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Datasheet

Banana bunchy top virus (bunchy top of banana)

Summary

  • Last modified
  • 27 March 2018
  • Datasheet Type(s)
  • Invasive Species
  • Pest
  • Preferred Scientific Name
  • Banana bunchy top virus
  • Preferred Common Name
  • bunchy top of banana
  • Taxonomic Tree
  • Domain: Virus
  •   Unknown: "ssDNA viruses"
  •     Unknown: "DNA viruses"
  •       Family: Nanoviridae
  •         Genus: Babuvirus

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Pictures

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PictureTitleCaptionCopyright
'Rosette' or 'bunchytop'. Appearance of BBTV-infected banana caused by the production of progressively shorter, narrower and more upright leaves.
TitleSymptoms on banana
Caption'Rosette' or 'bunchytop'. Appearance of BBTV-infected banana caused by the production of progressively shorter, narrower and more upright leaves.
CopyrightDavid Jones
'Rosette' or 'bunchytop'. Appearance of BBTV-infected banana caused by the production of progressively shorter, narrower and more upright leaves.
Symptoms on banana'Rosette' or 'bunchytop'. Appearance of BBTV-infected banana caused by the production of progressively shorter, narrower and more upright leaves.David Jones
Yellowing and curling of leaf margins on BBTV-infected banana.
TitleSymptoms on banana
CaptionYellowing and curling of leaf margins on BBTV-infected banana.
CopyrightDavid Jones
Yellowing and curling of leaf margins on BBTV-infected banana.
Symptoms on bananaYellowing and curling of leaf margins on BBTV-infected banana.David Jones
Yellowing and curling of leaf margins on BBTV-infected banana.
TitleSymptoms on banana - detail view
CaptionYellowing and curling of leaf margins on BBTV-infected banana.
CopyrightDavid Jones
Yellowing and curling of leaf margins on BBTV-infected banana.
Symptoms on banana - detail viewYellowing and curling of leaf margins on BBTV-infected banana.David Jones
Stunted plants arising from infected suckers.
TitleSymptoms on banana
CaptionStunted plants arising from infected suckers.
CopyrightDavid Jones
Stunted plants arising from infected suckers.
Symptoms on bananaStunted plants arising from infected suckers.David Jones
Dark green 'dot-dash' patterns in minor leaf veins forming 'hooks' where they enter the edge of the midrib.
TitleSymptoms on leaf (detail view)
CaptionDark green 'dot-dash' patterns in minor leaf veins forming 'hooks' where they enter the edge of the midrib.
CopyrightDavid Jones
Dark green 'dot-dash' patterns in minor leaf veins forming 'hooks' where they enter the edge of the midrib.
Symptoms on leaf (detail view)Dark green 'dot-dash' patterns in minor leaf veins forming 'hooks' where they enter the edge of the midrib.David Jones

Identity

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Preferred Scientific Name

  • Banana bunchy top virus

Preferred Common Name

  • bunchy top of banana

Other Scientific Names

  • abacá bunchy top virus
  • banana bunchy top nanavirus

International Common Names

  • English: cabbage top of banana; curly top of banana
  • Spanish: cogollo racimoso del banano
  • French: maladie du bunchy top du bananier; sommet touffu du bananier

English acronym

  • BBTV

EPPO code

  • BBTV00 (Banana bunchy top nanovirus)

Taxonomic Tree

Top of page
  • Domain: Virus
  •     Unknown: "ssDNA viruses"
  •         Unknown: "DNA viruses"
  •             Family: Nanoviridae
  •                 Genus: Babuvirus
  •                     Species: Banana bunchy top virus

Notes on Taxonomy and Nomenclature

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Banana bunchy top virus (BBTV) was originally included in the luteovirus group, but is now recognised as a species of the Nanovirus genus (van Regenmortel et al., 2000), one of two genera included in the recently recognised Nanoviridae family.

Description

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Evidence for a Viral Agent

BBTV is presumed to be the causal agent of bunchy top of banana, though unequivocal evidence, by reproduction of the disease through inoculation of purified virions or cloned genomic components, is lacking. The virions are intimately associated with the disease (Harding et al., 1991; Thomas and Dietzgen, 1991) and have been detected in all symptomatic plants tested (Dietzgen and Thomas, 1991; Thomas, 1991; Thomas and Dietzgen, 1991; Karan et al., 1994). Dale et al. (1986) isolated disease-specific dsRNA, suggestive of luteovirus infection, from Cavendish cultivars and Iskra-Caruana isolated similar dsRNAs from five of six bunchy top samples (ML Iskra-Caruana, Montpellier, 1997, personal communication). However, neither these, nor any subsequent studies, have identified or established a clear role for any virus other than the ssDNA BBTV in banana bunchy top disease.

