Seiridium cardinale
(cypress canker)

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Identity

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Preferred Scientific Name

• Seiridium cardinale (W.W. Wagener) B. Sutton & I.A.S. Gibson

Preferred Common Name

• cypress canker

Other Scientific Names

• Coryneum cardinale W.W. Wagener

International Common Names

• English: canker of cypress; cypress blight
• Spanish: cancro de la corteza del ciprés; cancro del ciprés
• French: chancre cortical du cyprès; chancre du cyprès
• Portuguese: cancro cortical dos ciprestes

Local Common Names

• Germany: Zypresse Krebs
• Italy: cancro corticale del cipresso; cancro del cipresso

EPPO code

• SEIRCA (Seiridium cardinale)

Summary of Invasiveness

Top of page The high pathogenicity of S. cardinale, wide host range in the Cupressaceae family, relative stability of its virulence, abundant production of asexual spores, its adaptation to various environments and the possibility of long-distance transport by vectors or trade of infected propagation material, have allowed this fungus to spread widely and to cause pandemics in several continents.

Taxonomic Tree

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• Domain: Eukaryota
•     Kingdom: Fungi
•         Phylum: Ascomycota
•             Subphylum: Pezizomycotina
•                 Class: Sordariomycetes
•                     Subclass: Sordariomycetidae
•                         Order: Xylariales
•                             Family: Amphisphaeriaceae
•                                 Genus: Seiridium
•                                     Species: Seiridium cardinale

Notes on Taxonomy and Nomenclature

Top of page The fungus responsible for a destructive outbreak of canker disease on Cupressus macrocarpa and C. sempervirens in California (USA) was first described by Wagener in 1939 as Coryneum cardinale. It was later reassigned to the anamorph genus Seiridium Sutton and Gibson. So far, the teleomorph (tentatively Ascomycetes: Amphisphaeriaceae) has not been found. No subspecific entity or forma specialis of the fungus has been reported (Wagener, 1939; Sutton and Gibson, 1972; Boesewinkel, 1983; Graniti, 1986).

Description

Top of page The subperidermal acervular conidiomata of C. cardinale appear as minute, black pustular bodies scattered or clustered on infected stems, branches and cones of affected trees, and dehisce by rupture of the upper wall. Often black conidial masses spread from cankers over the bark.

Conidia are formed at the apices of hyaline, holoblastic, annellidic conidiogenous cells and subsequent proliferations. Conidia are oblong-fusiform, smooth, 17-34 (mostly 21-26) x 7-12 (mostly 8-10) µm (length/width ratio: 2.5-3), straight, sometimes slightly curved, 5-distoseptate. The thick-walled four median cells are of the same brown or dark colour, slightly collapsed when conidia are not fully turgid. The two thin-celled end cells are hyaline, the apical cell is campanulate, and the basal one truncate. The apical end cell bears a very short (approximately 1 µm long) hyaline appendage; the basal end cell often bears a similar, central appendage.

A transient production of hyaline, filiform spermatia may occur within the acervuli (Motta, 1979).

For further details, see Wagener, 1939; Sutton and Gibson, 1972; Sutton, 1975; Boesewinkel, 1983; Graniti, 1986, 1998a; Nag Raj, 1994.

Distribution

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After the introduction of cypress canker in France and Italy around the middle of the last century (Barthelet and Vinot, 1944; Grasso, 1951), the wide occurrence of susceptible hosts and climatic conditions favourable to growth and dissemination of the pathogen, facilitated its establishment and spread in the Mediterranean area (Solel et al., 1983; Xenopoulos and Diamandis, 1985; Graniti, 1986, 1998a; Raddi et al., 1987; Luisi, 1990, Panconesi, 1990).

There is an old record for New South Wales, Australia (Hutton, 1949), however this is not supported by any more recent literature.

The list of countries includes records of specimens from the IMI Herbarium retained at CABI Bioscience, UK Centre, Egham; dates of collection are noted (Herb. IMI, various dates).

Distribution Table

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The distribution in this summary table is based on all the information available. When several references are cited, they may give conflicting information on the status. Further details may be available for individual references in the Distribution Table Details section which can be selected by going to Generate Report.

History of Introduction and Spread

Top of page An outbreak of destructive cypress blight was first reported from northern California, USA, in 1939, but the pathogen (S. cardinale) had probably been introduced into the area more than a decade earlier (Wagener, 1928, 1939, 1948, 1964). The main effect of this epidemic was on the highly susceptible Monterey cypress, Cupressus macrocarpa and, to a lesser extent, on the European C. sempervirens and on some species of American and exotic Cupressaceae. In the same years the disease was reported from New Zealand (Birch, 1933), and subsequently spread into Europe (Barthelet and Vinot, 1944), Asia Minor, South America, Australia, Japan and South Africa. To date, the disease has become established in the boreal latitudes between 30° and 40°N and in some austral areas between 30° and 50°S (CABI/EPPO, 2003)

The origin of S. cardinale in producing the first epidemics remains uncertain. A virulent strain able to infect susceptible cypress trees may have arisen from local populations of weakly pathogenic Seiridium species in the areas where the disease was first recorded (Wagener, 1964). The most likely hypothesis is that the first epidemics originated from accidental introduction of the pathogen into California, USA, or New Zealand on imported nursery stocks of ornamental cypress trees.

Risk of Introduction

Top of page S. cardinale is a highly dangerous pathogen, able to cause serious epidemics to susceptible Cupressaceae in temperate areas of the world. Although the most common vehicle for the worldwide diffusion of cypress canker has been through the international trade in infected nursery stock, S. cardinale has not been included in the EPPO lists of quarantine organisms (EPPO, 1987).

The risk of introduction of the pathogen into hitherto free areas is not limited to accidental transport or trade of infected seeds, seedlings, potted plants and nursery stock, but also includes the trade of infected corticated, and even decorticated, timber.

Habitat

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No natural or experimental host species of S. cardinale is known out of the family Cupressaceae. Canker disease may affect groups of trees in natural forests, woods, stands, plantations, and rows of windbreaks, as well as single ornamental cypress trees in parks, gardens, cemeteries and historical places. Nurseries and propagation plots are also affected.

Habitat List

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Hosts/Species Affected

Top of page S. cardinale may infect and cause disease to many species, varieties and hybrids of Cupressaceae, both native and cultivated. Susceptibility to S. cardinale canker disease varies among and within the host species.

Inoculation tests on a number of known and potential hosts in various countries and environments indicated that Cupressus macrocarpa is highly susceptible, C. sempervirens, Thuja plicata and Cupressocyparis leylandii are less susceptible; Cupressus arizonica, C. lusitanica, C. forbesii, Chamaecyparis lawsoniana, T. orientalis [Platycladus orientalis] and other species show a range of resistance, whereas Cupressus bakeri, C. torulosa, C. funebris [Chamaecyparis funebris], C. cashmeriana and other Asiatic species of Cupressus were resistant or highly resistant (Smith, 1938; Wagener, 1939, 1948; Wolf, 1939; Wolf and Wagener, 1948; Grasso, 1952; Strouts, 1973; Faddoul, 1973; Raddi and Panconesi, 1977; Ponchet and Andréoli, 1979; Grasso et al., 1979; Grasso and Ponchet, 1980; Andréoli, 1979; Beresford and Mulholland, 1982; Mathon, 1982; van der Werff, 1988; Valdivieso et al., 1988; Panconesi, 1990; Andréoli and Ponchet, 1991; Xenopoulos, 1991a, 1991b; Spanos, 1995; Spanos et al., 1997a; Teissier du Cros, 1999; Ducrey et al., 1999).

Relative susceptibility of potential hosts to the pathogen has been tested in several countries under natural, greenhouse and laboratory conditions. Degree of susceptibility or resistance of the host to a particular strain of S. cardinale, a mixture of strains, or samples representing large populations of the pathogen, is usually assessed by inoculating cypress seedlings in the greenhouse or young trees in the nursery or field. Such tests, however, may take 1-2 years and up to 8 years for reliable results to be obtained, depending on genotype and age of the tree, and on environmental conditions (Panconesi, 1990).

Susceptibility of cypress clones or virulence of S. cardinale strains can be comparatively assessed by the size of the necrotic lesion at cambium level, as measured on decorticated stems of inoculated seedlings (Ponchet and Andréoli, 1984).

Correlations between responses of in vitro or in greenhouse inoculated seedlings, micropropagated shoots, explants or tissue cultures and known levels of field resistance have been reported. Their application in resistance breeding programmes could be advantageous in terms of accuracy and speed, especially if associated with responses to toxin treatment.

Early screening methods to assay cypress species or varieties, clones or progenies for resistance to S. cardinale, or low sensitivity to their toxins, have been set up. For example, either cypress explanta or callus cultures could be used to screen cypress genotypes prior to field evaluation. Direct inoculation of S. cardinale on callus cultures, inhibition of fungal growth by cypress callus in dual cultures, ion leakage or ethylene evolution from explanta treated with S. cardinale toxins may provide information about the susceptibility of clones to the pathogen or sensitivity to its toxic metabolites (Tonon, 1994; Tonon et al., 1995; Spanos and Woodward, 1997). The response of callus or cell cultures to seiridins could be used to screen cypress germplasm in vitro (Sparapano et al., 1986; Sparapano and Evidente, 1995).

