary Laboratory Diagnosticians, Davis, USA
Citation
Journal of Veterinary Diagnostic Investigation, 2016, 28, 1, pp 30-37
Abstract

Milk ELISA are commonly used for detection of Mycobacterium avium ssp. paratuberculosis (MAP) antibodies in dairy cows, due to low cost and quick processing for large numbers of samples. However, low sensitivity and variations from host and environmental factors can impede detection of MAP...

Author(s)
Laurin, E. L.; Sanchez, J.; Chaffer, M.; McKenna, S. L. B.; Keefe, G. P.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2017, 100, 1, pp 598-607

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