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Datasheet

Azolla filiculoides (water fern)

Summary

  • Last modified
  • 22 November 2017
  • Datasheet Type(s)
  • Invasive Species
  • Preferred Scientific Name
  • Azolla filiculoides
  • Preferred Common Name
  • water fern
  • Taxonomic Tree
  • Domain: Eukaryota
  •   Kingdom: Plantae
  •     Phylum: Pteridophyta
  •       Class: Filicopsida
  •         Family: Azollaceae
  • Summary of Invasiveness
  • A. filiculoides is a small fern native to the Americas which has spread widely throughout the world by a variety of mechanisms, of which man has become the most significant (...

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Pictures

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PictureTitleCaptionCopyright
Azolla filiculoides (water or fairy fern); sample on human index finger, showing scale. UK, 2008.
TitleSingle plant
CaptionAzolla filiculoides (water or fairy fern); sample on human index finger, showing scale. UK, 2008.
Copyright©CABI-2008
Azolla filiculoides (water or fairy fern); sample on human index finger, showing scale. UK, 2008.
Single plantAzolla filiculoides (water or fairy fern); sample on human index finger, showing scale. UK, 2008.©CABI-2008
Azolla filiculoides (water fern); habit, clogging water surface. UK, 2006.
TitleHabit
CaptionAzolla filiculoides (water fern); habit, clogging water surface. UK, 2006.
CopyrightCABI-2006/Richard Shaw
Azolla filiculoides (water fern); habit, clogging water surface. UK, 2006.
HabitAzolla filiculoides (water fern); habit, clogging water surface. UK, 2006.CABI-2006/Richard Shaw
Azolla filiculoides (water fern); habit, clogging water surface. Note Coot (Fulica atra) struggling to swim through the infestation. UK, 2003.
TitleHabit
CaptionAzolla filiculoides (water fern); habit, clogging water surface. Note Coot (Fulica atra) struggling to swim through the infestation. UK, 2003.
Copyright©CABI-2003/Richard Shaw
Azolla filiculoides (water fern); habit, clogging water surface. Note Coot (Fulica atra) struggling to swim through the infestation. UK, 2003.
HabitAzolla filiculoides (water fern); habit, clogging water surface. Note Coot (Fulica atra) struggling to swim through the infestation. UK, 2003.©CABI-2003/Richard Shaw
Azolla filiculoides (water fern); habit, clogging water surface. (note several Lemna minor plants also present)
TitleHabit
CaptionAzolla filiculoides (water fern); habit, clogging water surface. (note several Lemna minor plants also present)
Copyright©Mygaia at en.wikipedia (T.M.McKenzie) - CC BY-SA 3.0
Azolla filiculoides (water fern); habit, clogging water surface. (note several Lemna minor plants also present)
HabitAzolla filiculoides (water fern); habit, clogging water surface. (note several Lemna minor plants also present)©Mygaia at en.wikipedia (T.M.McKenzie) - CC BY-SA 3.0
Azolla filiculoides (water or fairy fern); plant showing the roots.
TitleSingle plant showing roots
CaptionAzolla filiculoides (water or fairy fern); plant showing the roots.
Copyright©Mygaia at en.wikipedia (T.M. McKenzie) - CC BY-SA 3.0
Azolla filiculoides (water or fairy fern); plant showing the roots.
Single plant showing rootsAzolla filiculoides (water or fairy fern); plant showing the roots.©Mygaia at en.wikipedia (T.M. McKenzie) - CC BY-SA 3.0
Stenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.
TitleBio-control agent
CaptionStenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.
Copyright©CABI/Richard Shaw
Stenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.
Bio-control agentStenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.©CABI/Richard Shaw
Stenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.
TitleBio-control agent
CaptionStenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.
Copyright©CABI-2008/Richard Shaw
Stenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.
Bio-control agentStenopelmus rufinasus, a weevil (Coleoptera, beetle) used as a bio-control for A. filiculoides.©CABI-2008/Richard Shaw

Identity

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Preferred Scientific Name

  • Azolla filiculoides Lamarck

Preferred Common Name

  • water fern

Other Scientific Names

  • Azolla bonariensis Bertoloni
  • Azolla japonica Franch. & Sav.
  • Azolla magellanica Willd.
  • Azolla squamosa Molina

International Common Names

  • English: fairy moss; mosquito fern; Pacific azolla; red water fern; water fern; water velvet
  • Spanish: helecho acuatico; hlechito del aqua; lenteja de agua
  • French: fougere d'eau