Particle and Genome Properties

The virions of BBTV are icosahedra, ca 18-20 nm in diameter, have a coat protein of ca 20,000 Mr, a sedimentation coefficient of ca 46S and a buoyant density of 1.29-1.30 g/cm³ in caesium sulphate (Wu and Su, 1990c; Dietzgen and Thomas, 1991; Harding et al., 1991; Thomas and Dietzgen, 1991). Purified preparations have an A<(sub)260/280> of 1.33 (Thomas and Dietzgen, 1991). The virus possesses a multi-component genome, consisting of at least six circular ssDNA components each ca 1000-1100 nucleotides long (Wu et al., 1994; Yeh et al., 1994; Burns et al., 1995; Xie and Hu, 1995). Component 1 encodes two proteins and components 2-6 each encode one protein (Burns et al., 1995; Dale, 1996; Beetham et al., 1997). Two areas of the non-coding regions are highly conserved between the six components (Burns et al., 1995). The first is a stem-loop common region of up to 69 nucleotides. It contains a nonanucleotide loop sequence conserved amongst ssDNA plant viruses and which may be involved in rolling circle replication and initiation of viral strand DNA synthesis. The second, 5' to the stem-loop common region, is a major common region varying in size between components from 65 to 92 nucleotides and which may have a promoter function. The initiation factor for endogenous DNA primers is also located within the major common region (Hafner et al., 1997a). Component 1 encodes a putative replication initiation protein and contains a second functional open reading frame internal to this, whilst component 3 codes for the coat protein (Harding et al., 1993; Dale, 1996; Hafner et al., 1997b, Wanitchakorn et al., 1997). Component 5 has been shown to produce a gene product containing an LXCXE motif and to have retinoblastoma protein (Rb)-binding activity. The gene product may be produced very early in the infection cycle and be responsible for switching the first infected cells to S-phase in preparation for virus replication. Component 6 appears to encode nuclear shuttle proteins. The functions of the gene products of Component 2 and the small internal gene of component 1 are unknown (Wanitchakorn et al., 2000). Recent research indicates that component 1 is the minimal replicative unit of BBTV and encodes the 'master' viral Rep (Horser et al., 2001).

Strains of BBTV

Most isolates of BBTV are associated with typical severe disease symptoms. However, mild and symptomless isolates have been reported from Taiwan (Su et al., 1993) and may occur more widely, though apparently not in Australia (JE Thomas and ADW Geering, Brisbane, 1998, personal communication). BBTV has been confirmed in specimens of mild and symptomless infections from Taiwan by both ELISA and PCR (HJ Su, JL Dale and JE Thomas, Brisbane, personal communication, 1996) and the isolates can be transmitted by Pentalonia nigronervosa (HJ Su, Taipei, personal communication, 1996). Genomic differences, which correlate with these biological variants, have not yet been determined.

Two broad groups of isolates have been identified based on nucleotide sequence differences between some, possibly all, of the six recognized genome components (Karan et al., 1994; JL Dale, Brisbane, personal communication, 1996). The 'South Pacific' group comprises isolates from Australia, Fiji, Western Samoa, Tonga, Burundi, Egypt and India, whilst the 'Asian' group comprises isolates from the Philippines, Taiwan and Vietnam. These differences are present throughout the genomes of components 1 and 6, but are most striking in the untranslated major common region. No biological differences have been associated with these sequence differences.

Magee (1948) noted that certain plants of 'Veimama', a cultivar originally from Fiji and growing then in New South Wales, showed a 'partial recovery' from bunchy top symptoms and produced bunches. After an initial flush of typical severe symptoms in three or four leaves, subsequent leaves showed few, if any, dark-green flecks. Suckers derived from these partially recovered plants also displayed a flush of typical symptoms followed by partial recovery. The origin of the infection, whether from Australia or Fiji, was uncertain. This partial recovery was noted for some infected plants of 'Veimama' only, and in Fiji was noted for one sucker only on a single infected stool from among hundreds of infected stools of 'Veimama' observed. Magee was not able to transmit the virus from partially recovered plants and was only able to super-infect them, with difficulty, with high inoculum pressure. This may be an example of a mild strain of BBTV, possibly a non-aphid transmitted one, propagated vegetatively, reaching only a low titre and conferring a degree of cross-protection. Alternatively, 'Veimama' may not be uniform and individual plants with a degree of resistance may exist. The complete explanation for this phenomenon is unclear.

The inability to transmit bunchy top from abacá to banana (Ocfemia and Buhay, 1934) was originally considered evidence that two distinct strains of the virus existed. However, as noted by Magee (1953), technical deficiencies with these experiments mean that the results must be viewed with caution. As first suggested by Magee (1953), the many similarities between the bunchy top diseases of banana and abacá, including transmission properties with the vector P. nigronervosa, suggests that both are caused by the same virus. ELISA has recently detected BBTV in a sample of abacá bunchy top from the Philippines (JE Thomas and N Bajet, Brisbane and Los Baños, personal communication, 1993).

