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Abstract

Accurate identification of mastitis pathogens is often compromised when using conventional culture-based methods. Here, we report a novel, rapid assay tested for speciation of bacterial mastitis pathogens using high-resolution melt analysis (HRMA) of 16S rDNA sequences. Real-time PCR amplification...

Author(s)
Praseeda Ajitkumar; Barkema, H. W.; Buck, J. de
Publisher
Elsevier Ltd, Oxford, UK
Citation
Veterinary Microbiology, 2012, 155, 2/4, pp 332-340
Abstract

To efficiently prevent and treat bovine mastitis and minimize its effect on the dairy industry, a sensitive, rapid, and specific test is required for identifying the mastitis-causing pathogens. In this study, a biochip capable of detecting 7 common species of mastitis-causing pathogens, including ...

Author(s)
Lee KuoHua; Lee JaiWei; Wang ShihWen; Liu LuYuan; Lee MeiFen; Chuang ShihTe; Shy YihMin; Chang ChuLi; Wu MingChe; Chi ChauHwa
Publisher
American Association of Veterinary Laboratory Diagnosticians, Davis, USA
Citation
Journal of Veterinary Diagnostic Investigation, 2008, 20, 4, pp 463-471

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