Local Common Names

  • China: xi lu-ping; xi man jiang hong
  • Germany: grosser algenfarn
  • Japan: akaukikusa
  • Netherlands: grote Kroosvaren
  • South Africa: rooivaring

EPPO code

  • AZOFI (Azolla filiculoides)
  • AZOJA (Azolla japonica)

Summary of Invasiveness

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A. filiculoides is a small fern native to the Americas which has spread widely throughout the world by a variety of mechanisms, of which man has become the most significant (Lumpkin and Plucknett, 1982). Man has introduced A. filiculoides into Europe, North and sub-Saharan Africa, China, Japan, New Zealand, Australia, the Caribbean and Hawaii. In eutrophic water systems, A. filiculoides grows rapidly, easily outcompeting indigenous vegetation. Decaying root and leaf matter below a mat of A. filiculoides, coupled with the lack of light penetration, creates an anaerobic environment which can reduce the quality of drinking water and make survival for other organisms in the water impossible.

Taxonomic Tree

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  • Domain: Eukaryota
  •     Kingdom: Plantae
  •         Phylum: Pteridophyta
  •             Class: Filicopsida
  •                 Family: Azollaceae
  •                     Genus: Azolla
  •                         Species: Azolla filiculoides

Notes on Taxonomy and Nomenclature

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The origin of the generic name Azolla is thought to be derived from a conjugation of two Greek words meaning 'to dry' and 'to kill', inferring that the fern is killed by drought (Moore, 1969; Lumpkin and Plucknett, 1980).

The genus Azolla has no generally accepted taxonomic framework, primarily as a result of the plant's diminutive structure, morphological and phenotypic plasticity (Stergianou and Fowler, 1990; Saunders and Fowler, 1992) resulting in numerous synonyms. Currently, the genus is divided into three sections (Saunders and Fowler, 1992; Saunders and Fowler, 1993): section Azolla, which includes A. filiculoides Lam., A. rubra R. Br. (now more commonly regarded as a subspecies of A. filiculoides), A. caroliniana Willd., A. microphylla auct. non Kaulf, A. mexicana Presl and A. cristata Kaulf.; section Rhizosperma, which includes A. pinnata R. Br. var. africana (Desv.) R.M.K. Saunders and K. Fowler, stat. et comb. nov., A. pinnata R. Br. var. asiatica R.M.K. Saunders and K. Fowler, subsp. nov., and A. pinnata R. Br. var. pinnata; and section Tetrasporocarpia, which includes A. nilotica Decne. ex Mett. Taxonomic problems have primarily centred around the closely related species in section Azolla (Nayak and Singh, 1989; Stergianou and Fowler, 1990). A study of specimens by Evrard and Van Hove (2004) concluded that only two species exist in America and, according to the priority rule for nomenclature, they should be named A. filiculoides and A. cristata

Description

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A. filiculoides is a small aquatic heterosporous fern, rarely larger than 25 mm (O'Keeffe, 1986). The genus is unique in that it grows in association with a heterocystous cyanobacterium (blue-green alga), Anabaena azollae Strasburger (Nostocales: Nostocaceae), which is located in cavities in the dorsal leaf-lobes (Ashton and Walmsley, 1984). This symbiotic association is the only one known between a pteridophyte and a cyanobacterium (Ashton and Walmsley, 1984). The Azolla macrophyte consists of a main rhizome, which branches into secondary rhizomes. These all bear alternately arranged small leaves. Ventrally, unbranched adventitious roots hang down into the water from nodes. Nutrients are absorbed directly from the water by the roots. In very shallow water, however, the roots may touch the soil thus deriving nutrients from it (Wagner, 1997). Rao (1936) reported that in A. pinnata, once the roots attain a length of 40-50 mm they drop off. Root hairs found along the length of the root provide accommodation for a large number of protozoa, algae and soil particles (Rao, 1936).

Plant Type

Top of page Aquatic
Perennial
Seed propagated
Vegetatively propagated

Distribution

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According to Lumpkin and Plucknett (1980), A. filiculoides is native to the Rocky Mountain states of the western USA and Canada, through Central America and to most of South America. It has been introduced to Europe, North and sub-Saharan Africa, China, Japan, New Zealand, Australia, the Caribbean and Hawaii.

Distribution Table

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The distribution in this summary table is based on all the information available. When several references are cited, they may give conflicting information on the status. Further details may be available for individual references in the Distribution Table Details section which can be selected by going to Generate Report.