Distribution

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Reports of BBTV in Cambodia (Stover, 1972), French Polynesia (CMI, 1977), Laos (Stover, 1972), Hong Kong (Buddenhagen, 1968; CMI, 1977), Papua New Guinea (Magee, 1954; CMI, 1977), Sabah (Magee, 1927; CMI, 1977) and the United Arab Republic (Buddenhagen, 1968) remain unconfirmed (Dale, 1987; Thomas and Iskra-Caruana, 1999). Sulyo and Muharam (1985) reported BBTV to be present in Irian Jaya, Indonesia, but symptoms have not been seen in recent surveys (Shivas et al. ,1996; R Davis, Mareeba, personal communication, 2000). Although there have been reports of bunchy top disease in Peninsular Malaysia (CMI, 1977), a survey conducted by MARDI and INIBAP in 1993 failed to locate any plants with typical or atypical symptoms (DR Jones, Montpellier, personal communication, 1993). A report of bunchy top in South Africa in the early 1990s (Su et al., 1992) was found to be erroneous (Pietersen et al., 1996), but there us now a confirmed report of BBTV in South Africa (Jooste et al., 2016). In Australia, BBTV is restricted to northern New South Wales and southern Queensland. Quarantine measures are in force to prevent its introduction to northern Queensland and other states (Thomas and Iskra-Caruana, 1999).

It is virtually impossible to state for many countries whether the virus is native or exotic. There is little doubt that BBTV is now native in many countries, but there is also some evidence that it was inadvertently disseminated to many of them in infected vegetative propagules (probably during the twentieth century) (A Brunt, personal communication, 2003).

Distribution Table

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The distribution in this summary table is based on all the information available. When several references are cited, they may give conflicting information on the status. Further details may be available for individual references in the Distribution Table Details section which can be selected by going to Generate Report.

Continent/Country/RegionDistributionLast ReportedOriginFirst ReportedInvasiveReferenceNotes

Asia

BangladeshPresentCABI/EPPO, 2013; EPPO, 2014
CambodiaAbsent, unreliable recordEPPO, 2014
ChinaRestricted distribution Invasive CABI/EPPO, 2013; EPPO, 2014
-FujianPresent Invasive Zhou and Xie, 1992; Zhou and Zheng, 2001; CABI/EPPO, 2013; EPPO, 2014
-GuangdongPresent Invasive Zhou and Xie, 1992; He et al., 2001; CABI/EPPO, 2013; EPPO, 2014
-GuangxiPresent Invasive Zhou and Xie, 1992; CABI/EPPO, 2013; EPPO, 2014
-HainanPresentFeng et al., 2010; CABI/EPPO, 2013
-Hong KongAbsent, unreliable recordEPPO, 2014
-YunnanPresent Invasive Zhou and Xie, 1992; CABI/EPPO, 2013; EPPO, 2014
IndiaRestricted distribution Invasive CABI/EPPO, 2013; EPPO, 2014
-Andhra PradeshPresent Invasive CABI/EPPO, 2013; EPPO, 2014
-AssamPresent Invasive CABI/EPPO, 2013; EPPO, 2014
-KarnatakaPresent Invasive Sharma, 1988; CABI/EPPO, 2013; EPPO, 2014
-KeralaPresent Invasive CABI/EPPO, 2013; EPPO, 2014
-MaharashtraPresent Invasive CABI/EPPO, 2013; EPPO, 2014
-MeghalayaPresentCABI/EPPO, 2013
-OdishaPresent Invasive CABI/EPPO, 2013; EPPO, 2014
-Tamil NaduPresent Invasive Thiribhuvanamala & Doraiswamy, 2001; Govindaswamy et al., 1977; CABI/EPPO, 2013; EPPO, 2014
-Uttar PradeshPresent Invasive Khurana, 1971; CABI/EPPO, 2013; EPPO, 2014
-West BengalPresentCABI/EPPO, 2013
IndonesiaPresent Invasive CABI/EPPO, 2013; EPPO, 2014
-Irian JayaAbsent, formerly presentEPPO, 2014
-JavaPresent Invasive Sulyo and Muharam, 1985; CABI/EPPO, 2013; EPPO, 2014
-KalimantanPresent Invasive Sulyo and Muharam, 1985; CABI/EPPO, 2013; EPPO, 2014
-Nusa TenggaraPresentCABI/EPPO, 2013; EPPO, 2014
IranRestricted distributionBananej et al., 2007; CABI/EPPO, 2013
JapanRestricted distribution Invasive CABI/EPPO, 2013; EPPO, 2014
-Bonin IslandPresent Invasive Gadd, 1926
-Ryukyu ArchipelagoRestricted distribution Invasive Kawano and Su, 1993; CABI/EPPO, 2013; EPPO, 2014
Korea, Republic ofAbsent, reported but not confirmedCABI/EPPO, 2013; EPPO, 2014
LaosAbsent, unreliable recordEPPO, 2014
MalaysiaRestricted distribution Invasive CABI/EPPO, 2013; EPPO, 2014
-Peninsular MalaysiaAbsent, formerly presentCABI/EPPO, 2013; EPPO, 2014
-SabahAbsent, unreliable recordEPPO, 2014
-SarawakPresent Invasive CABI/EPPO, 2013; EPPO, 2014
MyanmarPresentCABI/EPPO, 2013; EPPO, 2014
NepalAbsent, invalid recordIPPC, 2005; CABI/EPPO, 2013
PakistanRestricted distributionIntroduced Invasive Khalid et al., 1993; CABI/EPPO, 2013; EPPO, 2014
PhilippinesRestricted distribution Invasive Castillo and Martinez, 1961; CABI/EPPO, 2013; EPPO, 2014
Sri LankaPresent Invasive Bryce, 1920; CABI/EPPO, 2013; EPPO, 2014
TaiwanPresent Invasive Sun, 1961; CABI/EPPO, 2013; EPPO, 2014
ThailandPresentCABI/EPPO, 2013; EPPO, 2014
VietnamPresent Invasive Vakili, 1969; CABI/EPPO, 2013; EPPO, 2014

