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Abstract

Continuous or primary epithelial cell lines have been extensively used to study the mammary gland immune response, but they are constituted by a single cell population. Our aim was to test whether an explant of heifer gland, where the tissue structure is maintained, might be a valid model to...

Author(s)
Magro, G.; Brevini, T. A. L.; Maglie, M. de; Minozzi, G.; Scanziani, E.; Piccinini, R.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Research in Veterinary Science, 2017, 114, pp 44-50
Abstract

Twenty-nine strains of mastitis pathogens were used to study the antibacterial activity of the cell-free supernatants (CFS) of 25 strains of Lactococcus lactis ssp. lactis. Out of the tested strains, only the CFS of L. lactis LL11 and SL153 were active, inhibiting and killing most of the pathogens. ...

Author(s)
Malvisi, M.; Stuknytė, M.; Magro, G.; Minozzi, G.; Giardini, A.; Noni, I. de; Piccinini, R.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2016, 99, 3, pp 2288-2296
Abstract

Methicillin-resistant S. aureus (MRSA) lineages have become major responsible of healthcare- and community-associated infections in human population. Bovine MRSA are sporadically detected in the dairy herd, but its presence enhances the risk of zoonosis. Some lineages are able to lose the specific...

Author(s)
Magro, G.; Rebolini, M.; Beretta, D.; Piccinini, R.
Publisher
Elsevier B.V., Amsterdam, Netherlands
Citation
Veterinary Microbiology, 2018, 227, pp 29-33
Abstract

Staphylococcus aureus isolated from mammary gland are characterized by different genetic patterns. Ninety four isolates from 33 dairy herds were analyzed by the means of a microarray to investigate S. aureus virulence patterns and the distribution of genes believed to be involved in immune evasion. ...

Author(s)
Mazzilli, M.; Piccinini, R.; Scali, F.; Zecconi, A.
Publisher
Elsevier Ltd, Oxford, UK
Citation
Research in Veterinary Science, 2015, 103, pp 54-59
Abstract

Staphylococcus aureus is one of the most important causes of mastitis in dairy cattle. Based on previous research, Staph. aureus genotypes with different pathogenic and contagious properties can cause intramammary infection (IMI) and coexist in the same herd. Our study aimed to compare Staph....

Author(s)
Cremonesi, P.; Pozzi, F.; Raschetti, M.; Bignoli, G.; Capra, E.; Graber, H. U.; Vezzoli, F.; Piccinini, R.; Bertasi, B.; Biffani, S.; Castiglioni, B.; Luini, M.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2015, 98, 10, pp 6828-6838
Abstract

Changes in relative cell proportions occurring in diseased mammary glands of dairy cows can be determined using differential cell count (DCC). The present study was carried out in 2 consecutive trials, with 2 goals: (a) to test the consistency of DCC results on subsequent days, and (b) to establish ...

Author(s)
Pilla, R.; Malvisi, M.; Snel, G. G. M.; Schwarz, D.; König, S.; Czerny, C. P.; Piccinini, R.
Publisher
Elsevier Inc., Philadelphia, USA
Citation
Journal of Dairy Science, 2013, 96, 3, pp 1653-1660
Abstract

Staphylococcus aureus isolates from dairy cow mastitis are not always consistent with the characteristic morphology described, and molecular investigation is often needed. The aim of the study was to develop a duplex real-time PCR assay for rapid identification of Staph. aureus isolates, targeting...

Author(s)
Pilla, R.; Snel, G. G. M.; Malvisi, M.; Piccinini, R.
Publisher
Cambridge University Press, Cambridge, UK
Citation
Journal of Dairy Research, 2013, 80, 2, pp 223-226
Abstract

Staphylococcus aureus is one of the most important pathogens associated with bovine mastitis. Recent studies have shown that Staph. aureus strains may differ in virulence, and in their ability to disseminate across commercial dairy herds. The goal of this study was to determine whether Staph....

Author(s)
Piccinini, R.; Tassi, R.; Daprà, V.; Pilla, R.; Fenner, J.; Carter, B.; Anjum, M. F.
Publisher
Cambridge University Press, Cambridge, UK
Citation
Journal of Dairy Research, 2012, 79, 2, pp 249-255
Abstract

Staphylococcus aureus is one of the most common mastitis-causing pathogens worldwide. In the last decade, livestock-associated methicillin-resistant S. aureus (LA-MRSA) infections have been described in several species, included the bovines. Hence, this paper investigates the diffusion of MRSA...

Author(s)
Luini, M.; Cremonesi, P.; Magro, G.; Bianchini, V.; Minozzi, G.; Castiglioni, B.; Piccinini, R.
Publisher
Elsevier B. V., Amsterdam, Netherlands
Citation
Veterinary Microbiology, 2015, 178, 3/4, pp 270-274
Abstract

The biofilm-associated protein (Bap) of Staphylococcus aureus is a high molecular weight cell-wall-anchored protein involved in biofilm formation, first described in bovine mastitis strains from Spain. So far, studies regarding Bap were mainly based on the Spanish strain V329 and its mutants, but...

Author(s)
Snel, G. G. M.; Monecke, S.; Ehricht, R.; Piccinini, R.
Publisher
Cambridge University Press, Cambridge, UK
Citation
Journal of Dairy Research, 2015, 82, 3, pp 312-316

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