Expression of resistance responses to S. cardinale in micropropagated cypress shoots has been evaluated histologically. Accumulation of oxidized phenolics or deposition of suberin and lignin in cell walls of foliar epidermis and hypodermis could be used to detect genotypes resistant to the pathogen (Spanos et al., 1997a).

Host Plants and Other Plants Affected

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Growth Stages

Top of page Flowering stage, Fruiting stage, Post-harvest, Vegetative growing stage

Symptoms

Top of page Cankers on stem and branches

Infection by S. cardinale on susceptible host trees induces both local and systemic symptoms.

The first sign of cypress blight by S. cardinale is a browning or a reddening of the live bark of stem or branches, at the point of entry of the pathogen. Discoloration is followed by a slight depression of the infected area, longitudinal cracking or fissuring, and resinous exudation. Subsequently, lenticular or elongated cankers develop on the bark around the infection site, where a necrosis of the infected bark tissue occurs, and these may girdle the branches or the stem of young plants. Outgrowths of bark tissues, histological abnormalities and plant cell necrosis may occur around the diseased areas. On trunk and large branches of adult trees, the enlargement of cankers is a slow process. Consistent flows of resin exuding from cracks formed on the cankered area can be seen on the outside of the cankers, which may extend to infected stems and branches. Usually, sectors of the tree on the side of the cankers decline and die.

Cuttings made through the inner bark, the cambium and the first few rings of sapwood can reveal a brown or reddish discoloration. Intensity of discoloration is variable and may assume a typical red-violet colour (hence, the specific epithet 'cardinale' given to the pathogen).

Foliage chlorosis and dieback of branches and top of trees

On trees of susceptible species, e.g. Cupressus macrocarpa and C. sempervirens, crown symptoms are clearly associated with presence of cankers. A diffuse yellowing or reddening first appears on the foliage of twigs, branches, and apical parts of the affected trees, subsequently turning to brown or reddish-brown as the dieback progresses. The leaves of affected branches become dry with time, and then slowly drop to the ground. Fading, drying and dieback of branches and treetops are the most conspicuous symptoms of the disease. The spread of one, several, or many infections on a single tree can kill the whole tree within a relatively short time, depending on its age, susceptibility and the environment.

On relatively resistant clones of Cupressus sempervirens, on C. arizonica, and even on more resistant species such as C. torulosa and C. lusitanica, infection can develop slowly. With resistant hosts, eventually the cankers can be compartmentalized and sealed off by the plant defence reactions (Ponchet and Andréoli, 1990; Ponchet et al., 1990).

List of Symptoms/Signs

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Biology and Ecology

Top of page Genetics

Although a teleomorph is unknown for S. cardinale, heterokaryosis is a common feature of this mitosporic fungus, and no evidence of vegetative incompatibility was found among strains (Sánchez and Gibbs, 1995); consequently, the occurrence of natural variants of the pathogen cannot be ruled out. However, current data do not support a condition of high variability in S. cardinale. Several works indicated that there is little variation in virulence for this pathogen (Andréoli et al., 1984; Panconesi, 1990; Xenopoulos, 1991b; Spanos, 1995).

The disease caused by S. cardinale on its hosts is markedly influenced by local ecological conditions. Canker disease is more common and severe in non-optimal climatic environments for growth of cypress, e.g. in the northwestern areas of the Mediterranean region (Quezal, 1985) than in the southeastern areas, where C. sempervirens is native (Santini et al., 1997a; Santini and Di Lonardo, 2000).

Physiology

Leaf symptoms on trees affected by S. cardinale may develop on the branches of affected trees regardless of the girdling effect of the cankers, for example on foliage distal from where the fungus can be isolated. In the infected bark, necrosis and disruption of host cells, outgrowths of tissues and other histological abnormalities may occur in advance of hyphal growth (Mutto and Panconesi, 1987; Ponchet and Andréoli, 1989, 1990).

This suggests that some extracellular metabolites produced by the fungus, other than those involved in breaking down the apoplastic structures of the host (cell wall-degrading enzymes such as polygalatturanases, xylanases, cellulases, Magro et al., 1982), such as toxins, play a role in pathogenesis.

Toxin Production

The nature and appearance of symptoms caused by infection of S. cardinale to its hosts, as well as the necrotic processes affecting bark and leaf tissues, suggest that toxins are produced in the cypress bark or wood colonized by the pathogen, and are possibly involved in pathogenesis. These substances may diffuse to adjacent tissues, and eventually translocate to distal parts and leaves via the transpiration stream. Out of 100 isolates of S. cardinale collected in Italy, 97 were toxigenic (Sparapano et al., 1995b).

Several phytotoxic metabolites produced by S. cardinale in culture were isolated and chemically characterized. The major toxins were two D<(sup)a,b> butenolides (seiridin and iso-seiridin), followed by three cyclic sesquiterpenes (seiricardines A, B and C) and two minor seiridins (Sparapano et al., 1986; Graniti and Sparapano, 1990; Ballio et al., 1991; Evidente and Sparapano, 1994; Graniti, 1998a).

At low concentrations (50 mM) seiridins enhance plant cell growth and can replace kinetin in tissue culture media. Assayed at higher concentrations (150 mM), seiridins induce leaf chlorosis and necrosis. A subperidermal injection of 2 ml of a 0.2-0.3 mg/ml solution of seiridin into the stem of susceptible cypress seedlings caused extensive dieback and death of the seedlings within 6-8 months (Sparapano et al., 1995b; Sparapano and Evidente, 1995). These symptoms were reminiscent of those shown by Seiridium-infected seedlings. Seiridins also showed antibacterial activity. The susceptibility of species of Cupressus to S. cardinale correlated with their sensitivity to seiridins. Inoculations with highly toxigenic isolates of S. cardinale killed only 5% of C. arizonica seedlings within 4 months compared to 30% of C. sempervirens and 75% of C. macrocarpa seedlings (Sparapano et al., 1994b).

Although minor metabolites, seiricardines as components of an array of toxins, may contribute to the overall toxicity of the pathogen. Injection of 3 ml of a 0.1 mg/ml solution of seiricardines A and B into the stem of young cypress trees induced hypertrophic reactions of bark tissue, longitudinal lesions on stems, and a reddish discoloration of distant leaves. All seiricardines showed fungistatic activity in vitro (Ballio et al., 1991; Evidente et al., 1993).

Reproductive Biology

Under favourable environmental conditions, production of conidia from acervular conidiomata formed on the surface of cankers on stem and branches, as well as on other parts of the affected tree such as the cones (galbuli), is able to assure the availability of fresh inoculum throughout the year. Moreover, conidia of S. cardinale retain their germinability and pathogenicity for more than 1 year (Wagener, 1939, 1948; Panconesi and Ongaro, 1982; Panconesi and Raddi, 1991b; Panconesi et al., 1993).

Pathogenesis

Penetration of S. cardinale hyphae through natural openings, i.e. stomata of leaves (or shoots) into the substomatal chamber, followed by invasion and deterioration of the mesophyll tissue, and lenticels, has been demonstrated experimentally. Penetration through the cuticle of leaves or young shoots is also possible; however, S. cardinale is unable to penetrate the periderm and lignified structures directly (Intini and Panconesi, 1976; Ponchet and Andréoli, 1989; Spanos et al., 1997a, 1999).

In the field and in the nursery, infection of S. cardinale on its hosts usually occurs through wounds produced by various agents: strong winds, frost, hail, insects, small animals and pruning. On young trees, infections are frequent at the insertion of shoots on the stem or branches, where wounds often occur and conidia carried by rain are laid. Actually, cypress canker has caused serious losses to cypress plantations and windbreaks in some Greek islands where winds are strong and recurrent (Xenopolous, 1991a) or in southern France and central Italy, where late frosts frequently occur (Dugelay, 1957; Moriondo, 1967).

After penetration, the pathogen grows within the bark, where a necrotic lesion develops. Spread of the mycelium is relatively rapid in the cortical parenchyma and less in the secondary phloem. Subsequently, the pathogen extends through the vascular cambium into the medullary rays and outermost layers of sapwood. Eventually, all bark tissues turn brown and die (Moriondo, 1972; Mutto and Panconesi, 1987; Ponchet and Andréoli, 1984, 1989; Madar and Liphschitz, 1989; Spanos et al., 1999). Cell necrosis of the cankered bark progresses steadily, with some seasonal variation, until the branches or stem are girdled. Relatively abundant flows of resin are produced by actively growing cankers, i.e. until they are able to enlarge. Even when fungal inoculum is placed deep into the stem, it can spread to the bark and give rise to cankers (Panconesi et al., 1995). The older bark is more resistant than younger bark to growth of the pathogen (Spanos et al., 1997a). Disorganization of xylem elements and occlusion of pit vessels with consequent reduction of water flow were observed in the xylem from branches of cypress trees inoculated with S. cardinale (Madar et al., 1990).