Africa

AngolaPresentKumar et al., 2009; CABI/EPPO, 2013; EPPO, 2014
BeninPresentLokossou et al., 2012; CABI/EPPO, 2013; EPPO, 2014
BurundiPresent Invasive Thomas et al., 1994; CABI/EPPO, 2013; EPPO, 2014
CameroonRestricted distributionIPPC, 2010; CABI/EPPO, 2013; EPPO, 2014
Central African RepublicPresent Invasive Thomas et al., 1994; Diekmann and Putter, 1996; CABI/EPPO, 2013; EPPO, 2014
CongoPresent Invasive Thomas et al., 1994; CABI/EPPO, 2013; EPPO, 2014
Congo Democratic RepublicPresent Invasive CABI/EPPO, 2013; EPPO, 2014
EgyptPresent Invasive Magee, 1927; CABI/EPPO, 2013; EPPO, 2014
Equatorial GuineaPresentCABI/EPPO, 2013; EPPO, 2014
EritreaPresentCABI/EPPO, 2013; EPPO, 2014
GabonWidespread Invasive Fouré and Manser, 1982; CABI/EPPO, 2013; EPPO, 2014
MalawiWidespread Invasive Kenyon et al., 1997; CABI/EPPO, 2013; EPPO, 2014
MozambiqueRestricted distributionGondwe et al., 2007; CABI/EPPO, 2013; EPPO, 2014; IPPC, 2017
NigeriaRestricted distributionAdegbola et al., 2013; CABI/EPPO, 2013; EPPO, 2014
RwandaPresent Invasive Thomas et al., 1994; CABI/EPPO, 2013; EPPO, 2014
South AfricaPresent2015CABI/EPPO, 2013; EPPO, 2014; Jooste et al., 2016
ZambiaPresentGondwe et al., 2007; CABI/EPPO, 2013; EPPO, 2014

North America

USARestricted distribution Invasive CABI/EPPO, 2013; EPPO, 2014
-HawaiiPresent Invasive Dietzen & Thomas, 1991; Hu et al., 1993; CABI/EPPO, 2013; EPPO, 2014

South America

BrazilAbsent, intercepted onlyIntroduced Not invasive Oliveira et al., 2002

Oceania

American SamoaPresent Invasive CABI/EPPO, 2013; EPPO, 2014
AustraliaRestricted distribution Invasive CABI/EPPO, 2013; EPPO, 2014
-New South WalesRestricted distribution Invasive Magee, 1927; CABI/EPPO, 2013; EPPO, 2014
-QueenslandRestricted distribution Invasive CABI/EPPO, 2013; EPPO, 2014
FijiPresent Invasive Pearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014
French PolynesiaAbsent, invalid recordEPPO, 2014
GuamPresent Invasive Pearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014
KiribatiAbsent, invalid recordPearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014
Micronesia, Federated states ofAbsent, unreliable recordEPPO, 2014
New CaledoniaRestricted distributionIntroduced Invasive Kagy et al., 2001; Pearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014
Northern Mariana IslandsAbsent, unreliable recordCABI/EPPO, 2013; EPPO, 2014
PalauAbsent, invalid recordCABI/EPPO, 2013; EPPO, 2014
Papua New GuineaAbsent, unreliable recordEPPO, 2014
SamoaPresent Invasive Pearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014
TongaPresent Invasive Pearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014
TuvaluPresent Invasive Pearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014
Wallis and Futuna IslandsPresent Invasive Pearson and Grisoni, 2002; CABI/EPPO, 2013; EPPO, 2014

Risk of Introduction

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Great care should be taken to prevent the dissemination of BBTV to areas where it does not occur. The Americas are free of the disease, but the aphid vector, Pentalonia nigronervosa, is present and the disease would be expected to cause very serious problems if it appeared in smallholdings in the Caribbean and Central or South America. No movement should occur other than by tissue culture. However, the transmission of BBTV in micropropagated cultures has been demonstrated (Drew et al., 1989).

Infected plantlets were indistinguishable from uninfected control plantlets in culture (Drew et al., 1992). When plantlets were deflasked after 12 months in culture (as proliferating tissue) and allowed to grow, all plants developed severe symptoms within 1 month and all had died by 4 months. After 16 months in culture, 75% of plantlets showed symptoms, although it took up to 6 months for all plants to develop symptoms. The other 25% grew normally and appeared healthy (Drew et al., 1992). They were later tested for BBTV with negative results.

