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With the use of circulating water systems to rear larvae, Simulium erythrocephalum (Deg.) and S. lineatum (Mg.) were maintained continuously in the laboratory in the United Kingdom for 7 and 3 generations, respectively. Up to 100% insemination was achieved. Using chick skin membranes and fresh or...
In the laboratory in England, the authors successfully obtained 3rd-stage infective larvae of Onchocerca volvulus in a surrogate intermediate host, Simulium ornatum Mg. (a natural vector of O. lienalis of cattle in England). Cryopreserved microfilariae of O. volvulus from skin-snips of patients at...
Quantitative studies were carried out on the cryopreservation of microfilariae of O. gutturosa using infected skin snips obtained from a heavily infected cow umbilicus. Eight to 10 skin snips were used for each experimental treatment. The snips were weighed and individually incubated on ice with...