Host reaction to infection includes a range of cellular responses leading to cell wall incrustation by suberin and lignin, accumulation of oxidized polyphenolic compounds, and resinosis. Lignification, suberization and cell wall thickening have been observed in response to challenge by S. cardinale (Spanos and Woodward, 1997; Spanos et al., 1997a). Healing processes taking place in the surrounding tissues contribute to the formation of cankers. The different consistency of necrotic and reacting tissues and the consequent tension originate cracks and fissures on the bark through which resin can exude.

Resinosis as well as occlusions of the xylem elements most likely play a role in the plant's defence against a toxigenic pathogen that is invading the bark with potential systemic activity (Graniti, 1994; Sparapano et al., 1995b). The composition of foliage resin has been found to be different in healthy and S. cardinale-infected trees (Schiller and Madar, 1991).

Defence processes in infected trees take the form of separation of healthy bark tissue from the diseased one by the formation of a wound periderm through neophellogenic activity. The total thickness of this new periderm (more than 100 mm) was related to clonal resistance to S. cardinale (Ponchet and Andréoli, 1989), whereas in susceptible clones, a thinner periderm may be overcome by the pathogen, leading to the formation of a diffuse canker. In experiments with resistant cypress clones, development of boundary zones including 4-6 layers of cells with ligno-suberized walls within the diseased cypress bark tended to restrict and to isolate the tissues invaded by the pathogen, thus preventing its further spread. In susceptible clones, only 2-4 layers of suberized cells were formed in discontinuous bands around infection sites. These reactions involve a series of processes that can be detected histologically, allowing differentiation of resistant or tolerant species or clones from susceptible ones (Ponchet and Andréoli, 1990; Spanos et al., 1999).

The outer layers of the sapwood adjacent to cankers, as well as the medullar rays, may be colonized by S. cardinale, which can survive for a long period in the woody tissues of cypress without loss of pathogenicity (Faddoul, 1973; Mutto and Panconesi, 1987; Ponchet and Andréoli, 1989, 1990; Madar et al., 1990; Panconesi et al., 1995a).

Environmental Requirements

Conidia of S. cardinale can germinate, and mycelium grow in vitro, from 5-6°C up to a maximum of approximately 35°C, with optimal values around 25°C. Under natural environmental conditions, however, the disease develops with temperatures up to 30°C, although infection is optimal around 25°C (Graniti and Frisullo, 1990). Actually, growth of the pathogen in host tissues is slow or is even arrested during the hottest months of the year (Panconesi, 1990; Ponchet et al., 1990).

Relative humidity close to saturation is required for infection (at 80% RH about half of the conidia of the pathogen are unable to germinate). Rains are effective in spreading the inoculum and favouring penetration of the infecting hyphae through wounds. As a consequence, the incidence and severity of cypress blight may be high or even very high in areas where climatic factors, particularly rain and high relative humidity during the infection season (autumn through spring), favour the production and dissemination of inoculum, and where frost or strong winds produce wounds and lesions on cypress trees. Some cypress plantations close to a devastated area of high incidence may escape the disease for lack of just one predisposing factor, e.g. strong winds or high humidity.

Associations

S. cardinale is often associated with other dematiaceous fungi on cankered tissue of cypress bark, e.g. Pestalotiopsis funerea and P. monochaetioides; these are not primary pathogens however (Sánchez and Gibbs, 1995).

Natural enemies

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Notes on Natural Enemies

Top of page No natural enemies are known for S. cardinale, except competitors (such as species of Pestalotiopsis colonizing bark cankers) or antagonistic fungi (Trichoderma viride).

Means of Movement and Dispersal

Top of page Natural Dispersal

Under moist conditions, conidiomata of S. cardinale open wide on the surface of the cankers, thus exposing black slimy conidial masses. When dry, fragments of this material can be released into the environment by strong winds. Usually, however, conidia extruded from conidiomata are dispersed by rain over short distances, mostly in a downward direction, and then spread laterally by windborne conidia-laden droplets (Panconesi and Ongaro, 1982).

Vector Transmission

Long-distance spread of inoculum, even to isolated areas, is assured by insects and probably birds, which can carry inocula up to the tops of the tree. Insects, especially cork-borers, are highly efficient vectors. Twig-mining beetles such as Phloeosinus aubei, P. thujae and P. armatus are common in the Mediterranean area, and they can spread the disease either by carrying the inoculum from cankered trees into young shoots of healthy trees, or by opening wounds in the cypress bark through which rain-carried conidia enter and initiate infection (Wagener, 1939; Covassi et al., 1975; Mendel et al., 1983; Mendel, 1984; Sumer, 1987; Covassi, 1991; Tiberi and Battisti, 1998). On cones and seeds, the seed bug Orsillus maculatus and the seed chalcid Megastigmus wachtli may contribute to spread the disease (Tiberi and Battisti, 1998; Battisti and Roques, 1999; Roques and Battisti, 1999). Another insect vector of S. cardinale present in California, USA, is the cypress bark moth Cydia cupressana [Enarmonia cupressana] (Frankie and Koelher, 1971; Frankie and Parameter, 1972).

Seedborne Spread

The disease can be transmitted by contaminated or infected seed, both from cankered or healthy-looking trees.

Agricultural Practices

Infections by S. cardinale are favoured by any agricultural practice producing wounds.

Movement in Trade

The worldwide distribution of the disease has probably been favoured by the international trade of infected nursery stock, especially of ornamental species of Cupressaceae.

Seedborne Aspects

Top of page Incidence

Conidiomata of S. cardinale are frequently produced on cypress galbuli (Grasso, 1969). Seeds may also carry conidia on their surface or become infected by the pathogen with final formation of conidiomata (Motta and Saponaro, 1983; Saponaro and Motta, 1981, 1984; Motta, 1986).

Spraying a conidial suspension on recently fertilized ovules of C. sempervirens, C. macrocarpa and Thuja orientalis [Platycladus orientalis] resulted in a high disease incidence on mature seeds, and their germinability was sharply reduced (Motta, 1984).

Effect on Seed Quality

Cones infected by S. cardinale produce fewer filled seeds and more empty seeds than healthy cones. Seed damage by insects, such as the seed bug Orsellus maculatus and the seed chalcid Megastigmus wachtli, facilitates infection by S. cardinale (Tiberi and Battisti, 1998; Battisti et al., 2000). Seeds attacked by S. cardinale loose their germinability.

Pathogen Transmission

In a survey carried out with seeds from nine species of Cupressaceae from Italy and France, 0.5% to 70% of seeds were either surface-contaminated or infected by the pathogen even when collected from healthy-looking trees (Saponaro and Motta, 1984).

Seed Health Tests

The blotter method is commonly used for cypress seed testing (Saponaro and Motta, 1984). X-ray analysis has been used to assess cypress seed quality and the damage caused by insects and pathogenic fungi, including S. cardinale (Battisti et al., 2000).

Seed Treatments

Slurry dressing or immersion of seeds in an aqueous suspension of benomyl or thiophanate-methyl reduced infection from 47% (untreated) to 0-1% (Motta, 1984).

Plant Trade

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Wood Packaging

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Impact Summary

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Impact

Top of page Infection of susceptible cypress trees by S. cardinale under favourable environmental conditions is fatal. Death of the tree may take up to a few months or even years, depending on the species, clone, age, and environmental factors. In the Mediterranean region, where climatic conditions are favourable to the pathogen, the epidemic of cypress blight is so advanced that it has threatened to become an ecological disaster (Graniti, 1993, 1998a). Actually, about 85% of the existing population of C. sempervirens in Italy, estimated on seedlings from commercial seed, was found susceptible to the disease (Raddi and Panconesi, 1981b). In a similar survey carried out in Greece (Xenopoulos, 1990) with seedlings from natural stands, the percentages of susceptible cypress trees ranged from 97.2 (Crete) and 92.7 (Rhodes) to 88.6 (Samos).

Heavy economic losses have been caused by cypress canker to the ornamental trees industry, especially in districts like Provence in France and Tuscany in Italy, where nurseries are an important economic activity and cypresses represent a major part of the marketable production.

Moreover, cypress trees grow easily in poor, arid soils, and thus are almost irreplaceable in replanting degraded hilly areas and in reforestation. Several species of cypress are widely used as efficient windbreaks for citrus and other subtropical crops. Finally, cypress groves produce a highly valued timber.

Since its introduction, S. cardinale has caused destructive and recurrent epidemics that have devastated forests, natural stands, plantations, windbreaks and ornamental cypress trees in several countries of various parts of the world. The first epidemics in California, USA, resulted in a loss of about 30,000 trees of Cupressus macrocarpa and C. sempervirens (Wagener, 1939, 1948, 1964).

