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Abstract

A strategy for the amplification and subsequent cloning of mRNA or cDNA from Dictyocaulus viviparus L4 and L5 was developed, and the in vitro cultivation of larvae was established and optimized. The technique was developed using L3, which are more readily available. The average total RNA content of ...

Author(s)
Samson-Himmelstjerna, G. von
Publisher
Tierärztliche Hochschule Hannover, Hannover, Germany
Citation
PCR-gestützte Konstruktion von cDNA-Banken aus kleinsten Mengen von Dictyocaulus viviparus-Larven., 1995, pp 148 pp.
Abstract

Two strategic programs for the control of gastrointestinal nematodes were compared in a herd of 24 first-year grazing cattle. After allocating the animals to 3 groups of equal size, one group was treated with ivermectin pour-on (1ml per 10 kg body weight) at turnout and 6 weeks later, a 2nd group...

Author(s)
Schnieder, T.; Epe, C.; Samson-Himmelstjerna, G. von; Kohlmetz, C.; Woidtke, S.
Citation
Praktische Tierarzt, 1996, 77, 6, pp 529-536
Abstract

Two groups of 8 first-year grazing cattle were either left untreated as controls (group 1) or each animal was treated at turnout with an ivermectin bolus containing 1.72 g ivermectin and designed to deliver 12 mg/day for 135 days (group 2). Whereas group 1 suffered from lungworm infection (...

Author(s)
Schnieder, T.; Epe, C.; Samson-Himmelstjerna, G. von; Kohlmetz, C.
Citation
Applied Parasitology, 1996, 37, 1, pp 38-44
Abstract

Interactions between treatment with an ivermectin bolus (designed to release 12 mg/day over 135 days) at turnout and immunity to bovine parasitic gastroenteritis and bronchitis were examined. Immunity-related parameters, i.e. development of clinical disease, parasite development and stimulation of...

Author(s)
Schnieder, T.; Epe, C.; Samson-Himmelstjerna, G. von; Kohlmetz, C.
Citation
Veterinary Parasitology, 1996, 64, 3, pp 239-250
Abstract

In a comparative study of the lungworm species D. viviparus and D. eckerti (which is not universally accepted as a separate species), the restriction fragment length polymorphism (RFLP) of the PCR amplified ribosomal second internal transcribed spacer (ITS2) and their sequences were examined....

Author(s)
Epe, C.; Samson-Himmelstjerna, G. von; Stoye, M.; Schnieder, T.
Citation
Berliner und Münchener Tierärztliche Wochenschrift, 1996, 109, 6/7, pp 227-231
Abstract

A strategy is described for the amplification and cloning of cDNA from minute amounts of Dictyocaulus viviparus larvae. Initially, L3 were used to establish the procedure. Amplification of cDNA synthesized from approximately 400 ng total RNA from 5000 L3 generated products that were more than 800...

Author(s)
Samson-Himmelstjerna, G. von; Wunderlich, G.; Mühlschlegel, F.; Frosch, M.; Schnieder, T.
Citation
Parasitology Research, 1997, 83, 1, pp 20-23
Abstract

Using substantial interspecific differences between the 2nd internal transcribed spacer (ITS2) region within the rDNA gene of Dictyocaulus eckerti and D. viviparus a species-specific PCR was developed to distinguish between lungworm larvae of the 2 species from fallow deer and cattle. It was found...

Author(s)
Samson-Himmelstjerna, G. von; Woidtke, S.; Epe, C.; Schnieder, T.
Citation
Veterinary Parasitology, 1997, 68, 1/2, pp 119-126
Abstract

Exsheathed Dictyocaulus viviparus larvae obtained from experimentally infected calves were cultivated in vitro in RPMI-1640 medium (pH 7.2) containing 20% newborn calf serum, 200 U/ml Moronal, and 200 U/ml penicillin/streptomycin. Incubation was performed at 39.5°C and 0, 5, 10, and 20% CO2....

Author(s)
Samson-Himmelstjerna, G. von; Rickling, S.; Schnieder, T.
Citation
Parasitology Research, 1998, 84, 8, pp 682-684
Abstract

Some morphological features of inhibited L4 and 4th moult larvae (4M) of Dictyocaulus viviparus are described. Inhibition was induced by maintaining the L3 for 6 weeks at 4°C. Inhibited L4 and 4M were collected by perfusion of the lungs of experimentally-infected calves after necropsy at 15 and 68...

Author(s)
Samson-Himmelstjerna, G. von; Schnieder, T.
Citation
Journal of Helminthology, 1999, 73, 1, pp 79-83
Abstract

PF 1022A is a member of a new class of cyclic depsipeptides with antiparasitic activity. Following in vitro and laboratory animal studies, it was tested for its anthelmintic efficacy in companion and livestock animals against a wide spectrum of intestinal nematodes and lungworms. Studies were...

Author(s)
Samson-Himmelstjerna, G. von; Harder, A.; Schnieder, T.; Kalbe, J.; Mencke, N.
Citation
Parasitology Research, 2000, 86, 3, pp 194-199

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