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Abstract

Objective: The objective of this study was to construct and express the TAT-bSox2-9R fusion vector, which would improve the formation of safe induced pluripotent stem cells using TAT and Poly-Arg (9R) peptides. Method: According to the available coding region of bovine Sox2 gene in GenBank...

Author(s)
Jia WenChao; Gan Peng; Xiong Liang; Pan ChuanYing; Lan XianYong; Chen Hong
Publisher
Northwest A & F University (Natural Science Edition), Yangling, China
Citation
Journal of Northwest A & F University - Natural Science Edition, 2013, 41, 11, pp 1-6
AbstractFull Text

Through shot-gun method, one hundred and three DNA segments of promoter from genomic library of 168 Bacillus subtilis were detected with Escherichia coli-B. subtilis shuttle plasmid. The activities of the reporter gene of bgaB driven by one promoter fragment, P3.4.23, exhibited high expression...

Author(s)
Zhang AiLing; Zhang Li; Yang MingMing; Zhang LiangZhi; Chen Hong
Publisher
South China Agricultural University, Guangzhou, China
Citation
Journal of South China Agricultural University, 2011, 32, 3, pp 92-96
Abstract

A 1 278 bp coding sequences of hypocretin receptor 1 (HCRTR1) gene (GenBank accession No.: DQ874350) in Qinchuan cattle (Bos tarus) was obtained by the Overlap-PCR methods and cloned into pMD18-T vector, and the positive clone plasmid (pMD18T-HCRTR1) was sequenced. Results showed that the cloned...

Author(s)
Zhang Li; Zhang LiangZhi; Zhang AiLing; Chen Hong; Zhang ChunLei; Zhang Qin
Publisher
China Agricultural University, Beijing, China
Citation
Journal of Agricultural Biotechnology, 2008, 16, 4, pp 568-572