Recent findings indicate that proliferating tissue cultures initiated from meristems from BBTV-infected plants may eventually 'lose' the virus after many years under slow growth conditions (DR Jones, Montpellier, personal communication, 1995), but it would be advisable to test all germplasm from BBTV-affected areas before meristems are taken to initiate cultures. BBTV has been eliminated from Lakatan (AAA) by heat-treating shoot-tip cultures for extended periods (Ramos and Zamora, 1990). Subsequently, it was reported that the uneven distribution and low concentration of BBTV after exposure of proliferating tissue cultures to heat leads to BBTV-free primordial cells, which in turn develop into healthy plants (Wu and Su, 1991).

 

Hosts/Species Affected

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In the Musaceae, BBTV is known to infect a range of Musa species, cultivars in the Eumusa (derived mainly from M. acuminata and M. acuminata x M. balbisiana) and Australimusa (derived mainly from M. maclayi, M. lolodensis and M. peekelii) series of edible banana and Ensete ventricosum (enset). Susceptible Musa species include M. balbisiana (Magee, 1948; Espino et al., 1993), M. acuminata ssp. banksii, M. textilis (abacá) (Magee, 1927), M. velutina (Thomas and Dietzgen, 1991), M. uranoscopos, M. jackeyi, M. ornata and M. acuminata ssp. zebrina (ADW Geering and JE Thomas, Brisbane, personal communication, 1998).

To date, there are no confirmed reports of immunity to BBTV in any Musa species or cultivar. However, differences in susceptibility between cultivars subject to either experimental or field infection have frequently been noted (Magee, 1948; Muharam, 1984; Espino et al., 1993).

Espino et al. (1993) evaluated a total of 57 banana cultivars for their reaction to bunchy top, both by experimental inoculation and field observations. All cultivars in the AA and AAA genomic groups were highly susceptible. However, low levels of infection (as assessed by symptom expression) or total absence of symptoms following aphid inoculation was noted in some cultivars containing the B genome. These included 'Radja' (AAB, syn. 'Pisang Raja' - 12.5% of inoculated plants with symptoms), 'Bungaoisan' (AAB, Plantain subgroup - 0%), 'Pelipia' (ABB, syn. Pelipita' - 10%), 'Pundol' (ABB - 0%), 'Katali' (ABB, syn. 'Pisang Awak' - 0%), 'Abuhon' (ABB - 0%) and 'Turangkog' (ABB - 0%).

These cultivars were not back-indexed by aphid transmission to a susceptible banana cultivar or tested biochemically (for example, by ELISA), so the presence of symptomless infection cannot be ruled out. Also, greater numbers of aphids than the 15 used here may have resulted in infection. Cultivars 'Abuhon' and 'Bungaoisan' are susceptible to BBTV by experimental aphid inoculation (ADW Geering and JE Thomas, Brisbane, personal communication, 1998). Nevertheless, it appears that real differences exist in cultivar reaction to bunchy top and the time taken before symptoms are expressed.

Cultivars within the Cavendish subgroup form the basis of the international banana export trade and are generally highly susceptible to bunchy top. However, it appears that not all cultivars with an AAA genome are similarly susceptible. 'Gros Michel' exhibits resistance to the disease under both experimental inoculation and field conditions and Magee (1948) considered that the introduction of this cultivar to Fiji in the early 1900s contributed to partial rehabilitation of the bunchy top-devastated industry. Compared to 'Williams' (AAA, Cavendish subgroup), the concentration of virions of BBTV in infected plants of 'Gros Michel' and the proportion of plants infected by aphid inoculation is lower. Symptoms are also slower to develop and are less severe (ADW Geering and JE Thomas, Brisbane, personal communication, 1997). These factors may contribute to a reduced rate of aphid transmission and field spread in plantations of 'Gros Michel'.

There is some evidence for hosts outside the Musaceae, though reports have been conflicting. Su et al. (1993) obtained positive ELISA reactions from BBTV-inoculated Canna indica and Hedychium coronarium, and recovery of the virus to banana, though not reported here, was demonstrated (HJ Su, Taipei, personal communication, 1996). Ram and Summanwar (1984) reported Colocasia esculenta as a host of BBTV. However, Hu et al. (1996) were unable to demonstrate C. esculenta or Alpinia purpurata as experimental (E) or natural (N) hosts of BBTV in Hawaii. Geering and Thomas (1997) also found no evidence for the following species as hosts of BBTV in Australia: Strelitzia sp. (N), C. indica (E, N), C. x generalis (N), C. x orchiodes (N), H. coronarium (E), Helocania psittacorum (E), Alpinia coerulea (E, N), A. arundinelliana (E), A. zerumbet (E), Alocasia brisbaensis (E, N) or C. esculenta (E, N). Magee (1927) was unable to infect Strelitzia sp., Ravenala sp., Canna sp. (including C. edulis), Solanum tuberosum and Zea mays.

Primary hosts are banana cultivars derived from M. acuminata and M. acuminata x M. balbisiana, and Musa textilis (abacá).

Host Plants and Other Plants Affected

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Plant nameFamilyContext
Musa (banana)MusaceaeMain
Musa acuminata (wild banana)MusaceaeMain
Musa textilis (manila hemp)MusaceaeMain
Musa x paradisiaca (plantain)MusaceaeMain

Growth Stages

Top of page Flowering stage, Fruiting stage, Vegetative growing stage

Symptoms

Top of page The typical symptoms of bunchy top of banana are very distinctive and readily distinguished from those caused by other viruses of banana. Plants can become infected at any stage of growth and there are some initial differences between the symptoms produced in aphid-infected plants and those grown from infected planting material.

