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Abstract

Genomic DNA of Staphylococcus spp. was analysed by polymerase chain reaction-based (PCR-based) DNA fingerprinting to distinguish between spp. 123 staphylococci isolated from bovine mammary secretions, and 9 type strains representing 8 spp. were evaluated. Amplified DNA fragments were categorized as ...

Author(s)
Matthews, K. R.; Oliver, S. P.
Citation
Journal of Food Protection, 1994, 57, 6, pp 486-489
Abstract

In tests on 120 strains of staphylococci isolated from milk of Jersey cows in a single herd, an overall susceptibility of 90.5% by the 14 Staphylococcus aureus strains and 92.5% by the other staphylococcal strains was shown in vitro to 12 antibiotics tested. All species were sensitive to...

Author(s)
Matthews, K. R.; Oliver, S. P.; Jayarao, B. M.
Citation
Journal of Dairy Science, 1990, 73, Supplement 1, pp 190
Abstract

A natural exposure trial was conducted for 12 months in a herd of 150 lactating Jersey cows to determine efficacy of a 0.35% chlorhexidine teat dip containing a glycerine emollient for the prevention of bovine intramammary infections. Right teats of cows were dipped in the experimental teat dip...

Author(s)
Oliver, S. P.; King, S. H.; Lewis, M. J.; Torre, P. M.; Matthews, K. R.; Dowlen, H. H.
Citation
Journal of Dairy Science, 1990, 73, 8, pp 2230-2235
Abstract

During a 14-month period, 77 multiparous and 36 primiparous cows were sampled to determine the prevalence of staphylococci during the periparturient period. Distal streak canal swabs were taken at 14 days pre partum, and foremilk was sampled on the first 5 consecutive wk of lactation. ...

Author(s)
Matthews, K. R.; Harmon, R. J.; Langlois, B. E.
Citation
Journal of Dairy Science, 1992, 75, 7, pp 1835-1839
Abstract

Capsule expression was assessed in 6 coagulase-negative staphylococcal strains in serum-soft agar and by India ink and electron microscopy. Classification of strains as encapsulated by serum-soft agar and India ink methods differed. Staphylococcus chromogenes, S. hyicus and S. simulans grew as...

Author(s)
Matthews, K. R.; Oliver, S. P.; Guidry, A. J.
Citation
Journal of Applied Bacteriology, 1991, 71, 1, pp 38-45
Abstract

The susceptibility of 110 strains of Staphylococcus and 140 of Streptococcus isolated from the mammary glands of cows was tested. 99% or more of the staphylococcal strains were sensitive to cephalothin, erythromycin, gentamicin and kanamycin. Of the streptococcal strains 99% or more were sensitive...

Author(s)
Matthews, K. R.; Oliver, S. P.; Jayarao, B. M.
Citation
Agri-Practice, 1992, 13, 3, pp 18-24
Abstract

Plasmid profiles of staphylococci isolated from bovine mammary secretions were heterogeneous as shown by the study of 94 isolates representing 6 species. Plasmids were identified in 19 of 94 staphylococcal isolates. Number of plasmids per isolate varied from 1 to 4. Size of plasmids ranged from 1.2 ...

Author(s)
Matthews, K. R.; Jayarao, B. M.; Oliver, S. P.
Citation
Journal of Dairy Science, 1992, 75, 12, pp 3318-3323
Abstract

Fifty-one staphylococcal isolates from mammary secretions of cows with subclinical mastitis were examined by antibiograms and DNA restriction endonuclease fingerprinting (REF). DNA REF differentiated closely related strains of each species isolated from mammary secretions of different mammary...

Author(s)
Matthews, K. R.; Jayarao, B. M.; Oliver, S. P.
Citation
Epidemiology and Infection, 1992, 109, 1, pp 59-68
Abstract

Overall new infection frequency in uninfected quarters was approx. 2 times greater than in coagulase-negative Staphylococcus infected quarters (P<0.05). New infections by minor pathogens (coagulase-negative staphylococci) were greater (P<0.05) in uninfected quarters than in quarters with...

Author(s)
Matthews, K. R.; Harmon, R. J.
Citation
Journal of Dairy Science, 1989, 72, Suppl. 1, pp 21
Abstract

206 strains of coagulase-negative Staphylococcus species were assessed for expression of glycocalyx on serum soft agar, india ink and adherence techniques. The organisms were maintained on trypticase soya agar plates at 4°C for 30 days (120 strains) or stored at -80°C in skim milk for 90 days (60...

Author(s)
Matthews, K. R.; Oliver, S. P.; King, S. H.
Citation
Journal of Applied Bacteriology, 1991, 70, 3, pp 227-232

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