Continent/Country/RegionDistributionLast ReportedOriginFirst ReportedInvasiveReferenceNotes

Asia

ChinaPresentIntroducedReed, 1954
IndiaPresentParimal and Kushari, 2002
IranPresentIntroduced Invasive Khoshravesh et al., 2009
IsraelPresent, few occurrencesEPPO, 2014
JapanPresentIntroducedSeto and Nasu, 1975
TurkeyPresentEPPO, 2014

Africa

EgyptPresentMagda et al., 2002; EPPO, 2014
MozambiquePresentIntroduced Invasive Langa, 2013
South AfricaWidespreadIntroduced1948 Invasive Twyman and Ashton, 1972

North America

Canada
-British ColumbiaPresentNativeUSDA-ARS, 2003
MexicoPresentNativeSvenson, 1944
USAPresentEPPO, 2014
-AlabamaPresentNativeRicherson and Grigarick, 1967
-AlaskaPresentNativeSvenson, 1944
-ArizonaPresentNativeRicherson and Grigarick, 1967
-CaliforniaPresentNativeRicherson and Grigarick, 1967
-FloridaPresentNativeMadeira et al., 2013
-HawaiiPresentIntroducedFosberg, 1942
-IllinoisPresentNativeSvenson, 1944
-LouisianaPresentNativeRicherson and Grigarick, 1967
-MississippiPresentNativeRicherson and Grigarick, 1967
-NevadaPresentNativeUSDA-NRCS, 2002
-OregonPresentNativeUSDA-NRCS, 2002
-TexasPresentNative Invasive Richerson and Grigarick, 1967
-UtahPresentNativeSvenson, 1944
-WashingtonPresentNativeUSDA-NRCS, 2002
-WisconsinPresentNativeSvenson, 1944

Central America and Caribbean

Costa RicaPresentNativeUSDA-ARS, 2003
GuatemalaPresentNativeUSDA-ARS, 2003
HondurasPresentNativeUSDA-ARS, 2003
NicaraguaPresentNativeUSDA-ARS, 2003
Trinidad and TobagoPresentIntroducedReed, 1965

South America

ArgentinaPresentNativeReed, 1962
BoliviaPresentNativeSvenson, 1944
BrazilPresentNativeUSDA-ARS, 2003
-AmazonasPresentNativeReed, 1962
-ParaPresentNativeReed, 1962
-Rio Grande do SulPresentNativeReed, 1962
-Sao PauloPresentNativeTofoli et al., 2000
ChilePresentNativeReed, 1962
ColombiaPresentNativeUSDA-ARS, 2003
EcuadorPresentNativeUSDA-ARS, 2003
French GuianaPresentNativeSvenson, 1944
ParaguayPresentNativeUSDA-ARS, 2003
PeruPresentNativeReed, 1962
UruguayPresentNativeReed, 1962
VenezuelaPresentNativeUSDA-ARS, 2003

Europe

BelgiumPresentIntroducedLawalree, 1964; EPPO, 2014
BulgariaPresentIntroducedSourek, 1958; EPPO, 2014
CroatiaPresentIntroducedEPPO, 2014
Czech RepublicPresentIntroducedEPPO, 2014
Czechoslovakia (former)PresentIntroducedSourek, 1958
FrancePresentIntroduced Invasive Chevalier, 1926; EPPO, 2014
GermanyPresentIntroduced Invasive Schloemer, 1953; Hussner et al., 2010; EPPO, 2014
GreecePresentIntroducedRoyal Botanic Garden Edinburgh, 2003; EPPO, 2014
HungaryPresentIntroduced Invasive Royal Botanic Garden Edinburgh, 2003; EPPO, 2014
IrelandPresentIntroduced Invasive Brunker, 1949; EPPO, 2014
ItalyPresentIntroduced Invasive Royal Botanic Garden Edinburgh, 2003; EPPO, 2014
-SardiniaPresentIntroducedEPPO, 2014
NetherlandsPresentIntroduced Invasive Sculthorpe, 1967; Pratt et al., 2013; EPPO, 2014
PolandPresent, few occurrencesIntroduced Invasive EPPO, 2014
PortugalPresentIntroduced Invasive Reed, 1962; EPPO, 2014
RomaniaPresentIntroducedLawalree, 1964; EPPO, 2014
Russian FederationPresentIntroducedEPPO, 2014
-Central RussiaPresentIntroducedEPPO, 2014
SpainPresentNative Invasive Royal Botanic Garden Edinburgh, 2003; EPPO, 2014
SwedenPresentEPPO, 2014
UKPresentIntroduced1921 Invasive Royal Botanic Garden Edinburgh, 2003; EPPO, 2014
UkrainePresentEPPO, 2014

Oceania

AustraliaPresentIntroducedBailey, 1902
-VictoriaPresentIntroducedAllinson et al., 2000
New ZealandPresentIntroducedBailey, 1902; Large and Braggins, 1993

History of Introduction and Spread

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A. filiculoides, the type species of the genus, is widely distributed, having been introduced to a number of countries in which it is not indigenous (Ashton, 1982). The plant has been dispersed by a variety of mechanisms, of which man has become the most significant (Lumpkin and Plucknett, 1982).
 
