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Abstract

In this study, we detected the polymorphism of SNPs of intron 6 in CAPN4 gene in Datong Yak, Tianzhu White Yak, Gannan Yak and cattle by PCR-SSCP and DNA sequencing in order to understand the genetic characters of this gene in yak and cattle. The results showed that the 6 intron of CAPN4 gene in...

Author(s)
Zhang Li; Liu LiXia; Chen Hong; Yu LingXiao
Publisher
Guangxi University, Guangxi, China
Citation
Genomics and Applied Biology, 2017, 36, 12, pp 5099-5103
Abstract

To search the molecular markers that are associated with mastitis and to provide a theoretical basis for the Holstein cattle breeding and disease resistance, the polymorphisms of Nramp1 gene in 303 Holstein cattle in Ningxia were detected by the PCR-SSCP technique and the effect of Nramp1 gene...

Author(s)
Zhang Li; Liu LiXia; Chen Hong; Li QiangZi; Liu WuXin
Publisher
Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Citation
Acta Agriculturae Zhejiangensis, 2017, 29, 3, pp 389-394
Abstract

The Pit-1 gene was studied as a candidate for genetic markers of growth traits in Chinese cattle in this study. The single-strand conformation polymorphism method was used to identify polymorphism in the Pit-1 intron 5. One single nucleotide polymorphism, Pit1I5, was found in intron 5. The...

Author(s)
Tang LiGang; Yang DongYing; Ouyang WuQing; Zhang LiangZhi; Lan XianYong; Zhang ChunLei; Zhang RunFeng; Zhang AiLing; Zhang Li; Chen Hong
Publisher
Academic Journals, Nairobi, Kenya
Citation
African Journal of Biotechnology, 2012, 11, 42, pp 9906-9910
Abstract

Ghrelin is an important peptide that stimulates food intake and regulates energy balance of animals. Single nucleotide polymorphisms of ghrelin gene in three Chinese cattle populations were investigated through PCR-SSCP and DNA sequencing. Five over-lapped DNA fragments were analyzed and a total of ...

Author(s)
Zhang AiLing; Zhang Li; Zhang LiangZhi; Zhang CunFang; Lan XianYong; Zhang ChunLei; Chen Hong
Publisher
Springer, Amsterdam, Netherlands
Citation
Molecular Biology Reports, 2012, 39, 6, pp 6981-6986
Abstract

Neuropeptide Y (NPY) is one of the most potent orexigenic factors that is implicated in the control of feeding behavior and energy homeostasis. Four Chinese indigenous cattle breeds (n=338) were used to detect single nucleotide polymorphisms (SNPs) in the coding regions with their intron-exon...

Author(s)
Zhang Li; Zhang AiLing; Zhang LiangZhi; Zhang Qin; Zhang ChunLei; Lan XianYong; Chen Hong
Publisher
Elsevier, Amsterdam, Netherlands
Citation
General and Comparative Endocrinology, 2011, 170, 1, pp 189-192
AbstractFull Text

Through shot-gun method, one hundred and three DNA segments of promoter from genomic library of 168 Bacillus subtilis were detected with Escherichia coli-B. subtilis shuttle plasmid. The activities of the reporter gene of bgaB driven by one promoter fragment, P3.4.23, exhibited high expression...

Author(s)
Zhang AiLing; Zhang Li; Yang MingMing; Zhang LiangZhi; Chen Hong
Publisher
South China Agricultural University, Guangzhou, China
Citation
Journal of South China Agricultural University, 2011, 32, 3, pp 92-96
Abstract

In this study, overlap-PCR, an efficient and rapid method, was used to clone cattle Ghrelin gene CDS (coding sequence) from genomic DNA. The procedure included seven primers and three-step PCRs. Cattle Ghrelin gene consists of four exons and the CDS contains 351 bps. In the first step three PCRs...

Author(s)
Zhang AiLing; Zhang Li; Zhang LiangZhi; Chen Hong; Lan XianYong; Zhang ChunLei; Zhang CunFang
Publisher
Elsevier Inc., San Diego, USA
Citation
Biochemical and Biophysical Research Communications, 2010, 391, 3, pp 1490-1493
Abstract

The AMP-activated protein kinase (AMPK) is an evolutionarily conserved sensor of cellular and systemic energy balance. PRKAB1, the gene that encodes the β1 regulatory subunit of AMPK, has been shown to be highly involved in the glycogen metabolism. To date, several mutations affecting function of...

Author(s)
Zhang Qin; Chen Hong; Zhao Sheng; Zhang Li; Zhang LiangZhi; Li Fei; Wang XueMing
Publisher
Humana Press, Totowa, USA
Citation
Molecular Biotechnology, 2009, 43, 3, pp 193-199
Abstract

ACRP30 gene was located nearby the QTL affecting the marbling, ribeye muscle area and fat thickness on the BAT1 in Angus. In this study, a 5bp deletion mutation within the bovine ACRP30 gene was firstly detected and confirmed in 991 cattle by PCR-SSCP, DNA sequencing and direct PCR amplification....

Author(s)
Zhang LiangZhi; Chen Hong; Lan XianYong; Zhang CunFang; Zhang Li; Zhang AiLing; Zhang Qin; Lei CunLei; Zhang HanYang
Publisher
Springer, Dordrecht, Netherlands
Citation
Molecular Biology Reports, 2009, 36, 5, pp 895-899
Abstract

This study was conducted to analyse the single nucleotide polymorphisms (SNPs) in hypocretin receptor 1 (HCRTR1) gene of Nanyang cattle. 122 Nanyang cattle were used for SNPs analysis in a complete coding region of HCRTR1 gene using PCR-SSCP and sequencing methods. Haplotypes and genotypes were...

Author(s)
Zhang Li; Zhang AiLing; Zhang LiangZhi; Zhang ChunLei; Zhu ZeYi; Lei ChuZhao; Wang XinZhuang; Chen Hong
Publisher
Editorial Department of Scientia Agricultura Sinica, Beijing, China
Citation
Scientia Agricultura Sinica, 2008, 41, 5, pp 1560-1566

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