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Abstract

Enucleation of a recipient oocyte is one of the key processes in the procedure of somatic cell nuclear transfer (SCNT). However, especially in bovine species, lipid droplets spreading in the ooplasm hamper identification and enucleation of metaphase II (MII) chromosomes, and thereby the success...

Author(s)
Iwamoto, D.; Yamagata, K.; Kishi, M.; Hayashi-Takanaka, Y.; Kimura, H.; Wakayama, T.; Saeki, K.
Publisher
Mary Ann Liebert, Inc., New Rochelle, USA
Citation
Cellular Reprogramming, 2015, 17, 2, pp 106-114
Abstract

As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ...

Author(s)
Saraiva, N. Z.; Oliveira, C. S.; Leal, C. L. V.; Lima, M. R. de; Collado, M. del; Vantini, R.; Monteiro, F. M.; Niciura, S. C. M.; Garcia, J. M.
Publisher
Cambridge University Press, Cambridge, UK
Citation
Zygote, 2015, 23, 6, pp 852-862
Abstract

Somatic cell nuclear transfer (SCNT) allows animal cloning but remains technically challenging. This study investigated limitations to functional oocyte enucleation by actinomycin D (AD) as a means of making SCNT easier to perform. Denuding oocytes or inhibiting transcription before AD treatment...

Author(s)
Moura, M. T.; Badaraco, J.; Sousa, R. V.; Lucci, C. M.; Rumpf, R.
Publisher
CSIRO, Collingwood, Australia
Citation
Reproduction, Fertility and Development, 2019, 31, 8, pp 1321-1329
Abstract

This paper describes a case study of irreversibly damaged eyeball of two-year old Holstein cow and his surgical removal by transpalpebral enucleation. The operation was successfully performed.

Author(s)
Tejnil, I.; Pleško, M.
Publisher
Profi Press, s.r.o., Praha 2, Czech Republic
Citation
Veterinářství, 2017, 67, 1, pp 43-45
Abstract

Enucleation of a recipient oocyte is an important essential process in the procedure of somatic cell nuclear transfer (SCNT). The present study investigated a method for the improvement of enucleation efficiency. Oocytes were denuded of cumulus cells before the completion of nuclear maturation...

Author(s)
Jeon, B. G.; Betts, D. H.; King, W. A.; Rho, G. J.
Publisher
Wiley-Blackwell, Berlin, Germany
Citation
Reproduction in Domestic Animals, 2011, 46, 6, pp 1035-1042
Abstract

The purpose of this study was to optimize the manipulation of interspecies somatic cell nuclear transfer (ISCNT) between Sika deer and bovine. It was used the Sika deer ear skin fibroblast as nuclear donor cell, and bovine MII oocyte as recipient. McGrath-Solter, extrusion and DEME chemically...

Author(s)
Wang ShiYong; Zheng JunJun; Yang YueChun; Liu ZongYue; Yu Miao; Yang FuHe
Publisher
Guoji Shudian (Institute of Scientific and Technological Information of China, China Publications Centre), Beijing, China
Citation
Acta Veterinaria et Zootechnica Sinica, 2014, 45, 8, pp 1381-1386
Abstract

This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic ...

Author(s)
Saraiva, N. Z.; Perecin, F.; Méo, S. C.; Ferreira, C. R.; Tetzner, T. A. D.; Garcia, J. M.
Publisher
Mary Ann Liebert, Inc., New Rochelle, USA
Citation
Cloning and Stem Cells, 2009, 11, 1, pp 141-152
Abstract

In order to enhance the efficiency of somatic cell nuclear transfer, M II oocytes of Yanbian cattle were treated by demecolcine. Nucleus of oocytes were deleted by micromanipulation. These oocytes were prepared for the nuclear transfer receptor. The effects of the concentration of demecolcine, the...

Author(s)
Wang YuHan; Zhu YuXia; Yin Duo; Li ZhongShu; Fang NanZhu
Publisher
Jiangxi Agricultural University, Nanchang, China
Citation
Acta Agriculturae Universitatis Jiangxiensis, 2011, 33, 2, pp 340-344
Abstract

The aim of this study is to produce cloned bovine embryos with the use of enucleated oocytes without the zona pellucida as cytoplasts and fetal fibroblasts as donors of nuceli, as well as to improve the stages of electrofusion and in vitro culturing of bovine embryos without the zona pellucida up...

Author(s)
Prokof'ev, M. I.; Komissarov, A. V.; Stepanov, O. I.; Antipova, T. A.; Pinyugina, M. V.; Malenko, G. P.
Publisher
Allerton Press, Inc., New York, USA
Citation
Russian Agricultural Sciences, 2008, 34, 5, pp 340-342
Abstract

The aim of this study was to investigate the application of demecolcine assisted oocyte enucleation in bovine somatic cell nuclear transfer (SCNT) and the development ability of embryos produced by this technique. After 19 h maturation, the chromosomes of bovine MIl oocytes were eliminated by...

Author(s)
Sha Wei; Guo ZhenHua; Liu He; Yin ZhiMing; Ma Hong; Li ZhongQiu; Wu SaiHui; Liu Di
Publisher
Editorial Office of China Biotechnology, Beijing, China
Citation
China Biotechnology, 2009, 29, 2, pp 87-91

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