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Abstract

An ammonium sulfate fraction of Taenia hydatigena cyst fluid (ThFAS) was further fractionated by hydrophobic interaction chromatography, using alkylagarose and ω-amino alkylagarose columns, in an effort to isolate and purify a specific diagnostic antigen in the ThFAS preparation. The <12 kDa antigen had an affinity for immobilized alkanes with chain length of six carbons or greater. The antigen was recovered in an ethylene glycol eluate from a hexylagarose column then analysed by Western blot; it reacted with bovine and human cysticercosis infection sera and with specific monoclonal antibodies but not with control sera or fascioliasis infection sera. When the eluate was used as coating antigen in a plate ELISA assay no false positive reactions were seen in sera from cattle...

Author(s)
Hayunga, E. G.; Sumner, M. P.
Citation
Veterinary Immunology and Immunopathology, 1991, 28, 1, pp 57-65
Abstract

A "crude" antigen prepared from the strobilate stage of Taenia saginata was used in the peroxidase microenzyme-linked immunosorbent assay on serum samples from laboratory and pasture-reared calves, experimentally infected with T. saginata, as well as from cattle naturally infected by grazing on...

Author(s)
Craig, P. S.; Rickard, M. D.
Citation
Zeitschrift fur Parasitenkunde, 1980, 61, 3, pp 287-297
Abstract

Crude somatic or cyst fluid extracts prepared from Taenia saginata, T. hydatigena or Echinococcus granulosus were partially purified by absorption against homologous and heterologous bovine or ovine antisera on immunoabsorbent affinity columns. Antigens in parasite extracts which were eluted after...

Author(s)
Craig, P. S.; Rickard, M. D.
Citation
International Journal for Parasitology, 1981, 11, 6, pp 441-449
Abstract

The indirect fluorescent antibody (IFA) test using activated oncospheres as antigen was made on the sera of 7 calves experimentally infected with Taenia saginata eggs. On the day of infection the titres ranged from 1:4 to 1:32. Significant titres of 1:64 began to appear by day 7 and reached a peak...

Author(s)
Gathuma, J. M.; Sollod, A. E.; Waiyaki, P. G.
Citation
Kenya Veterinarian, 1978, 2, 1, pp 7-11

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