According to Szczesniak et al. (2009), A. filiculoides was first recorded in Europe towards the end of the nineteenth century, and the first observations were made in 1870s-1880s. The species may have been accidentally transported in ballast tanks of ships, in water with fry, or directly as an ornamental. Janes (1998a) noted the deliberate introduction of the plant as an ornamental into Europe through mainland Britain at the end of the nineteenth century. Possibly as a result of its various transport routes, A. filiculoides appeared independently in different places at almost the same time. It then spread across nearly all of Europe. 
 
A. filiculoides was introduced into Asia from East Germany in 1977 as an alternative to the cold susceptible native strain of A. pinnata, used as a green manure in the rice industry (Lumpkin and Plucknett, 1982). It was introduced to Africa in 1948 as an aquarium plant (Oosthuizen and Walters, 1961; Jacot-Guillarmod, 1979).
 
A. filiculoides has also been spread around the world as a model plant for the study of Azolla-Anabaena symbiosis (Carrapiço, 2010),

Introductions

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Introduced toIntroduced fromYearReasonIntroduced byEstablished in wild throughReferencesNotes
Natural reproductionContinuous restocking
China Germany 1977 Horticulture (pathway cause) Yes Lumpkin and Plucknett, 1980 Introduced for green fertiliser
Egypt 1980 Horticulture (pathway cause) Yes Searg et al., 2000
Germany 1899 Yes Hussner et al., 2010
India   Horticulture (pathway cause) Yes Lumpkin and Plucknett, 1980 Introduced for green fertiliser
Iran   Horticulture (pathway cause) Yes Sadeghi et al., 2012 Introduced for green fertiliser
Italy   Horticulture (pathway cause) Yes Introduced for green fertiliser
Mozambique South Africa 2000 Yes Langa, 2013 Natural dispersal
Poland early 1900s Aquaculture (pathway cause) Yes Szczesniak et al., 2009
Portugal 1930 Horticulture (pathway cause)Garcia-Murillo et al., 2007 Introduced for green fertiliser
South Africa Brazil 1948 Horticulture (pathway cause) Yes Hill, 1999
Spain 1957 Horticulture (pathway cause) Yes Garcia-Murillo et al., 2007
UK 1920 Horticulture (pathway cause) Yes Janson, 1921
Zimbabwe South Africa 1995 Yes McConnachie et al., 2003 Natural dispersal

Risk of Introduction

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Further spread is likely as A. filiculoides continues to be sold in nurseries as a fish pond plant. In Africa and Europe, dispersal between countries will no doubt continue due to the movement of waterfowl, which can spread plant fragments between bodies of water. A. filiculoides will continue to invade countries where the presence of eutrophic waters, lack of natural enemies and an unregulated nursery trade will contribute to its status as a weed.

Habitat

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A. filiculoides in its native areas (South America and western North America) is a plant of slow flowing streams and rivers, ponds and lakes (Reed, 1962; Lumpkin and Plucknett, 1980; Ashton, 1982). Its native range is characterised by warm, tropical climates with humid summers and mild winters. It has commonly been utilised as an ornamental in fishponds and tanks and has spread from these foci, exhibiting a weedy phenology in nutrient enriched reservoirs and roadside canals (T. Center, Senior Researcher, Aquatic Weeds, United States Department of Agriculture, personal communication).

Habitat List

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CategoryHabitatPresenceStatus
Freshwater
Freshwater Present, no further details Harmful (pest or invasive)
Terrestrial-natural/semi-natural
Wetlands Present, no further details Harmful (pest or invasive)

Hosts/Species Affected

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A. filiculoides is not generally considered a weed of crops. It is commonly grown in conjunction with rice, as A. filiculoides has the ability to fix nitrogen via an endosymbiotic blue-green alga (Wagner, 1997), acting as a green manure in rice cultivation. However, in its introduced range, A. filiculoides may impact upon trout and other fish farming (McConnachie et al., 2003). It has also been recorded to affect the growth of Potamogeton crispus L. (Janes et al., 1996).