In central Italy (Tuscany), where the disease was first recorded more than 50 years ago, the most susceptible trees were killed in the first decades after introduction. The average incidence of canker on residual cypress plantations was estimated at 23.3% in 1995 (Pivi, 1995) and is currently 23-27%; but it has reached 75% in some groves around Florence (Panconesi, 1991; Panconesi and Raddi, 1998). A 1978 survey in the district of Florence showed that some 720,000 of the approximately 4 million cypress trees in the area (i.e., 18%) were either dead or severely affected by the disease (Poggesi, 1979). This figure would have been even higher (close to 1 million trees), if all diseased trees with only light infections (about 6%), which would die subsequently, had been considered. Further assessments were not made in the same area in the following years; meanwhile, the disease has progressed. A 7200-tree cypress grove near Florence was sampled every year to assess the disease incidence during the period 1981-1993. The relative figures were 31.3% (1981) and 50.6% (1993) incidence, representing a 19.3% increase in 12 years (Panconesi and Raddi, 1991b, 1998).

In Greece, the highest incidences were recorded in the areas around Kyrgia (70%), in the valley of Megalopolis, western Peloponnesus (90%), and around Karistos (98%), a windy valley of Euboea island, where cypresses are used extensively as windbreaks (Xenopoulos and Diamandis, 1985; Xenopoulos, 1991; Panconesi and Raddi, 1991). The annual increase of the disease in some stands in the Peloponnesus, with an initial attack of 20%, ranged from 5% to 20% (Xenopoulos, 1991a).

By contrast, the spread and severity of the cypress canker caused by S. cardinale have been low or virtually negligible in the warmest areas of the Mediterranean region, such as North Africa; however, in these areas, Lepteutypa cupressi represents a potential threat.

Economic Impact

Top of page Infection of susceptible cypress trees by S. cardinale under favourable environmental conditions is fatal. Death of the tree may take up to a few months or even years, depending on the species, clone, age, and environmental factors. In the Mediterranean region, where climatic conditions are favourable to the pathogen, the epidemic of cypress blight is so advanced that it has threatened to become an ecological disaster (Graniti, 1993, 1998a). Actually, about 85% of the existing population of C. sempervirens in Italy, estimated on seedlings from commercial seed, was found susceptible to the disease (Raddi and Panconesi, 1981b). In a similar survey carried out in Greece (Xenopoulos, 1990) with seedlings from natural stands, the percentages of susceptible cypress trees ranged from 97.2 (Crete) and 92.7 (Rhodes) to 88.6 (Samos).

Heavy economic losses have been caused by cypress canker to the ornamental trees industry, especially in districts like Provence in France and Tuscany in Italy, where nurseries are an important economic activity and cypresses represent a major part of the marketable production.

Moreover, cypress trees grow easily in poor, arid soils, and thus are almost irreplaceable in replanting degraded hilly areas and in reforestation. Several species of cypress are widely used as efficient windbreaks for citrus and other subtropical crops. Finally, cypress groves produce a highly valued timber.

Since its introduction, S. cardinale has caused destructive and recurrent epidemics that have devastated forests, natural stands, plantations, windbreaks and ornamental cypress trees in several countries of various parts of the world. The first epidemics in California, USA, resulted in a loss of about 30,000 trees of Cupressus macrocarpa and C. sempervirens (Wagener, 1939, 1948, 1964).

In central Italy (Tuscany), where the disease was first recorded more than 50 years ago, the most susceptible trees were killed in the first decades after introduction. The average incidence of canker on residual cypress plantations was estimated at 23.3% in 1995 (Pivi, 1995) and is currently 23-27%; but it has reached 75% in some groves around Florence (Panconesi, 1991; Panconesi and Raddi, 1998). A 1978 survey in the district of Florence showed that some 720,000 of the approximately 4 million cypress trees in the area (i.e., 18%) were either dead or severely affected by the disease (Poggesi, 1979). This figure would have been even higher (close to 1 million trees), if all diseased trees with only light infections (about 6%), which would die subsequently, had been considered. Further assessments were not made in the same area in the following years; meanwhile, the disease has progressed. A 7200-tree cypress grove near Florence was sampled every year to assess the disease incidence during the period 1981-1993. The relative figures were 31.3% (1981) and 50.6% (1993) incidence, representing a 19.3% increase in 12 years (Panconesi and Raddi, 1991b, 1998).

In Greece, the highest incidences were recorded in the areas around Kyrgia (70%), in the valley of Megalopolis, western Peloponnesus (90%), and around Karistos (98%), a windy valley of Euboea island, where cypresses are used extensively as windbreaks (Xenopoulos and Diamandis, 1985; Xenopoulos, 1991; Panconesi and Raddi, 1991). The annual increase of the disease in some stands in the Peloponnesus, with an initial attack of 20%, ranged from 5% to 20% (Xenopoulos, 1991a).

By contrast, the spread and severity of the cypress canker caused by S. cardinale have been low or virtually negligible in the warmest areas of the Mediterranean region, such as North Africa; however, in these areas, Lepteutypa cupressi represents a potential threat.

Environmental Impact

Top of page The devastation of planted and ornamental cypress trees has caused not only serious economic losses, but also other grim consequences for the environment and social life, including tourism. This holds true where cypress is not only a key component of the landscape and an irreplaceable decoration for monuments and historical places, like Florence in Italy and Olympia in Greece, but an integral part of the nation's history.

Diagnosis

Top of page A correct diagnosis of cypress canker and a proper identification of the pathogen are a prerequisite for determining actions to be taken. Identification of cypress blight does not present a problem for an experienced plant pathologist in areas where one or few cypress species or clones are grown, and where S. cardinale is the sole or the prevailing pathogenic species of Seiridium. When several species of Cupressaceae, often showing non-specific symptoms, are mixed in groves or parks, or when more than one pathogen is present, identification is more difficult and diagnosis is usually based both on microscopic examination of conidiomata or other reproductive structures formed in vivo, and by isolation and determination of the pathogen in culture. This is not always easy because the presence of the pathogen can be masked by other fungi (e.g. species of Pestalotiopsis) colonizing cankers.

Identification of S. cardinale is possible by microscopic examination of mitospores (conidia) produced on the surface of cankers or in culture. The six-celled conidia of S. cardinale are discernible from those of related species of Coelomycetes by the absence of long appendages at one or both conidial ends. Other methods, cultural, serological, chemical and molecular are available.

Detection and Inspection

Top of page The first symptom of the disease is noticeable from a distance as a change in colour of the foliage, which turns yellow and eventually brownish-red in dead trees. A diffuse foliar yellowing or reddening of twigs, branches and apical parts of the affected trees is a common characteristic of the disease. The flow of resin exuding from the cankers makes it easy to localize stem cankers hidden by the dense foliage of several cypress species. Such a resinous exudation, which is not present in cankers caused by other pathogens (e.g. Diaporthe occulta [D. eres]), may be abundant on the bark of the trunk or branches. Fading and dieback of twigs, branches and treetops are noticeable at distance, and these symptoms may facilitate a disease survey. Potential losses from disease in a cypress forest or plantation can be assessed from the air.

For disease assessment, either extension or size of cankers on stem or branches, and rating by visual scales of leaf damage and severity of dieback, are commonly used. As necrosis may extend from the cankered bark to the first layers of sapwood, the necrotic area can be seen (and measured) as a brown lesion of the wood surface in decorticated stems or trunks.

Similarities to Other Species/Conditions

Top of page Two more species with anamorphs in the genus Seiridium are known to cause canker diseases on various Cupressaceae: Lepteutypa cupressi (anamorph: Seiridium cupressi) and Seiridium unicorne (Swart, 1973; Sutton, 1975, 1980; Boesewinkel, 1983; Graniti, 1986; Graniti and Frisullo, 1990).

Lepteutypa cupressi caused serious losses to cypress plantations, especially to Cupressus macrocarpa, in Kenya in the 1940s (Nattrass and Ciccarone, 1947; Ciccarone, 1949; Nattrass et al., 1963), then in New Zealand (Boesewinkel, 1983) and in Australia. In the Mediterranean area, it was found in a natural forest of C. sempervirens on the Greek island of Kos (Graniti, 1986, 1998a; Xenopoulos, 1991a) and subsequently eradicated. Inoculation experiments with L. cupressi on susceptible hosts showed highest pathogenicity at 20-25°C. At 30°C the pathogen produces little disease (Graniti and Frisullo, 1990). After infection, L. cupressi progresses slowly during winter and faster during spring and early summer, with little or no growth at 35°C. On infected trees, the necrotic process continues even in the hottest months of the year, whereas that of S. cardinale is slowed. Hence, L. cupressi could potentially become established in the warmest Mediterranean areas (Panconesi, 1990).

Seiridium unicorne is a widespread and plurivorous fungus, which is common, but not serious, in several parts of the world (Portugal: Caetano et al., 1991; USA: Tisserat et al., 1991; New Zealand: van der Werff, 1988). Destructive epidemics of S. unicorne have not been reported. However, it has caused an epidemic of canker disease to the Hinoki cypress (Chamaecyparis obtusa) in Japan with serious losses to young plantations (Tabata, 1991).