In aphid-inoculated plants, symptoms usually appear in the second leaf to emerge after inoculation and consist of a few dark-green streaks or dots on the minor veins on the lower portion of the lamina. The streaks form 'hooks' as they enter the midrib and are best seen from the underside of the leaf in transmitted light. The 'dot-dash' symptoms can sometimes also be seen on the petiole. The following leaf may display whitish streaks along the secondary veins when it is still rolled. These streaks become dark green as the leaf unfurls. Successive leaves become smaller, both in length and in width of the lamina, and often have chlorotic, upturned margins. The leaves become dry and brittle and stand more erect than normal giving the plant a rosetted and 'bunchy top' appearance.

Suckers from an infected stool can show severe symptoms in the first leaf to emerge. The leaves are rosetted and small with very chlorotic margins that tend to turn necrotic. Dark-green streaks are usually evident in the leaves.

Infected plants rarely produce a fruit bunch after infection and do not fruit in subsequent years. Plants infected late in the growing cycle may fruit once, but the bunch stalk and the fruit will be small and distorted. On plants infected very late, the only symptoms present may be a few dark green streaks on the tips of the flower bracts (Thomas et al., 1994).

Mild strains of BBTV, which induce only limited vein clearing and dark-green flecks, and symptomless strains have been reported in Cavendish plants from Taiwan (Su et al., 1993). Mild disease symptoms are expressed in some banana cultivars and Musa species. The dark-green leaf and petiole streaks, so diagnostic and characteristic of infection of cultivars in the Cavendish subgroup, can be rare or absent (Magee, 1953). Some plants of 'Veimama' (AAA, Cavendish subgroup), after initial severe symptoms, have been observed to recover and to display few if any symptoms.

The symptoms of abacá bunchy top include a reduction in leaf size and lamina area, rosetting of leaves, upcurling and yellowing of leaf margins and stunting of the pseudostem. Chlorotic areas on the leaves sometimes collapse, becoming necrotic, and 'heart rot' often occurs in the pseudostem. No fruit is produced and infected plants usually die within 1 or 2 years (Magee, 1953; Wardlaw, 1961). Dark-green 'dots and dashes' on the minor veins, midribs and petioles occurs in only a few abacá cultivars. Magee (1953) concluded that in abacá 'the green streak symptom occurs so rarely as to lose nearly all its value as an aid in diagnosis'. Symptoms of abacá bunchy top in the field are very similar to those that develop on abacá inoculated with the bunchy top virus from banana. When enset is experimentally infected with BBTV, symptoms are similar to those described for abacá (Magee, 1953).

List of Symptoms/Signs

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SignLife StagesType
Fruit / abnormal shape
Inflorescence / lesions; flecking; streaks (not Poaceae)
Leaves / abnormal colours
Leaves / abnormal forms
Whole plant / distortion; rosetting
Whole plant / dwarfing

Biology and Ecology

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BBTV is transmitted by an aphid vector (Pentalonia nigronervosa) and is disseminated in vegetative planting material, but is not transmitted by mechanical inoculation (Magee, 1927).

Distribution and Movement within the Plant

Magee (1927) showed that banana bunchy top was systemic. Following aphid inoculation, symptoms generally do not appear until a further two or more leaves have been produced (Magee, 1927). This period can vary between 19 days in summer to 125 days in winter (Allen, 1978a). The virus can only be recovered by aphids from the first symptom leaf or those formed subsequently (Magee, 1940). Suckers produced on an infected stool generally develop symptoms before reaching maturity (Magee, 1927).

Magee (1939) also concluded that the virus was restricted to the phloem tissue. Microscopic examination revealed hypertrophy and hyperplasia of the phloem tissue and a reduction in the development of the fibrous sclerenchyma sheaths surrounding the vascular bundles. The cells surrounding the phloem contained abnormally large numbers of chloroplasts giving rise to the macroscopic dark-green streak symptom.

Subsequent investigation using RNA probes and PCR (Hafner et al., 1995) has demonstrated that BBTV replicates for a short period at the site of aphid inoculation, then moves down the pseudostem to the basal meristem, then finally to the corm, roots and newly formed leaves. Trace levels of virus were eventually detected by PCR in leaves formed prior to inoculation, but replication was not demonstrated. This latter observation is consistent with the inability to transmit the virus by aphids from such leaves (Magee, 1940) and with the sequential development of single, new leaves from the basal meristem.

BBTV has been detected by ELISA and/or PCR in most parts of the plant, including leaf lamina and midrib, pseudostem, corm, meristematic tissues, roots, fruit stalk and fruit rind (Thomas, 1991; Wu and Su, 1992; Hafner et al., 1995; ADW Geering and JE Thomas, Brisbane, personal communication, 1996).

Vegetative Propagation

BBTV is efficiently disseminated in conventional planting material including corms, bits and suckers. All suckers from an infected stool will eventually become infected. Magee (1927) demonstrated 100% transmission of bunchy top through new 'eyes' (meristematic growing points) even in a plant that had only been expressing symptoms for 2-3 weeks.

