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Abstract

In this study, we isolated and analysed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The...

Author(s)
Vercauteren, I.; Maere, V. de; Vercruysse, J.; Stevens, M.; Gevaert, K.; Claerebout, E.
Publisher
American Society of Parasitologists, Lawrence, USA
Citation
Journal of Parasitology, 2006, 92, 6, pp 1244-1250
Abstract

Intramuscular immunization of calves with an excretory-secretory antigen fraction enriched for cysteine proteinase activity (ES-thiol) and QuilA as adjuvant induces a protective immune response against the abomasal nematode Ostertagia ostertagi. A study was conducted to confirm the protective...

Author(s)
Geldhof, P.; Vercauteren, I.; Vercruysse, J.; Knox, D. P.; Broeck, W. van den; Claerebout, E.
Publisher
Blackwell Publishing, Oxford, UK
Citation
Parasite Immunology, 2004, 26, 1, pp 37-43
Abstract

The identification of protective helminth antigens remains the most important challenge in the development of parasitic vaccines. To identify protective antigens of Ostertagia ostertagi, an important abomasal parasite of cattle, parasite-specific local antibodies from the abomasal mucus and from...

Author(s)
Maere, V. de; Vercauteren, I.; Saverwyns, H.; Claerebout, E.; Berx, G.; Vercruysse, J.
Publisher
Cambridge University Press, Cambridge, UK
Citation
Parasitology, 2002, 125, 4, pp 383-391
Abstract

As an alternative to antihelminthic drugs, we are exploiting vaccination to control infections with the abomasal nematode Ostertagia ostertagi in cattle. Our focus for vaccine targets is excretory-secretory (ES) products of this parasite. One of the most abundant antigens in larval and adult ...

Author(s)
Vercauteren, I.; Geldhof, P.; Vercruysse, J.; Peelaers, I.; Broeck, W. van den; Gevaert, K.; Claerebout, E.
Publisher
American Society for Microbiology (ASM), Washington, USA
Citation
Infection and Immunity, 2004, 72, 5, pp 2995-3001
Abstract

Protease inhibitors are thought to protect intestinal parasitic nematodes from their hostile proteolytic environment. In a previous study, screening of Ostertagia ostertagi cDNA libraries with local antibody probes of the abomasal lymph nodes and mucus revealed a (28 kDa) aspartyl protease...

Author(s)
Maere, V. de; Vercauteren, I.; Gevaert, K.; Vercruysse, J.; Claerebout, E.
Publisher
Elsevier, Oxford, UK
Citation
Molecular and Biochemical Parasitology, 2005, 141, 1, pp 81-88
Abstract

The objective of this study was to evaluate abomasal cytokine responses in helminth-naive calves and calves vaccinated with protective antigen fractions from Ostertagia ostertagi after an experimental challenge infection with infective third stage (L3) larvae. Abomasal lymph nodes and/or abomasal...

Author(s)
Claerebout, E.; Vercauteren, I.; Geldhof, P.; Olbrechts, A.; Zarlenga, D. S.; Goddeeris, B. M.; Vercruysse, J.
Publisher
Blackwell Publishing, Oxford, UK
Citation
Parasite Immunology, 2005, 27, 9, pp 325-331
Abstract

Cysteine proteinase enriched fractions obtained by thiol-sepharose chromatography of O. ostertagi membrane-bound protein extract (S3-thiol) or total adult excretory-secretory (ES-thiol) products were tested in a vaccination experiment to evaluate their protective efficacy against O. ostertagi in ...

Author(s)
Geldhof, P.; Claerebout, E.; Knox, D.; Vercauteren, I.; Looszova, A.; Vercruysse, J.
Publisher
Blackwell Science, Oxford, UK
Citation
Parasite Immunology, 2002, 24, 5, pp 263-270