Enucleation of a recipient oocyte is one of the key processes in the procedure of somatic cell nuclear transfer (SCNT). However, especially in bovine species, lipid droplets spreading in the ooplasm hamper identification and enucleation of metaphase II (MII) chromosomes, and thereby the success...

As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ...

Enucleation of a recipient oocyte is an important essential process in the procedure of somatic cell nuclear transfer (SCNT). The present study investigated a method for the improvement of enucleation efficiency. Oocytes were denuded of cumulus cells before the completion of nuclear maturation...

The purpose of this study was to optimize the manipulation of interspecies somatic cell nuclear transfer (ISCNT) between Sika deer and bovine. It was used the Sika deer ear skin fibroblast as nuclear donor cell, and bovine MII oocyte as recipient. McGrath-Solter, extrusion and DEME chemically...

This paper describes a case study of irreversibly damaged eyeball of two-year old Holstein cow and his surgical removal by transpalpebral enucleation. The operation was successfully performed.

Infectious keratoconjunctivitis (pinkeye) is a common problem in sheep and goats. Several etiologies as well as diagnostic techniques, treatment, and control are considered. Entropion is an important differential diagnosis, especially in young animals with eye irritation, and several inexpensive...

The aim of this study was to investigate the application of demecolcine assisted oocyte enucleation in bovine somatic cell nuclear transfer (SCNT) and the development ability of embryos produced by this technique. After 19 h maturation, the chromosomes of bovine MIl oocytes were eliminated by...

Effects of enucleation timing on enucleation rates, development and methylation levels of reconstructed bovine embryos were investigated. However, the enucleation rate of reconstructed embryos produced by the enucleation before fusion and activation (EBFA) was higher than that by the enucleation...

Demecolcine-assisted/induced enucleation has been used in nuclear transfer cloning procedures for many species, yet its mechanism of action remains unclear. Primarily because oocytoplasm protrusion induced by demecolcine is inhibited by the presence of cytochalasin, its use has had limited...

This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic ...