Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

CABI Book Chapter

Bt resistance: characterization and strategies for GM crops producing Bacillus thuringiensis toxins.

Book cover for Bt resistance: characterization and strategies for GM crops producing <i xmlns="http://www.w3.org/1999/xhtml">Bacillus thuringiensis</i> toxins.

Description

This book focuses on descriptions of the extent of use of Bacillus thuringiensis (Bt) crops and the emerging problem of resistance, recent progress in elucidating the mechanism of action of Bt toxins and describing the different resistance mechanisms and strategies for coping with resistance in the field. There are four sections. In the first section ('The extent of use of Bt crops and the emergin...

Chapter 15 (Page no: 162)

RNA interference strategy for crop protection against insect pests.

RNA interference (RNAi) has significantly accelerated functional genomic research on insects. Since its discovery, numerous reports have reported efforts to apply RNAi approaches to insect species lacking characterized genomes. The technique also has substantial potential for use in the control of insect pests that cause severe loss of crop yield and quality. Several approaches have been exploited to control these pests, including the application of different agrochemicals and the development of resistant crops by breeding and transgenic approaches. However, there are certain limitations with these strategies, and therefore novel alternative strategies are required for the development of stress-tolerant plants. RNAi strategies have proven to be such a novel and potential alternative for disease and pest control. This technique essentially involves the production of hairpin double-stranded RNA (dsRNA) in transgenic plants that targets pest genes that are essential for their growth and development. This chapter provides an insight into the practical aspects of this gene silencing methodology for the development of tools for pest management.

Other chapters from this book

Chapter: 1 (Page no: 1) Successes and failures of transgenic Bt crops: global patterns of field-evolved resistance. Author(s): Tabashnik, B. E., Carrière, Y.
Chapter: 2 (Page no: 15) Status of resistance to Bt cotton in China: cotton bollworm and pink bollworm. Author(s): Gao YuLin, Liu ChenXi, Wu YiDong, Wu KongMing
Chapter: 3 (Page no: 26) Insect resistance to Bt toxins in Brazil and Latin America. Author(s): Monnerat, R., Martins, E., Queiroz, P., Praça, L., Soares, C. M.
Chapter: 4 (Page no: 36) Resistance of Busseola fusca to Cry1Ab Bt maize plants in South Africa and challenges to insect resistance management in Africa. Author(s): Berg, J. van den, Campagne, P.
Chapter: 5 (Page no: 49) Resistance of cabbage loopers to Btk in a greenhouse setting: occurrence, spread and management. Author(s): Janmaat, A., Franklin, M., Myers, J. H.
Chapter: 6 (Page no: 56) Different models of the mode of action of Bt 3d-Cry toxins. Author(s): Bravo, A., Gómez, I., Mendoza, G., Gaytán, M., Soberón, M.
Chapter: 7 (Page no: 69) Roles of insect midgut cadherin in Bt intoxication and resistance. Author(s): Fabrick, J. A., Wu YiDong
Chapter: 8 (Page no: 87) Mechanism of Cry1Ac resistance in cabbage loopers - a resistance mechanism selected in insect populations in an agricultural environment. Author(s): Wang, P.
Chapter: 9 (Page no: 98) Roles of ABC proteins in the mechanism and management of Bt resistance. Author(s): Heckel, D. G.
Chapter: 10 (Page no: 107) The role of proteolysis in the biological activity of Bt insecticidal crystal proteins. Author(s): Zalunin, I. A., Elpidina, E. N., Oppert, B.
Chapter: 11 (Page no: 119) The lessons that Caenorhabditis elegans has taught us about the mechanism of action of crystal proteins. Author(s): Sitaram, A., Aroian, R. V.
Chapter: 12 (Page no: 126) The development and prospect of discovery of Bt toxin genes. Author(s): Zhang Jie, Shu ChangLong, Wang ZeYu
Chapter: 13 (Page no: 138) Cry toxin binding site models and their use in strategies to delay resistance evolution. Author(s): Jakka, S., Ferré, J., Jurat-Fuentes, J. L.
Chapter: 14 (Page no: 150) Countering pest resistance with genetically modified Bt toxins. Author(s): Soberón, M., García-Gómez, B. I., Pacheco, S., Sánchez, J., Tabashnik, B. E., Bravo, A.
Chapter: 16 (Page no: 173) Resistance management for Bt maize and above-ground lepidopteran targets in the USA: from single gene to pyramided traits. Author(s): Huang, F. N.
Chapter: 17 (Page no: 186) Insect resistance management and integrated pest management for Bt crops: prospects for an area-wide view. Author(s): Hutchison, W. D.