BBTV is also transmitted in micropropagated banana plants (Drew et al., 1989; Ramos and Zamora, 1990; Wu and Su, 1991) though not always at rates of 100%. From time to time, apparently virus-free meristems producing apparently virus-free plants can arise from an infected clone (Thomas et al., 1995).

In Pakistan in the early 1990s, much of the available planting material of 'Basrai' (AAA, Cavendish subgroup) was infected with BBTV. Plantations established from infected suckers and corms were completely unproductive.

Epidemiology of Banana Bunchy Top

The epidemiology of banana bunchy top in Australia is simplified by the presence of a single susceptible host and a single vector species (P. nigronervosa). Dissemination over long distances is by infected planting material and it is by this means that new plantings in isolated areas usually become infected. Spread over short distances from these infection foci is by the banana aphid.

In studies of outbreaks of bunchy top in commercial banana plantations, Allen (1978b, 1987) showed that the average distance of secondary spread of the disease by aphids was only 15.5-17.2 m. Nearly two-thirds of new infections were within 20 m of the nearest source of infection and 99% were within 86 m. Allen and Barnier (1977) showed that if a new plantation was located adjacent to a diseased plantation, the chance of spread of bunchy top into the new plantation within the first 12 months was 88%. This chance was reduced to 27% if the plantations were separated by 50-1000 m, and to less than 5% if they were 1000 m apart. On average, the interval between infection of a plant and movement of aphids from this plant to initiate new infections elsewhere (the disease latent period) was equivalent to the time taken for 3.7 new leaves to emerge. The rate of leaf emergence varied seasonally with a maximum in summer (Allen, 1987). On the basis of these studies, a computer program has been developed which simulates epidemics of bunchy top (RN Allen, Brisbane, 1994, personal communication). This program, which is commercially available, allows epidemiological factors to be varied and their effect on the progess of the epidemic and disease control to be monitored.

In the Philippines, Opina and Milloren (1996) also demonstrated that most new infections were adjacent to or in close proximity to primary sources of infection.

Means of Movement and Dispersal

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Aphid Transmission

In Australia, the banana aphid (Pentalonia nigronervosa) was considered to have a role in the etiology of banana bunchy top (Darnell-Smith, 1924) and in 1925 was conclusively demonstrated to be the vector (Magee, 1927). Banana aphids have a worldwide distribution with a host range that includes Musa textilis and other species in the Musaceae. Species in several closely related plant families including the Araceae (Alocasia sp., Caladium spp., Dieffenbachia spp., Xanthosoma sp.), Cannaceae (Canna spp.), Heliconiaceae (Heliconia spp.), Strelitzeaceae (Strelitzia spp.) and Zingiberaceae (Alpinia spp., Costus sp., Hedychium spp.) are also colonized (Wardlaw, 1961; RN Allen, Brisbane, personal communication, 1996). However, a degree of host preference is displayed and some difficulty can be experienced transferring them between host species. On banana plants in New South Wales, aphids are found at the base of the pseudostem at soil level and for several centimetres below the soil surface, beneath the outer leaf sheaths and on newly emerging suckers. Aphid numbers decrease during periods of drought (Wardlaw, 1961).

Transmission of BBTV is of the circulative, non-propagative type. The transmission parameters reported from Hawaii (Hu et al., 1996) and Australia (Magee, 1927), respectively, are: minimum acquisition access period 4 h/17 h; minimum inoculation access period 15 min/30 min-2 h; retention of infectivity after removal from virus source 13 days/20 days; similar parameters have also been reported by Thiribhuvanamala et al. (2001) from India. No evidence was found for transmission of BBTV to the parthenogenetic offspring (Magee, 1940; Hu et al., 1996) or for multiplication of BBTV in the aphid vector (Hafner et al., 1995).

Transmission efficiency for individual aphids has been reported as ranging from 46 to 67% (Magee, 1927; Wu and Su., 1990a; Hu et al., 1996) and the virus is more efficiently acquired by nymphs than by adults (Magee, 1940).

Colonies of P. nigronervosa from Australia (where bunchy top occurs) and from Reunion Island (where bunchy top does not occur) both transmitted each of six isolates of BBTV with similar efficiency (ML Iskra-Caruana, Montpellier, personal communication, 1994).
 

Plant Trade

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Plant parts liable to carry the pest in trade/transportPest stagesBorne internallyBorne externallyVisibility of pest or symptoms
Bulbs/Tubers/Corms/Rhizomes Yes Pest or symptoms usually invisible
Flowers/Inflorescences/Cones/Calyx Yes Pest or symptoms usually invisible
Fruits (inc. pods) Yes Pest or symptoms usually invisible
Leaves Yes Pest or symptoms usually visible to the naked eye
Roots Yes Pest or symptoms usually invisible
Seedlings/Micropropagated plants Yes Pest or symptoms usually visible to the naked eye
Stems (above ground)/Shoots/Trunks/Branches Yes Pest or symptoms usually invisible
Plant parts not known to carry the pest in trade/transport
Bark
Growing medium accompanying plants
True seeds (inc. grain)
Wood