Host Plants and Other Plants Affected

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Plant nameFamilyContext
Potamogeton crispus (curlyleaf pondweed)PotamogetonaceaeOther

Growth Stages

Top of page Vegetative growing stage

Biology and Ecology

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Genetics

The chromosome number of A. filiculoides is 2n=44 (Stergianou and Fowler, 1990). This number is shared by A. pinnata, A. caroliniana, A. microphylla and A. mexicana. Hybridization has been recorded with A. microphylla under laboratory conditions (van Cat et al., 1989), the artificial hybrid having the same chromosome number as its parents (Stergianou and Fowler, 1990). Hybrids were found to produce only microsporocarps, had intermediate stem lengths, and grew better than A. microphylla in the field (van Cat et al., 1989).

Physiology and Phenology

Megaspores are produced in spring and summer, and can overwinter and survive extreme desiccation (Lumpkin and Plucknett, 1982). Megaspores germinate on the mud surface at the bottom of a waterbody, often in very shallow (0.1-0.2 m) areas. Ashton (1982) conducted detailed studies regarding A. filiculoides spore germination. The first visible sign of germination is the upward displacement of the indusium cap, due to the expansion of the first leaf. Eventually, as the second and third leaves start pushing upwards, the indusium cap is lost and the new sporophyte floats to the water surface where further development takes place. The first root appears from the base of the first leaf, and at this point the remains of the old megaspore break away from the sporophyte. Continued development results in the rapid elongation of the rhizome and the production of further leaves and roots.

Reproductive Biology

A. filiculoides is able to undergo rapid vegetative reproduction throughout the year by the elongation and fragmentation of the small fronds, and under ideal conditions, the daily rate of increase can exceed 15% with the doubling time being every 4-5 days (Lumpkin and Plucknett, 1982). Under favourable environmental conditions, A. filiculoides undergoes sexual reproduction. Pairs of sporocarps are formed from a ventral lobe initial of a lateral branch. The sporocarps are of two types, male microsporocarps and female megasporocarps (Ashton, 1982; Wagner, 1997). There is usually a pair of either microsporocarps or megasporocarps, but one of each may be present (Moore, 1969). Microsporocarps are approximately 1.5 mm in diameter, containing 8-130 microsporangia (Wagner, 1997). Within each microsporangium there are 64 microspores, which are, in turn, aggregated into 3-10 massulae (Moore, 1969). Megasporocarps are approximately 0.5 mm in diameter, each producing a single megasporangium (Wagner, 1997). A single megaspore, which contains a small colony of Anabaena azollae is contained within the megasporangium (Wagner, 1997). Upon reaching maturity, both micro- and megasporocarps dehisce. Microsporangia release spongy masses of massulae into the water, which attach to megasporocarps via barbed, protruding appendages (glochidia) (Lumpkin and Plucknett, 1980). These entanglements usually sink to the bottom of a waterbody and, after a period of dormancy, the micro- and megaspores will germinate to form prothalli (Wagner, 1997). Ciliated, male gametes (antherozoids) develop in antheridia on the male thallus and female gametes (oospheres) develop in archegonia on the female thallus. After fertilization of the oospheres by the antherozoids, an embryo develops (Moore, 1969). Ashton (1982) found megaspore germination to be affected by desiccation (desiccation for >40 days greatly reduced germination); turbulence (severe turbulence lowered germination levels to a low 6%); photoperiod; light intensity; and pH (no germination occurred at pH levels <4.5 or >9.5).

Associations

A. filiculoides grows in association with the heterocystous cyanobacterium (blue-green alga) Anabaena azollae (Nostocales: Nostocaceae), within the dorsal leaf lobe cavities (Ashton and Walmsley, 1984). The alga has the ability to fix atmospheric nitrogen and is able to fulfil the nitrogen requirements of the fern making it successful in nitrogen-deficient waters (Ashton, 1982).

Environmental Requirements

A. filiculoides is an obligate aquatic plant, whose growth is phosphorous limited. Climatic requirements include suitably warm months for sporocarp development, adequate radiation and light intensity for vegetative growth, and adequate amounts of rainfall to prevent its aquatic habitat from drying up. This species of tropical origin is thought to have evolved a cold-tolerant strain since its introduction into Britain (Janes, 1998b) and South Africa (McConnachie, 2003). A. filiculoides may be able to survive temperatures as low as -10ºC before death occurs.
 