S. cardinale can be distinguished from S. cupressi and S. unicorne on the basis of morphological and cultural traits, and this has been confirmed by physiological, pathogenic, toxicological and enzymatic polymorphism data (Boesewinkel, 1983; Graniti, 1986, 1998a; Graniti and Frisullo, 1990; Ponchet et al., 1990; Nag Raj, 1994; Raddi et al., 1994). In the past, some contrasting views were put forward, based in part on data from non-authenticated isolates, according to which, cypress canker is due to two species (with either presence or absence of long conidial appendages) or to only one species of variable morphology (Swart, 1973; Chou, 1989; Viljoen et al., 1993). More recently, distinction of three species of Seiridium affecting cypress trees was confirmed by phylogenetic and molecular data (PCR and SSCP or RFLP analysis of rDNA sequences: Moricca and Raddi, 1999, 2000; Moricca et al., 2001; histone and partial b-tubulin sequences: Barnes et al., 2001).

Fungi other than species of Seiridium are known to cause canker diseases on cypress in several countries. Among them, Sphaeropsis sapinea f.sp. cupressi (Solel et al., 1987; Frisullo and Graniti, 1990; Swart et al., 1993; Linde et al., 1997; Frisullo et al., 1997; Xenopoulos and Tsopelas, 2000); Botryosphaeria stevensii (Frisullo and Graniti, 1990); Diaporthe occulta [D. eres] (Farr et al., 1989); Lasiodiplodia theobromae (Bruck et al., 1990).

Dieback of shoots, a diffuse reddening and drying of foliage, and a general decline of trees can be caused by other adverse factors, e.g. frost damage and infestation by the aphid Cinara cupressi. The latter caused serious damage in central and southern Italy in the 1970s (Covassi and Binazzi, 1979; Binazzi et al., 1998).

Prevention and Control

Top of page Cultural Control and Sanitary Measures

Sanitation is the most efficient method to control epidemic spread of the disease. Localization of infected individual trees, their felling, and destruction of disease foci at their first appearance in a previously uncontaminated area are fundamental measures for disease eradication. In areas where the disease is already established, early pruning of any limbs or tops showing symptoms, removal of the affected organs in the partially affected crowns, and the felling of heavily infected or dead trees is recommended in order to reduce the sources of inoculum and to avoid spread of vectors to healthy trees. All the infected material, i.e. the pruned branches, and the bark removed from the stem of trees should be collected and burned.

Sanitation for single or small groups of trees is possible only if the infection is not too extensive. Only partially infected trees benefit from surgery, whereas severely damaged trees need replacing with resistant clones. The surgical removal of incipient cankers from the branches or stems of trees is followed by fungicide (benomyl or carbendazim) painting of exposed wounds and subsequent protection with a resin dressing (Marchetti and Zechini D'Aulerio, 1983). Finally, the whole tree or at least the area around the cankers is sprayed with a systemic fungicide. If necessary, these interventions should be repeated once or twice in the following years. They may save individual trees. In central Italy, 10 years of sanitation efforts in one area resulted in 5.1% diseased trees compared with 20.6% in untreated areas (Moricca and Raddi, 2000).

These sanitary measures, which are applied to ornamental trees in gardens, parks and avenues, especially in urban and peri-urban areas, may be too expensive (Puleri, 1996) or difficult to apply, and also require technical and organizational efforts, when belts of windbreaks, large plantations and cypress woods are concerned. In cypress forests and groves, the process of sanitation is to fell and remove all the affected trees quickly. Timber of felled trunks can be recovered, provided that bark and branches are removed and burned.

Cypress groves for seed production can be efficiently reclaimed by repeated and drastic application of the above sanitary measures in order to remove all the affected (and most susceptible) trees. One of the consequences of these measures is that the surviving trees not only produce relatively healthy seed, but also pollen endowed with genetic resistance to the disease, which may contribute to the improvement of seed quality.

Sanitation is currently applied in cypress nurseries and propagation plots. General information and details on the sanitation procedures, surgical and pruning methods are given in: Raddi and Panconesi, 1981a, 1981b; Nembi and Panconesi, 1982; Strouts, 1988; Parrini and Panconesi, 1991; Madar et al., 1991; Self and Chou, 1994; Vetralla et al., 1995; Panconesi and Raddi, 1998; Danti, 2001. For problems in urban and peri-urban areas, see: Graniti, 1998b, Andréoli, 1999; Moricca and Raddi, 2000.

Chemical Control

A number of protectant (dichlofluanide, chlorothalonil or, to a lesser extent, copper-based preparations) and systemic fungicides, including benomyl, carbendazim and thiophanate-methyl, better if applied as mixed or alternate treatments, have shown to be effective to control S. cardinale (Raddi and Panconesi, 1981a, 1981b, Mathon, 1982; McCain, 1984; Ponchet, 1986; Panconesi and Raddi, 1986; Moricca and Raddi, 2000).

Due to the long infection period and to the height of trees, chemical control of cypress canker by spraying trees with fungicides may be uneconomic or impractical except for nurseries or valuable ornamental plantings. Repeated applications of fungicides, from 2-3 to 4-6 times per year, particularly during the mild seasons and after pruning, are effective to prevent the disease or to stop the progress of the pathogen in recently-infected (within 10 days of infection) bark tissue. These sprays, however, have little or no value if applied as curative treatments to trees that are already diseased (Panconesi and Raddi, 1986). Problems may arise when chemical control of cypress canker is carried out in urban environment (Graniti, 1998b; Danti, 2001). Protection of pruning wounds with a systemic fungicide and with a sealing slurry or dressing is a current practice in nurseries and young plantations of ornamental trees.

Biological Control

Growth of S. cardinale was strongly inhibited by Trichoderma viride (Magro et al., 1984; Marchetti et al., 1986). The possibility of controlling infections of the pathogen with the aid of T. viride or other antagonistic microorganisms has been envisaged. Application of wet soil containing natural populations of T. viride to bark cankers favoured the healing of lesions on young cypress trees (Marchetti et al., 1986).

Host-Plant Resistance

Several investigations have shown that a high variability of susceptibility or resistance to S. cardinale exists in natural forests as well as in plantations of Cupressus sempervirens. Variability ranged from quite resistant to highly susceptible trees, indicating that resistance is a population characteristic, with mechanisms under polygenic control. Pollen is partly responsible for the resistance displayed by the progeny. Such a condition has helped breeders to select resistant clones, and to afford the genetic improvement of cypress species for resistance to the canker disease (Raddi and Panconesi, 1981a, 1981b, 1991, 1998; Xenopoulos, 1990; Raddi et al., 1998; Santini and Di Lonardo, 2000).

The most effective means of controlling cypress canker is the adoption of resistant clones, hybrids or species. Considerable efforts have been made in the past decades to develop a common strategy in the Mediterranean area (Grasso and Raddi, 1979; Raddi, 1984; Ponchet, 1986, 1990; Panconesi, 1991).

The main aims of this strategy have been: identification of cypresses resistant to the canker disease; large-scale production of selected cypress seed, which can produce a high percentage of resistant seedlings for replanting and reforestation; intraspecific crosses betweens resistant clones of cypress; interspecific crosses with resistant cypress species (Raddi and Panconesi, 1981a, 1981b, 1991; Teissier du Cros et al., 1991; Raddi and Panconesi, 1998; Santini et al., 1997b; Raddi et al., 1990, 1998). The following species of Cupressus have been considered in some breeding programmes for resistance: C. arizonica, C. bakeri, C. funebris [Chamaecyparis funebris], C. duclouxiana, C. torulosa, C. cashmeriana, C. lusitanica, C. abramsiana, C. guadalupensis, C. macnabiana, C. goveniana, C. dupreziana [C. sempervirens var. dupreziana] (Ponchet and Andréoli, 1979, 1993; Raddi et al., 2000).

Several clones of S. sempervirens have been selected (and some of them patented) for resistance to cypress canker, and are now commercially available, mostly as windbreaks or ornamentals. The same clones also exhibit rapid growth and tolerance of frost (Panconesi and Raddi, 1990, 1991a; Santini et al., 1997b). Other resistant clones, particularly adapted to urban landscape, were also selected (Andréoli, 1999).

For large-scale production of resistant clones, micropropagation and techniques for obtaining shoot cuttings were developed (Siniscalco and Pavolettoni, 1994; Capuana and Lambardi, 1995; Spanos, 1995; Spanos et al., 1997b). In nurseries, grafting vegetative propagation is generally used. Young trees (ramets) of clones of Italian cypress selected for resistance to S. cardinale, grafted onto seed-derived rootstocks, usually maintain the growth vigour and crown shape of their mother trees (ortets) if grown in the same environment (Santini et al., 1997b). Santini et al. (2000) showed that self-rooted homospecific and heteroplastic grafts did not change the resistance of the grafted clones significantly. The most suitable rootstocks are canker-resistant clones of C. sempervirens or clones of more resistant species, e.g. C. glabra, propagated by rooted cutting (Andréoli et al., 1966).

Work is now in progress to produce multiclonal varieties, i.e. mixed populations of several clones of C. sempervirens, each endowed with different resistance to S. cardinale, to be used for windbreaks or new plantations (Raddi and Panconesi, 1991; Raddi et al., 1998).

