We expressed a catalytically active cysteine protease, Ac-CP-2 (spanning Ala-12 to the C terminal Val-340; GenBank accession number U18912), from the blood-feeding stage of the canine hookworm Ancylostoma caninum and vaccinated dogs with the purified protease. Dogs acquired high-titre,...

Intestinal spirochetosis and its causal agent Brachyspira pilosicoli are known to cause production losses especially in pigs and own fattening phase. The disease is chiefly characterized by a milder course without significant mortality, with the occurrence of diarrhea, characteristically without...

Ligand binding activity of intrinsic factor-cobalamin receptor (IFCR) was determined in homogenates and isolated brush-border membranes (BBM) of ileum and kidney from dogs with signs of simple autosomal recessive inheritance of selective cobalamin malabsorption. IFCR activity of affected dog ileal...

To comprehend the renal defect underlying the idiopathic Fanconi syndrome in the Basenji dog, isolated renal brush border membrane vesicles were used to examine two factors that influence membrane nonelectrolyte transport processes, sodium flux and membrane fluidity. There was no significant...

Mucosal biopsies from canine jejunum and ileum were established in organ culture and remained structurally intact for up to 72 hours. Infection of such mucosal biopsies with an enteropathogenic Escherichia coli (EPEC) 0111 results in the characteristic loss of brush border observed during natural...

Total phospholipid of the isolated renal brush border membranes from 4 Basenji dogs with Fanconi syndrome analysed by ³¹P nuclear magnetic resonance showed no difference compared with that of normal dogs. Examination of total fatty acids in both membranes using gas-liquid chromatography analysis of ...

Two animal models for cystinuria have been examined: the Basenji dog with Fanconi syndrome and cystine stone-forming dogs of different breeds. Brush-border membranes were isolated from the dogs and uptake of smallcap˜D-glucose and smallcap˜L-cystine was studied. Experiments with isolated brush-...

Chicks were challenged with North American S. pilosicoli, a weakly β-haemolytic intestinal spirochaete (WBHIS). Chicks in control groups received trypticase soy broth or canine Serpulina innocens. The birds were killed at weekly intervals. No WBHIS were isolated from the caeca of controls, but...

Dogs with naturally occurring aerobic or anaerobic bacterial overgrowth were examined before and after antibiotic therapy in order to assess reversibility of damage to the jejunal mucosa. Histological changes in peroral jejunal biopsies were relatively minor before and after treatment, but sucrose...

Biosynthesis of aminopeptidase N (ApN), alkaline phosphatase (AP) and total microvillus membrane protein was studied in a litter of 6 Irish Setters with gluten-sensitive enteropathy (GSE) and 3 healthy Greyhounds. Explants obtained from affected dogs at 4 and 12 months were maintained in vitro and...