Latitude/Altitude Ranges

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Latitude North (°N)Latitude South (°S)Altitude Lower (m)Altitude Upper (m)
40 30 0 4362

Air Temperature

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Parameter Lower limit Upper limit
Absolute minimum temperature (ºC) -10
Mean annual temperature (ºC) 20 28
Mean maximum temperature of hottest month (ºC) 18 39
Mean minimum temperature of coldest month (ºC) -4 15

Rainfall

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ParameterLower limitUpper limitDescription
Dry season duration00number of consecutive months with <40 mm rainfall
Mean annual rainfall40545mm; lower/upper limits

Rainfall Regime

Top of page Summer
Uniform

Natural enemies

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Natural enemyTypeLife stagesSpecificityReferencesBiological control inBiological control on
Pseudolampsis darwinii Herbivore Leaves to genus Hill, 1999
Pseudolampsis guttata Herbivore Leaves Hill and Oberholzer, 2002
Stenopelmus brunneus Herbivore Leaves to species
Stenopelmus rufinasus Herbivore Leaves to genus Hill, 1998 South Africa

Notes on Natural Enemies

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Host records from around the globe show that the genus Azolla is attacked by generalist herbivores and that very few specialist insect species have evolved on these plants (Hill, 1997). However, four beetle species, the weevils Stenopelmus rufinasus and S. brunneus and the two flea beetles Pseudolampsis guttata and P. darwinii, appear to have specialized on the genus Azolla (Richerson and Grigarick, 1967; Habeck, 1979; Hill, 1999) and were identified as potential biological control agents for A. filiculoides in South Africa (Hill, 1997). Following host range testing, Stenopelmus rufinasus was released in 1997 as a biocontrol of A. filiculoides in South Africa (McConnachie et al., 2004).

Means of Movement and Dispersal

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Natural Dispersal

A. filiculoides propagates both asexually and sexually. Both spores and plant fragments are dispersed long distances along river systems.

Vector Transmission

Waterfowl, amphibians and rodents are thought to spread small fragments of the plant that adhere to their bodies.

Agricultural Practices

A. filiculoides has been introduced in tropical regions worldwide as a green manure in rice cultivation.

Intentional Introduction

A. filiculoides was intentionally introduced into South Africa as a fish pond plant in 1948 (Jacot-Guillarmod, 1979) and by the early 1980s it was thought to have invaded every river system in South Africa. The plant was also deliberately introduced into south-east UK at the end of the nineteenth century as an ornamental plant (Janes, 1998a) and is now naturalised in numerous still and slow-flowing waters (Preston and Croft, 1997). There are also instances of research collections that have escaped from culture; A. filiculoides has been spread around the world as a model plant to study the Azolla-Anabaena symbiosis (Carrapiço, 2010)

Pathway Vectors

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VectorNotesLong DistanceLocalReferences
Soil, sand and gravelRivers Yes

Plant Trade

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Plant parts liable to carry the pest in trade/transportPest stagesBorne internallyBorne externallyVisibility of pest or symptoms
Growing medium accompanying plants seeds Yes
Roots seeds Yes
Plant parts not known to carry the pest in trade/transport
Bark
Bulbs/Tubers/Corms/Rhizomes
Flowers/Inflorescences/Cones/Calyx
Fruits (inc. pods)
Leaves
Seedlings/Micropropagated plants
Stems (above ground)/Shoots/Trunks/Branches
True seeds (inc. grain)
Wood

Impact Summary

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CategoryImpact
Animal/plant collections Negative
Animal/plant products Negative
Biodiversity (generally) Negative
Crop production Positive
Environment (generally) Negative
Fisheries / aquaculture Negative
Forestry production None
Human health Negative
Livestock production Negative
Native fauna Negative
Native flora Negative
Rare/protected species Negative
Tourism Negative
Trade/international relations Negative
Transport/travel Negative

Economic Impact

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The economic impact of A. filiculoides in South Africa was examined by McConnachie et al. (2003). Thick mats on reservoirs and slow-moving waterbodies caused economic losses to water-users. Among those water-uses most seriously affected were farming (71%), recreational (24%), and municipal (5%). On average, A. filiculoides was found to cause on-site damages of US$589 per hectare per year.

Environmental Impact

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In eutrophic water systems, A. filiculoides grows rapidly, easily outcompeting indigenous vegetation. Decaying root and leaf matter below a mat of A. filiculoides, and the lack of light penetration, creates an anaerobic environment. Not only can very little survive under such conditions, but the quality of drinking water is reduced, caused by bad odours, colour and turbidity (Hill, 1997). Cases have been reported where both livestock and game farmers have lost animals due to them refusing to drink from infested waterbodies or drowning as a result of mistaking the mat for solid ground. The weed also reportedly increases water loss through evapotranspiration and promotes the development of waterborne, water-based and water-related diseases (Hill, 1997).