A particular problem in replanting cypress clones selected for resistance to canker disease, particularly in the urban environment, is the risk of pollinosis. Long-term breeding programmes are in progress, which include identification of clones with low and brief production of pollen, and a low content of allergens (Raddi et al., 2000).

Control of Vectors

Insects able to spread S. cardinale, such as the cypress bark borer beetles Phloeosinus aubei and the seed bug Orsillus maculatus, have to be kept under control. Removal of branches or trees killed or severely infected by S. cardinale, use of insecticide-treated trap-logs (Mendel, 1983) and insecticide sprays, especially in the nursery, are recommended. Use of pheromone traps has also been envisaged (Tiberi and Battisti, 1998).

References

Top of page

===, 1983. Report on forest research for the year ended March 1983. London, UK: HMSO, 89 pp.

Anastassiadis B, 1963. A new for Greece disease of the Cypress. Annales, Institut Phytopathologique Benaki, Athenes 5 (2), (164-6 + 3 photos). 13 refs.

Andréoli C, 1979. Comportement intérspecifique des Cupressacées vis-à-vis du Coryneum (Seiridium) cardinale Wag. In: Grasso V, Raddi P, eds. Seminario: Il Cipresso: Malattie e Difesa. Firenze, Italia: AGRIMED, Comunità Economica Europea, 195-202.

Andréoli C; Panconesi A; Ponchet J; Raddi P, 1984. Variation in virulence of Coryneum cardinale. [Variabilite du pouvoir pathogene de Coryneum cardinale. In Maladie du cypres (Coryneum cardinale). Seminar held in Florence, 20-21 October 1983.] Report, Commission of the European Communities, No. EUR 9200 EN-FR-IT, 21-28.

Andréoli C; Ponchet J, 1991. Potential use of exotic cypress species resistant to canker disease. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea, Firenze, 12-13 dicembre 1991. Firenze, Italy: CNR, Regione Toscana, CEE, 150-167.

Andréoli C; Ponchet J; Mari E, 1966. Effects du portegreffe sur la réaction du cyprès à la maladie du chancre cortical à Seiridium cardinale. Agronomie, 16:563-571.

AndrToli C, 1999. Cortical canker resistant cypress adapted to urban landscape. Acta Horticulturae, No.496:103-106; 8 ref.

Anon., 1947. Nómina de determinaciones de enfermedades de origen parasitarias efectuadas durante el año 1947 por la Sección Fitopatología del Departamento de Sanidad Vegetal, que no han sido mencionadas anteriormente para nuestro país. Agricultura Técnica, Chile, 7:236-237.

Arguedas Gamboa M, 1996. Inventory of diseases in forest species in Costa Rica. Revista Forestal Centroamericana, 5(15):20-24; 12 ref.

Ballio A; Morelli MAC; Evidente A; Graniti A; Randazzo G; Sparapano L, 1991. Seiricardine A, a phytotoxic sesquiterpene from three Seiridium species pathogenic for cypress. Phytochemistry, 30(1):131-136

Barnes I; Roux J; Wingfield MJ; Coetzee MPA; Wingfield BD, 2001. Characterization of Seiridium spp. associated with cypress canker based on -tubulin and histone sequences. Plant Disease, 85(3):317-321; 20 ref.

Barthelet J; Vinot M, 1944. Notes sur les maladies des cultures méridionales. Annales des Épiphyties, NS, 10:18-20.

Battisti A; Cantini R; Feci E; Frigimelica G; Guido M; Roques A, 2000. Detection and evaluation of seed damage of cypress, Cupressus sempervirens L., in Italy. Seed Science and Technology, 28(3):731-740; 21 ref.

Battisti A; Roques A, 1999. Efficient transmission of an introduced pathogen via an ancient insect-fungus association. Naturwissenschaften, 86(10):479-483; 23 ref.

Beresford RM; Mulholland RI, 1982. Susceptibility of farm shelter cypresses to three fungi associated with cypress canker disease. New Zealand Journal of Forestry Science, 12(1):7-13

Binazzi A; Covassi MV; Roversi PF, 1998. Ruolo di Cinara cupressi e di altri insetti fitomizi nel deperimento del cipresso nostrale. In: Roversi PF, MV Covassi, eds. Il Nostro Amico Cipresso. Atti della Giornata di studio e aggiornamento sulle avversità del Cupressus sempervirens L., Firenze, Italia, 14 Maggio 1998. Annali dell’Accademia Italiana di Scienze Forestali, 47:55-66.

Birch TTC, 1933. Gummosis diseases of Cupressus macrocarpa. Te Kura Ngahere (New Zealand Journal of Forestry), 3:108-113.

Boesewinkel HJ, 1983. New records of three fungi causing cypress canker in New Zealand, Seiridium cupressi (Guba) comb. nov. and S. cardinale on Cupressocyparis and S. unicorne on Cryptomeria and Cupressus. Transactions of British Mycological Society, 80:544-547.

Bogdan C, 1978. Coryneum cardinale Wag. Uzcronik nekrose kore I susenja grana cempresa u Jugoslaviji. Zastita Bilja, 29:365-370.

Bruck RI; Solel Z; Ben-Ze'ev IS; Zehavi A, 1990. Diseases of Italian cypress caused by Botryodiplodia theobromae Pat. European Journal of Forest Pathology, 20(6-7):392-396

Butin H; Peredo HL, 1986. Hongos parásitos en coníferas de America del sur con especial referencia a Chile. Bibliotheca Mycologica, 101:77-78.

CABI/EPPO, 2014. Seiridium cardinale. [Distribution map]. Distribution Maps of Plant Diseases, No.April. Wallingford, UK: CABI, Map 890 (Edition 2).

Caetano MF; Ramos P; Pinto-Gañhao J, 1991. The phytosanitary situation of cypress in Portugal and the new prospects. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, Italy: CNR Regione Toscana CEE, 81-88.

Caetano MFFA, 1980. Uma grave doença das Cupressaceas em Portugal. Agros, 63:5-9.

Capuana M; Lambardi M, 1995. Cutting propagation of common cypress (Cupressus sempervirens L.). New Forests, 9(2):111-122; 19 ref.

Chou CKS, 1989. Morphological and cultural variation of Seiridium spp. from cankered Cupressaceae hosts in New Zealand. European Journal of Forest Pathology, 19(7):435-445

Chou CKS, 1990. Pathogenic variation of Seiridium spp. isolated from cankered Cupressaceae hosts in New Zealand. European Journal of Forest Pathology, 20(1):32-43

Ciccarone A, 1949. M. unicornis, pathogenic agent of a severe canker of Cupressus macrocarpa (in Kenya). [Monochaetia unicornis (C. et E.) Sacc., agente patogeno di un grave cancro dei cipressi.] Ann. Sper. agr. (n.s.) 3 (489-546). 55 refs.

Clancy KJ, 1986. New or unusual diseases recorded in 1984/85. Research Report 1984-1985 Faculty of General Agriculture, University College, Dublin Dublin, Irish Republic; University College, 165-166

Covassi M, 1991. Il Ploesinus armatus Ritter, coleottero scolitide del cipresso, nuovo per l’Italia. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, Italy: CNR Regione Toscana CEE, 190-192.

Covassi M; Binazzi A, 1979. Gli insetti del Cipresso piu communi e dannosi in Itali e cenni di lotta. In: Grasso V, Raddi P, eds. Il cipresso: malattie e difesa. Firenze, Italy: CEE Agrimed, 203-216.

Covassi M; Intini M; Panconesi A, 1975. Preliminary observations on relations between Coryneum cardinale Wag. and Phloeosinus aubei Perr. in Tuscany. Redia, 56:159-166

Cunningham GH, 1950. Twenty-fourth Annual Report of the Department of Scientific and Industrial Research, New Zealand.

Cvjetkovic B; D' Ercole N; Ercole ND', 1978. A new disease of cypress in Yugoslavia caused by the widely distributed pathogen Coryneum cardinale. [Nuova malattia del cipresso in Jugoslavia - responsabile il Coryneum cardinale gia largamente diffuso in altri paesi.] Italia Agricola, 115(7-8):113-116; 2 pl. EMB; 9 ref.

Cvjetkovic B; Glavas M, 1978. Coryneum cardinale causing bark necrosis and dieback of the branches of cypress in Yugoslavia. Zastita Bilja, 29(4):365-370

Danti R, 2001. Comments on cypress canker in Tuscany with a particular reference to sanitation. Informatore Fitopatologico, 51(7/8):44-50; 18 ref.

Danti R; Rocca Gdella; El-Wahidi F, 2009. Seiridium cardinale newly reported on Cupressus sempervirens in Morocco. Plant Pathology, 58(6):1174. http://www.blackwell-synergy.com/loi/ppa

Ducrey M; Brofas G; Andréoli C; Raddi P, 1999. Genus Cupressus. In: Tessier du Cros E, ed. Il Cipresso. Manuale tecnico. Firenze, Italia: Studio Leonardo, 8-25.

