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Abstract

The serum IgG response of cattle with cysticercosis caused by Taenia saginata was studied in an ELISA in which a T. saginata metacestode surface extract was used as antigen. In experimentally infected calves, a sharp rise in specific antibody levels was found 3-4 weeks after the infection followed...

Author(s)
Kyvsgaard, N. C.; Ilsøe, B.; Henriksen, S. A.; Feld, N. C.; Nansen, P.
Citation
Acta Veterinaria Scandinavica, 1991, 32, 2, pp 233-241
Abstract

An ammonium sulfate fraction of Taenia hydatigena cyst fluid (ThFAS) was further fractionated by hydrophobic interaction chromatography, using alkylagarose and ω-amino alkylagarose columns, in an effort to isolate and purify a specific diagnostic antigen in the ThFAS preparation. The <12 kDa antigen had an affinity for immobilized alkanes with chain length of six carbons or greater. The antigen was recovered in an ethylene glycol eluate from a hexylagarose column then analysed by Western blot; it reacted with bovine and human cysticercosis infection sera and with specific monoclonal antibodies but not with control sera or fascioliasis infection sera. When the eluate was used as coating antigen in a plate ELISA assay no false positive reactions were seen in sera from cattle...

Author(s)
Hayunga, E. G.; Sumner, M. P.
Citation
Veterinary Immunology and Immunopathology, 1991, 28, 1, pp 57-65
Abstract

In an outbreak on one farm 67 of 265 cattle slaughtered during two years had cysticercosis. Counterimmunoelectrophoresis of serum samples from slaughter cattle showed 44.8% sensitivity and 86.4% specificity for homologous Taenia saginata strobilus antigen, compared with 28.3% and 89.9% for...

Author(s)
Lukeš, Š.; Šimečková, A.; Štěrba, J.; Prokopič, J.; Schandl, V.
Publisher
Czech Academy of Agricultural Sciences,
Citation
Veterinární Medicína, 1986, 31, 1, pp 7-13
Abstract

A "crude" antigen prepared from the strobilate stage of Taenia saginata was used in the peroxidase microenzyme-linked immunosorbent assay on serum samples from laboratory and pasture-reared calves, experimentally infected with T. saginata, as well as from cattle naturally infected by grazing on...

Author(s)
Craig, P. S.; Rickard, M. D.
Citation
Zeitschrift fur Parasitenkunde, 1980, 61, 3, pp 287-297
Abstract

A highly immunogenic fraction (F10) isolated from an extract of T. saginata strobilae by immunoaffinity chromatography showed a significant decrease in sensitivity (without a corresponding increase in specificity) in the diagnosis of bovine cysticerciasis by ELISA or counter-immunoelectrophoresis....

Author(s)
Kumar, V.; Geerts, S.; Jochems, M.; Brandt, J.
Publisher
Commission of the European Communities, Luxemburg, France
Citation
Agriculture. Some important parasitic infections in bovines considered from economic and social (zoonosis) points of view. Parasitological symposium, Lyons, 24-26 October 1983., 1984, pp 167-174
Abstract

The diagnosis of bovine cysticerciasis using a standard ELISA (with T. crassiceps antigen) proposed by the EEC Working Group on Cysticercosis is discussed. Antibody detection was rather insensitive, but in both naturally and experimentally infected animals, circulating antigens were detectable soon ...

Author(s)
Van Knapen, F.; Van der Lugt, G.; Franchimont, J. H.
Publisher
Commission of the European Communities, Luxemburg, France
Citation
Agriculture. Some important parasitic infections in bovines considered from economic and social (zoonosis) points of view. Parasitological symposium, Lyons, 24-26 October 1983., 1984, pp 175-181
Abstract

Limitations of serological tests for the diagnosis of bovine cysticerciasis are discussed. It is considered that the practical value of an immunodiagnostic test has been greatly over-exaggerated.

Author(s)
Urquhart, G. M.
Publisher
Commission of the European Communities, Luxemburg, France
Citation
Agriculture. Some important parasitic infections in bovines considered from economic and social (zoonosis) points of view. Parasitological symposium, Lyons, 24-26 October 1983., 1984, pp 183-186
Abstract

Steers sensitized or infected with T. saginata exhibited similar delayed-type dermal hypersensitivity (DTH) responses after intradermal inoculation with T. saginata or T. crassiceps skin-test antigens. Steers sensitized to T. crassiceps cysticerci exhibited similar DTH responses to intradermal...

Author(s)
Hilwig, R. W.; Cramer, J. D.
Citation
Veterinary Parasitology, 1983, 12, 2, pp 155-164
Abstract

Experiments were carried out with eight calves aged 11-14 weeks, experimentally infected with Taenia saginata eggs, to ascertain the optimum conditions for the use of ELISA. An increase in ELISA values was observed after the second to fifth week of infection. At the same time serum antibodies were...

Author(s)
Walther, M.; Sanitz, W.
Citation
Berliner und Münchener Tierärztliche Wochenschrift, 1979, 92, 7, pp 131-135
Abstract

Crude somatic or cyst fluid extracts prepared from Taenia saginata, T. hydatigena or Echinococcus granulosus were partially purified by absorption against homologous and heterologous bovine or ovine antisera on immunoabsorbent affinity columns. Antigens in parasite extracts which were eluted after...

Author(s)
Craig, P. S.; Rickard, M. D.
Citation
International Journal for Parasitology, 1981, 11, 6, pp 441-449

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