Impact on Biodiversity

A. filiculoides infestations may form thick mats (5-20 cm thick), on waterbodies up to 10 hectares in size (McConnachie et al., 2003). Such infestations have been shown to severely impact the biodiversity of aquatic ecosystems and have serious implications for all aspects of water utilisation (Gratwicke and Marshall, 2001).
One of the last remaining habitats of the endangered fish species, the eastern Cape rocky (Sandelia bainsii Castelnau, 1861; Anabantidae) in South Africa, had become so overgrown with the weed that had the biological control programme not been so successful, S. bainsii faced extinction.

Threatened Species

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Threatened SpeciesConservation StatusWhere ThreatenedMechanismReferencesNotes
Sandelia bainsiiEN (IUCN red list: Endangered) EN (IUCN red list: Endangered)South AfricaCompetition - shadingHill, 1999

Social Impact

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Primarily, social impacts of A. filiculoides have centred around the reduction of useful water surface area for recreation (fishing, swimming and water skiing) and water transport.

Risk and Impact Factors

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Uses

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Members of the genus Azolla are utilized throughout the world for a wide variety of purposes besides its widespread uses as an ornamental in fish ponds and tanks (Lumpkin and Plucknett, 1980; 1982). A. filiculoides is used as a green manure in rice paddies, mainly in Asia, as an inhibitor of weed growth in rice cultivation in China and Vietnam (Kröck and Alkämper, 1991), and as an alternative high protein fodder for cattle, swine, poultry and fish, and possibly as an alternative food source for humans, again, mainly in Asia. It has also been used as a nitrate-rich compost which potentially increases soil organic nitrogen levels and cation exchange capacity. It is used for purification of water, removal of heavy metals (Sanyahumbi et al., 1998) and removal of nitrogen and phoshorous from wastewater (Forni et al., 2001). It has also been used variously as an ingredient in soap production, a cure for sore throats and as a control for mosquitoes in southern India as complete mats disrupt larval development (Rajendran and Reuben, 1991).

Similarities to Other Species/Conditions

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A. filiculoides is difficult to distinguish from A. microphylla, A. mexicana, A. caroliniana and A. cristata due to the variable morphology of the plant modified by interacting environmental influences (Ashton, 1982). Cytological studies are the most reliable method of separating these species (Stergianou and Fowler, 1990; Saunders and Fowler, 1992; 1993). Under field conditions, however, the most reliable measure of separating these species would be by means of their sporocarp structure (Svenson, 1944; Sweet and Hills, 1971). A. filiculoides: glochidia not septate, or rarely with 1 or 2 septae at the apex, microsporangia 35-100 in an indusium, massulae 4-6, megasporangia with raised, irregular hexagonal markings; A. caroliniana: glochidia not septate, microsporangia 8-40 in an indusium; A. mexicana: glochidia multi-septate, microsporangia usually with 4 massulae, megaspore pitted; A. microphylla: glochidia multi-septate, megaspore smooth. For comparisons including A. cristata, see Evrard and Van Hove (2004).

Prevention and Control

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Cultural Control

Even though A. filiculoides has been used as fodder for pigs, poultry and cattle (Lumpkin and Plucknett, 1982), control through grazing may not be achieved because of its aquatic habit. However, A. filiculoides may actually be a preferred forage for herbivorous fish, even over most other aquatic weeds (Edwards, 1974).

Mechanical Control

Small infestations of the weed in accessible areas may be removed using rakes and fine meshed nets (Hill, 1997). The disadvantage of mechanical control, however, is that under ideal conditions, the weed can double itself every 4-5 days (Lumpkin and Plucknett, 1982). Hence a concerted effort would be required just to keep up with the daily production of even a small infestation, and even if eradication were achieved, re-establishment from spores resident in the waterbody substrate would be inevitable. Ashton (1992) noted that A. filiculoides was susceptible to fragmentation from physical disturbance, and that the fragments were highly sensitive to high light intensity and were killed by direct sunlight. He thus suggested the use of a mechanical agitator or stirrer to provide enough turbulence to break up the plants. The cost, however, of such an approach (even on a small scale) would be prohibitive.

