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Abstract

In this study, we isolated and analysed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The...

Author(s)
Vercauteren, I.; Maere, V. de; Vercruysse, J.; Stevens, M.; Gevaert, K.; Claerebout, E.
Publisher
American Society of Parasitologists, Lawrence, USA
Citation
Journal of Parasitology, 2006, 92, 6, pp 1244-1250
Abstract

Intramuscular immunization of calves with an excretory-secretory antigen fraction enriched for cysteine proteinase activity (ES-thiol) and QuilA as adjuvant induces a protective immune response against the abomasal nematode Ostertagia ostertagi. A study was conducted to confirm the protective...

Author(s)
Geldhof, P.; Vercauteren, I.; Vercruysse, J.; Knox, D. P.; Broeck, W. van den; Claerebout, E.
Publisher
Blackwell Publishing, Oxford, UK
Citation
Parasite Immunology, 2004, 26, 1, pp 37-43
Abstract

The identification of protective helminth antigens remains the most important challenge in the development of parasitic vaccines. To identify protective antigens of Ostertagia ostertagi, an important abomasal parasite of cattle, parasite-specific local antibodies from the abomasal mucus and from...

Author(s)
Maere, V. de; Vercauteren, I.; Saverwyns, H.; Claerebout, E.; Berx, G.; Vercruysse, J.
Publisher
Cambridge University Press, Cambridge, UK
Citation
Parasitology, 2002, 125, 4, pp 383-391
Abstract

Previous vaccination trials against Ostertagia ostertagi in cattle have demonstrated the protective capacity of a protein fraction termed ES-thiol, which is enriched for activation-associated secreted proteins (ASPs) and cysteine proteases. In this study, ES-thiol was subfractionated through...

Author(s)
Meyvis, Y.; Geldhof, P.; Gevaert, K.; Timmerman, E.; Vercruysse, J.; Claerebout, E.
Publisher
Elsevier, Amsterdam, Netherlands
Citation
Veterinary Parasitology, 2007, 149, 3/4, pp 239-245
Abstract

As an alternative to antihelminthic drugs, we are exploiting vaccination to control infections with the abomasal nematode Ostertagia ostertagi in cattle. Our focus for vaccine targets is excretory-secretory (ES) products of this parasite. One of the most abundant antigens in larval and adult ...

Author(s)
Vercauteren, I.; Geldhof, P.; Vercruysse, J.; Peelaers, I.; Broeck, W. van den; Gevaert, K.; Claerebout, E.
Publisher
American Society for Microbiology (ASM), Washington, USA
Citation
Infection and Immunity, 2004, 72, 5, pp 2995-3001
Abstract

Protease inhibitors are thought to protect intestinal parasitic nematodes from their hostile proteolytic environment. In a previous study, screening of Ostertagia ostertagi cDNA libraries with local antibody probes of the abomasal lymph nodes and mucus revealed a (28 kDa) aspartyl protease...

Author(s)
Maere, V. de; Vercauteren, I.; Gevaert, K.; Vercruysse, J.; Claerebout, E.
Publisher
Elsevier, Oxford, UK
Citation
Molecular and Biochemical Parasitology, 2005, 141, 1, pp 81-88
Abstract

The protective capacity of an adult stage Ostertagia ostertagi globin antigen was tested in four vaccination experiments in cattle. In a preliminary experiment, calves were vaccinated three times intraperitoneally with 250 µg globin in Freund's adjuvant and challenged with a trickled infection of...

Author(s)
Claerebout, E.; Smith, W. D.; Pettit, D.; Geldhof, P.; Raes, S.; Geurden, T.; Vercruysse, J.
Publisher
Elsevier, Amsterdam, Netherlands
Citation
Veterinary Parasitology, 2005, 128, 3/4, pp 299-307
Abstract

Previous vaccination trials against the economically important cattle parasite Ostertagia ostertagi have indicated the protective capacity of activation-associated secreted proteins (ASPs). The further development of these antigens into a commercial vaccine will require their recombinant...

Author(s)
Geldhof, P.; Meyvis, Y.; Vercruysse, J.; Claerebout, E.
Publisher
Blackwell Publishing, Oxford, UK
Citation
Parasite Immunology, 2008, 30, 1, pp 57-60
Abstract

The relative contribution of the 3rd (L3), 4th (L4) and adult stages of O. ostertagi to the development of immunity was assessed in calves which were either continuously infected during 21 weeks or subjected to infections truncated by anthelmintic treatment at the L3 or L4 stage. A 4th group...

Author(s)
Claerebout, E.; Hilderson, H.; Meeus, P.; Marez, T. de; Behnke, J.; Huntley, J.; Vercruysse, J.
Citation
Veterinary Parasitology, 1996, 66, 3/4, pp 225-239
Abstract

Excretory-secretory and somatic antigens of Ostertagia ostertagi fourth stage larvae, emulsified with complete Freund's adjuvant, were ip administered to calves on 3 occasions. Two weeks after the last immunisation all calves were infected with a single dose of 130 000 O. ostertagi 3rd stage...

Author(s)
Hilderson, H.; Berghen, P.; Graaf, D. C. de; Claerebout, E.; Vercruysse, J.
Citation
International Journal for Parasitology, 1995, 25, 6, pp 757-760

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