Dugelay A, 1957. General observations on the frost of Feb. 1956 in the departments of Alpes-Maritimes and Var. [Observations generales sur la gelee de fevrier 1956 dans les departements des Alpes-Maritimes et du Var.] Rev. for. franc. 9 (1), (1-27).

EPPO, 1987. EPPO List of A1 and A2 Quarantine Organisms. Paris: European and Mediterranean Plant Protection Organization Publications, Ser. B, No. 90.

EPPO, 2014. PQR database. Paris, France: European and Mediterranean Plant Protection Organization. http://www.eppo.int/DATABASES/pqr/pqr.htm

Evidente A; Motta A; Sparapano L, 1993. Seiricardines B and C, phytotoxic sesquiterpenes from three species of Seiridium pathogenic for cypress. Phytochemistry, 33(1):69-78; 31 ref.

Evidente A; Sparapano L, 1994. 7-Hydroxyseiridin and 7-hydroxyisoseiridin, two new phytotoxic ,)>-butenolides from three species of Seiridium pathogenic to cypresses. Journal of Natural Products, 57(12):1720-1725

Faddoul J, 1973. Contribution à l’étude du Coryneum cardinale Wag. Morphologie, biologie, physiologie. Thèse No. 390. Toulouse, France: Université Paul Sabatier.

Farr DF; Bills GF; Chamuris GP; Rossman AY, 1989. Fungi on Plants and Plant Products in the United States. St. Paul, Minnesota, USA: APS Press, 1252 pp.

Frankie GW; Koehler CS, 1971. Studies on the biology and seasonal history of the Cypress bark moth, Laspeyresia cupressana (Lepidoptera: Olethreutidae). Canadian Entomologist, 103(7): 947-961.

Frankie GW; Parameter JR Jr, 1972. A preliminary study of the relationship between Coryneum cardinale (Fungi imperfecti) and Laspeyresia cupressana (Lepidoptera: Tortricidae). Plant Disease Reporter, 56(11):992-994; 5 ref.

Frisullo S; Bruno G; Lops F; Sparapano L, 1997. A new agent of cypress canker in Italy. Petria, 7(3):141-158; 33 ref.

Frisullo S; Graniti A, 1990. New records of Botryosphaeria and Diplodia cankers of cypress. Proceedings of the 8th Congress of Mediterranean Phytopathological Union, Agadir, Morocco. Rabat, Morocco: Actes Editions, 431-432.

Funk A, 1974. Canadian Plant Disease Survey, 54:166-168. In: Ginns JH, ed. Compendium of Plant Disease and Decay Fungi in Canada 1969-1980 (1986).

Gimenez Verdu I, 1991. Notes on cypress canker (Seiridium cardinale (Wag.) Sutt. et Gibs.). Boletin de Sanidad Vegetal, Plagas, 17(3):423-439

Graniti A, 1986. Seiridium cardinale and other cypress cankers. Bulletin OEPP, 16(3):479-486

Graniti A, 1993. Seiridium blight of cypress - another ecological disaster? Plant Disease, 77(6):544.

Graniti A, 1994. Seiridium blight of cypress tree: some problems and perspectives. In: Capretti P, Heiniger U, Stephan R, eds. Shoot and Foliage Diseases in Forest Trees. Proceedings of the Joint Meeting. IUFRO Working Parties, Vallombrosa. Firenze, Italy: Istituto di Patologia e Zoologia forestale e agraria dell’Università, 106-111.

Graniti A, 1998. Cypress canker: a pandemic in progress. Annual Review of Phytopathology, 36:91-114; 88 ref.

Graniti A, 1998. Some remarks on cypress canker, with special reference to the urban environment. Informatore Fitopatologico, 48(6):16-26; 20 ref.

Graniti A; Frisullo S, 1990. The species of Seiridium associated with canker diseases of cypress in the Mediterranean area. In: Ponchet J, ed. Agrimed research programme. Progress in EEC research on cypress diseases. Report EUR 12493 EN. Luxembourg: Commission of European Communities, 82-89.

Graniti A; Sparapano L, 1990. Phytotoxins in the Seiridium canker disease of cypress. In: Ponchet J, ed. Agrimed research programme. Progress in EEC research on cypress diseases. Report EUR 12493 en. Luxembourg: Commission of the European Communities, 90-95.

Grasso V, 1951. A new pathogen [Coryneum cardinale] of Cupressus macrocarpa in Italy. [Un nuovo agente patogeno del Cupressus macrocarpa Hartw. in Italia.] Ital. for. mont. 6 (2), (62-5 + 7 photos). 8 refs. Cf. For. Abstr. 9 (No. 2541).

Grasso V, 1952. Coniferous species susceptible and immune to C. cardinale. [Conifere suscettibili ed immuni al Coryneum cardinale Wag.] Ital. for. mont. 7 (3), (148-9 + 7 photos).

Grasso V, 1969. Coryneum cardinale attack on Cypress cones. Ital. for. mont. 24 (4), (181-3). [13 refs.].

Grasso V; Panconesi A; Raddi P, 1979. Testing for resistance to cypress canker disease in Italy. Phytopathologia Mediterranea, 18:166-171

Grasso V; Ponchet J, Grasso V (ed. ), Raddi P, 1980. History, geographic distribution and hosts of Coryneum cardinale. [Historique, distribution geographique et hotes du Coryneum cardinale Wag.] The cypress: diseases and protection.:-Il-Cipresso:-malattie-e-difesa, 119-126; 39 ref.

Grasso V; Raddi P; eds, 1979. Cypress: diseases and protection. Il Cipresso: malattie e difesa. European Economic Community, Agrimed. Florence Italy, 255 pp.

Hennon PE, 1990. Fungi on Chamaecyparis nootkatensis. Mycologia, 82(1):59-66

Hood IA; Gardner JF; Kimberley MO; Gatenby SJ; Cox JC, 2001. A survey of cypress canker disease. New Zealand Tree Grower, 22(1):38-41.

Hutton EM, 1949. Plant diseases. Notes contributed by the biological branch. Agricultural Gazette of New South Wales, 60:595-600.

Intini M; Panconesi A, 1976. Some aspects of the biology of Coryneum cardinale in Tuscany. [Alcuni aspetti della biologia del Coryneum cardinale Wag. in Toscana.] Annali, Accademia Italiana di Scienze Forestali, 25: 19-41; 6 pl.; 28 ref.

Lanier L, 1963. A desease of Cypress due to a Coryneum sp. Rev. for. franc. 15 (3), (221-7 + 4 dgms.). 9 refs.

Linde C; Kemp GHJ; Wingfield MJ, 1997. First report of Sphaeropsis canker on cypress in South Africa. European Journal of Forest Pathology, 27(3):173-177; 18 ref.

Luisi N, 1990. Ricerche su aspetti biologici ed epidemiologici di Seiridium cardinale nell’Italia meridionale, con indicazioni di lotta. Annali dell’Accademia Italiana di Scienze Forestali, 38:286-331.

Madar Z; Liphschitz N, 1989. Historical [Histological] studies of Cupressus sempervirens L. affected by Diplodia pinea f.sp. cupressi and Seiridium cardinale. IAWA Bulletin, 10(2):183-192; 34 ref.

Madar Z; Solel Z; Kimchi M, 1989. Effect of water stress in cypress on the development of cankers caused by Diplodia pinea f.sp. cupressi and Seiridium cardinale. Plant Disease, 73(6):484-486

Madar Z; Solel Z; Sztejnberg A, 1990. The effect of Diplodia pinea f. sp. cupressi and Seiridium cardinale on water flow in cypress branches. Physiological and Molecular Plant Pathology, 37:389-398.

Madar Z; Solel Z; Sztejnberg A; Kimchi M, 1991. Effect of pruning of trunk side-branches of cypress on infection by Seiridium cardinale and Diplodia pinea f. sp. cupressi. Forest Ecology and Management, 44(2-4):255-260

Magro P; Lenna PD; Marciano P, 1984. Trichoderma viride on cypress shoots and antagonistic action against Seiridium cardinale. European Journal of Forest Pathology, 14(3):165-170

Magro P; Lenna PDi; Marciano P, 1982. Cell wall-degrading enzymes produced by Seiridium cardinale, agent of cypress canker. European Journal of Forest Pathology, 12(3):150-156

Marchetti L; Zechini D’Aulerio A, 1983. Interventi chirurgici contro il cancro del cipresso con applicazione di mastici protettivi. Informatore Fitopatologico, 33:51-54.

Marchetti L; Zechini D’Aulerio A; Grassi S, 1986. Esperienze di lotta contro il cancro del cipresso con impiego di Trichoderma viride Pers. Informatore Fitopatologico, 36:43-45.

Mathon B, 1982. Decline of cypresses and thujas caused by two fungi: Seiridium (Coryneum) cardinale and Didymascella thujina. Revue Horticole, No.228:41-44

McCain AH, 1984. Cypress canker control with fungicides. Journal of Arboriculture, 10(6):212-214

McKee RJ, 1972. Annual Report on research and technical work, 1971. Belfast, Northern Ireland: Ministry of Agriculture, 137-145.