Chemical Control

Chemicals proposed to control A. filiculoides include glyphosate (Steyn et al., 1979; Ashton, 1992), paraquat and diquat (Axelsen and Julien, 1988), and kerosene mixed with a surfactant (Diatloff and Lee, 1979). However, paraquat is now banned in the EU, Switzerland and a number of other countries. Diquat use in the EU is restricted to terrestrial treatments and can no longer be authorised for aquatic weed control. Glyphosate is toxic to fish and algae and the water cannot be used for irrigation or stock until the herbicide has broken down.

Biological Control

South Africa is the only country that has initiated a classical biological control programme against A. filiculoides. Four insect species were identified as potential biological candidates - all frond-feeding beetles: Pseudolampsis guttata Leconte (Chrysomelidae), P. darwinii Sherer, Stenopelmus rufinasus Gyllenhal (Curculionidae) and S. brunneus Hustache . All species do extensive damage to the plants in the country of origin (Hill, 1997). The biology and host range of P. guttata was investigated by Hill (2002), and although it was found to be fairly damaging, the weevil S. rufinasus was identified as the most suitable of the four for release in South Africa, and was imported into quarantine for host-specificity screening. The weevil was released in 1997 and results have been dramatic, causing local extinction of A. filiculoides at the sites where it was released (McConnachie et al., 2004). The surface area of weed controlled totalled 203.5 ha and infested sites were controlled in approximately seven months on average (in a range of 3-11 months). Five years after the release of the weevil, A. filiculoides no longer poses a threat to aquatic ecosystems in South Africa and its effects on the utilization of water resources have been significantly reduced (McConnachie et al., 2003). S. rufinasus has been released in Mozambique and Zimbabwe with material provided by South Africa, has established, and is proving a successful biocontrol agent in these countries also (Cilliers et al., 2003).

S. rufinasus has been present in the UK since it was first reported there by Janson (1921) and was probably brought into Europe with the plant. Since then it has been recorded in Ireland, France, Belgium, the Netherlands and Spain (Pratt et al., 2013). This agent has been used as in an augmentive manner in England and Ireland and is under consideration for mass-rearing and releasing in the Netherlands, France and Belgium (Pratt et al., 2013).

Integrated Control

Because of the success of the biological control of A. filiculoides, there has been no need to develop an integrated approach.

 

Case Study: A. filiculoides in South Africa

A. filiculoides was first recorded in South Africa in 1948 from the Oorlogspoort River in the Northern Cape Province of South Africa (30°37’58.22”S 25°21’28.89”E), and by 1999 it was recorded from 152 sites in South Africa, largely in the Free State Province.

Owing to the adverse environmental and economic effects of the weed, a biological control programme was initiated with the importation of the weevil Stenopelmus rufinasus Gyllenhal (Coleoptera: Curculionidae) from Florida, USA, in 1995. Following host-specificity testing, the weevils were released in South Africa in 1997. By 2004, nearly 25,000 weevils had been released throughout South Africa, and their feeding damage has resulted in local extinctions of A. filiculoides from the majority of sites that were surveyed at the time. It took, on average, ten months for a site to be cleared after release of the weevils. The dispersal abilities of the weevils were originally underestimated, but they are capable of dispersing up to 350 km unaided.

Just five years after the release of the weevils, A. filiculoides was no longer considered a threat to South African waterbodies (McConnachie et al., 2004). These successes were carefully monitored between 1999 and 2006, documenting the rapid control of the plant, and proving that just four years after the studies by McConnachie et al. (2004) the weevils had succeeded in controlling A. filiculoides at every site where they had been released (Hill et al., 2008).

Country-wide surveys from 2008 produced further evidence of the success of this program. In 2010, of the 102 A. filiculoides sites investigated (40% of water weed sites surveyed), the weed was present at 19 (19%) of these sites, and S. rufinasus was recorded from 14 (73%) of the infested sites. A. filiculoides is no longer a significant problem in South Africa, and where it does occur, S. rufinasus is usually present. Biological control of A. filiculoides is now widely regarded as the most successful biological control programme against an invasive alien weed in South Africa (Coetzee et al., 2011). 

Field surveys indicated that the agent had started to use another host thought to be the native A. pinnata subsp. africana, however a recent study by Madeira et al. (2016) showed that it was in fact feeding on A. cristata, another introduced fern that is more closely related to A. filiculoides.

Gaps in Knowledge/Research Needs

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Further research on the taxonomy of the genus Azolla is on-going (Madeira et al., 2013, 2016).  

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Contributors

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27/06/2014 Datasheet updated by:

Martin Hill, Rhodes University, South Africa

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