Wood Packaging

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Wood Packaging not known to carry the pest in trade/transport
Loose wood packing material
Non-wood
Processed or treated wood
Solid wood packing material with bark
Solid wood packing material without bark

Vectors and Intermediate Hosts

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VectorSourceReferenceGroupDistribution
Pentalonia nigronervosaInsect

Impact Summary

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CategoryImpact
Animal/plant collections None
Animal/plant products None
Biodiversity (generally) None
Crop production Negative
Environment (generally) None
Fisheries / aquaculture None
Forestry production None
Human health None
Livestock production None
Native fauna None
Native flora None
Rare/protected species None
Tourism None
Trade/international relations Negative
Transport/travel None

Impact

Top of page BBTV is the most serious virus disease of bananas and plantains. Devastating epidemics occurred in Fiji at the turn of the century and in Australia in the 1920s. The economic effect in northern New South Wales was dramatic. Between 1922 and 1926, about 90% of the area under bananas had gone out of production, despite a rapid expansion of the industry to compensate for abandoned farms. In the Currumbin district in southern Queensland, the number of plantations fell from 100 to 4 between 1922 and 1925, and production plummeted by over 95% (Dale, 1987). In recent years, bunchy top disease has been decimating the banana industry in Pakistan. In the early 1990s, statistics revealed that the area under banana cultivation in Sindh province fell by 55% in one year (Jones, 1994). BBTV is a major constraint to production in many areas where it occurs.

Diagnosis

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Prior to 1990, the only assays available for banana bunchy top were visual assessment of symptoms and aphid transmission to a sensitive banana cultivar. Subsequently, both serological and nucleic acid-based assays have become available.

Polyclonal and monoclonal antibodies are now routinely used in ELISA to detect BBTV in field and tissue culture plants and can detect the virus in single viruliferous aphids (Wu and Su, 1990b; Dietzgen and Thomas, 1991; Thomas and Dietzgen, 1991; Thomas et al., 1995). Triple antibody sandwich-ELISA was found to be the optimum assay format for routine virus indexing (Geering and Thomas, 1996). Double antibody sandwich (DAS)-ELISA has also been used to successfully detect BBTV (Prakash et al., 2010). All isolates tested from Africa, Australia, Asia and the Pacific region are serologically related (Thomas, 1991).

A variety of nucleic acid-based assays have been applied to the detection of BBTV in plant tissue and viruliferous aphids, including DNA and RNA probes, labelled either non-radioactively or with 32P (Hafner et al., 1995; Xie and Hu, 1995). PCR has proved to be about one thousand times more sensitive than ELISA or dot blots with DNA probes (Xie and Hu, 1995; Zhou and Zheng, 2001; Manickam et al., 2002). Substances in banana sap inhibitory to PCR can be circumvented by simple extraction procedures (Thomson and Dietzgen, 1995) or by immunocapture PCR (Anceau, 1996; M Sharman, JE Thomas and RG Dietzgen, Brisbane, personal communication, 1997).

Detection and Inspection

Top of page The presence of 'dot-dash' symptoms on leaves almost certainly indicates that a plant is infected with BBTV. The rosetted appearance of leaves which are progressively shorter, narrower and more upright is noticeable at a distance. Yellowing of the leaf margins is also indicative, though this can be caused by other problems.

Prevention and Control

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Regulatory Control

BBTV has not been eradicated from any country where it occurs, but it is believed to have been eliminated from certain banana-growing districts in Australia. Here, the disease is kept in check by strict State Government legislation which controls the source and movement of planting material, controls the issue of planting permits and requires the destruction of feral plants and all plants with symptoms. Banana inspectors are also employed to police these regulations and locate diseased and feral plants. A new, ambitious programme of eradication is being initiated which is based on replacing plantations where the disease regularly occurs, with BBTV-tested, tissue-cultured, planting material (Thomas et al., 1994).

Host-Plant Resistance

Little testing of germplasm for resistance has been undertaken, but it has been thought that all cultivars are susceptible, although some may take longer to develop symptoms and others may escape infection because of aphid preferences or host morphological factors. However, work in Australia suggests that Musa coccinea, a wild species, and the cultivar Kluai Teparot (ABBB/ABB) may have physiological resistance (J Thomas, QDPI, Indooroopilly, Queensland, Australia, personal communication, 1995).

Cultural Control

Banana bunchy top disease can be effectively controlled by the eradication of diseased plants and the use of virus-tested planting material. Before destruction, diseased plants should first be sprayed with power kerosene or insecticide to kill all viruliferous aphids. The whole stool, including corm and all associated suckers, must then be destroyed by uprooting and chopping into small pieces or by herbicide treatment, as the virus will ultimately spread to all parts of the mat. Control must be practised across the whole production area to avoid the rapid re-infection of virus-tested planting material (Thomas et al., 1994).

Chemical Control

Aphicides have been used in some countries to control populations of Pentalonia nigronervosa, the aphid vector of bunchy top, and a decrease in disease incidence has been reported. In Tonga, Aphidius colemani, a parasitic wasp, has been released in an attempt at biological control of the aphid vector, but its effects on disease incidence have been disappointing.

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