Mendel Z, 1983. Effects of pruned and unpruned trap-logs of cypress on infestation and development of two Phloeosinus species. Phytoparasitica, 11(2):83-88

Mendel Z, 1984. Life history of Phloeosinus armatus Reiter and P. aubei Perris (Coleoptera: Scolytidae) in Israel. Phytoparasitica, 12(2):89-97

Mendel Z; Golan Y; Madar Z; Solel Z, 1983. Insect pests and diseases of cypress in Israel. La-Yaaran, 33(1-4):37-41, 47

Moricca S; BOrja I; Vendramin GG; Raddi P, 2000. Differentiation of Seiridium species associated with virulent cankers on cypress in the Mediterranean region by PCR-SSCP. Plant Pathology, 49(6):774-781; 34 ref.

Moricca S; Raddi P, 1999. Use of DNA heteroduplex analysis to distinguish the Seiridium isolates causing cypress canker in the mediterranean region. Petria, 9(1/2):113-117; 10 ref.

Moricca S; Raddi P, 2000. Research efforts to control cypress canker in urban and peri-urban areas. International Symposium on Plant Health in Urban Horticulture. Braunschweig, Germany, 22-25 May 2000. Mitteilungen aus der Biologischen Bundestalt für Land- und Forstwirtschaft, Heft 370:231-235.

Moricca S; Raddi P; Torraca G, 2001. Comparison of Seiridium species causing cankers on Cypress and Eucalyptus. Proceedings of the International Conference Eucalyptus in the Mediterranean basin: perspectives and new utilization. October 15-19, 2000. Taormina, Italy. Firenze, Italy: Centro Promozione Pubblicità, 65-71.

Moriondo F, 1967. Epidemic diseases and afforestation. Ann. Accad. Ital. Sci. For. 16 (381-406 + 6 plates). [41 refs.].

Moriondo F, 1972. Cypress canker caused by Coryneum cardinale. Part 1 : Development of infection in the stem tissues. Annali, Accademia Italiana di Scienze Forestali, 21:399-426

Motta E, 1979. Presenza di spermazi in Seiridium cardinale. Annali dell’Istituto Sperimentale per la Patologia Vegetale, 5:71-75.

Motta E, 1984. Seiridium cardinale; establishment of the pathogen on seeds of Cupressaceae and possibilities of chemical control. Annali dellIstituto Sperimentale per la Patologia Vegetale Roma, 9:205-210

Motta E, 1986. Pathogenic fungi on seeds of forest trees. Bulletin OEPP, 16(3):565-569; 24 ref.

Motta E; Saponaro A, 1983. Micoflora dei semi di Cupressacee. Annali dell’Istituto Sperimentale per la Patologia Vegetale, 8 (1982-1983):71-75.

Mujica Richatt F; Vergara Castillo C, 1980. Flora Fungosa Chilena Revisada y actualizada por E. Oehrens Bertossi. Universidad de Chile, Facultad de Agronomía, Ciencias Agricolas No. 5. Santiago de Chile: Editorial Universitaria.

Mutto S; Panconesi A, 1987. Ultrastructural modifications in Cupressus sempervirens tissues invaded by Seiridium cardinale. European Journal of Forest Pathology, 17(4-5):193-204

Nag Raj TR, 1994. Coelomycetous anamorphs with appendage-bearing conidia. Waterloo, Ontario, Canada: Mycologue Publications.

Nattrass RM; Booth C; Sutton BC, 1963. Rhynchosphaeria cupressi sp. nov., the causal organism of Cupressus canker in Kenya. Trans. Brit. Mycol. Soc. 46 (1), (102-6 + 1 plate). 14 refs.D.

Nattrass RM; Ciccarone A, 1947. Monochaetia canker of Cupressus in Kenya. Emp. For. Rev. 26 (2) (289-90 + plates).

Nembi V; Panconesi A, 1982. Surgical treatment of Cupressus sempervirens attacked by Seiridium cardinale. Informatore Fitopatologico, 32(3):59-62

Neves N; Moniz F; Azevedo N de; Ferreira MC; Ferreira GWS, 1986. Present phytosanitary situation of Portuguese forests. Bulletin OEPP, 16(3):505-508

Panconesi A, 1990. Pathological disorders in the Mediterranean basin. In: Ponchet J, ed. Agrimed Research Programme. Progress in EEC Research on Cypress Diseases. Report EUR 12493 EN. Luxembourg: Commission of the European Communities, 54-81.

Panconesi A, 1991. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, 12-13 dicembre 1991. Firenze, Italy: CNR Regione Toscana CEE.

Panconesi A; Casini N; Santini A; Stefanini FM, 1995. Effect of Seiridium cardinale on growth of cypress (Cupressus sempervirens) clones. Canadian Journal of Forest Research, 25(1):109-113

Panconesi A; Ongaro L, 1982. Seiridium (Coryneum) cardinale (Wag.) Sutton & Gibson: epiphytological aspects in some Cupressus woods in Monte Morello (Florence). Rivista di Patologia Vegetale, 18(3/4):109-121

Panconesi A; Raddi P, 1986. The influence of some chemical treaments on cypress canker disease development. European Journal of Forest Pathology, 16(2):83-86

Panconesi A; Raddi P, 1990. The future for cypress: selection of clones resistant to canker. Cellulosa e Carta, 41(1):29-31; 8 ref.

Panconesi A; Raddi P, 1991. Agrimed No. 1 and Bolgheri: two new cypress varieties resistant to canker. Cellulosa e Carta, 42(1):47-52

Panconesi A; Raddi P, 1991. Cancro del cipresso. Aspetti biologici ed epidemiologici. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, Italy: CNR Regione Toscana CEE, 49-60.

Panconesi A; Raddi P, 1998. Some observations and comments on cypress canker in Tuscany. Annali - Accademia Italiana di Scienze Forestali, 47:13-34; 23 ref.

Panconesi A; Santini A; Casini N, 1993. Conservation of the germinability and pathogenicity of the conidia of Seiridium cardinale (Wag.) Sutton & Gibson, in relation to the epidemiological spread of the disease. Informatore Fitopatologico, 43(9):45-49

Panconesi A; Santini A; Casini N; degl’Innocenti C, 1994. Seiridium cardinale spread in the woody tissue of Cupressus sempervirens. In: Capretti P, Heiniger U, Stephan R, eds. Shoot and foliage diseases in forest trees. Proceedings of the Joint Meeting. IUFRO Working Parties, Vallombrosa. Firenze, Italy: Istituto di Patologia e Zoologia forestale e agraria dell’Università, 138-141.

Parrini C; Panconesi A, 1991. I metodi di lotta contro il cancro corticale del cipresso. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, 12-13 dicembre 1991. Firenze, Italy: CNR Regione Toscana CEE, 94-109.

Pivi R, 1995. Primi risultati di un’indagine epidemiologica sul cancro del cipresso in Toscana. In: Regione Toscana. Il recupero del cipresso nel paesaggio e nel giardino storico, 37-41.

Poggesi A, 1979. Intensità e ripercussioni economiche degli attacchi parassitari di Coryneum (Seiridium) cardinale Wag. e da Cinara cupressi Bckt. sul cipresso comune, con particolare riferimento alla provincia di Firenze. In: Grasso V, Raddi P, eds. Seminario: Il Cipresso: Malattie e Difesa. Firenze, 23/24 Novembre 1979. Firenze, Italy: AGRIMED, Comunità Economica Europea, 135-147.

Ponchet J(Editor), 1990. Agrimed research programme: progress in EEC research on cypress diseases. EUR Report, No. 12493:xi + 144 pp.

Ponchet J, 1986. Results of the common research programme on cortical canker of Cupressus. Bulletin OEPP, 16(3):487-498

Ponchet J; Andreoli C, 1979. Research on sources of resistance to Coryneum (Seiridium) cardinale Wag. in the genus Cupressus. Phytopathologia Mediterranea, 18:113-117

Ponchet J; Andréoli C, 1984. Host-parasite relations in the pair Cupressus-Coryneum cardinale Wag. Agronomie, 4(3):245-255

Ponchet J; Andréoli C, 1989. Histopathology of cortical canker of cypress caused by Seiridium cardinale. European Journal of Forest Pathology, 19(4):212-221

Ponchet J; Andréoli C, 1990. Compartmentalization and reactions in the host. In: Ponchet J, ed. Agrimed research programme. Progress in EEC research on cypress diseases. Report EUR 12493 EN. Luxembourg: Commission of the European Communities, 96-111.

Ponchet J; Andréoli C; Xenopoulos S; Caetano MF; Raddi P; Panconesi A, 1990. Pathogenic variability in Seiridium. In: Ponchet J, ed. Agrimed research programme. Progress in EEC research on cypress diseases. Rep. EUR 12493 EN, Luxembourg: Commission of European Communities, 112-126.

Ponchet J; AndrToli C, 1993. Cypresses and their potential. PHM Revue Horticole, No. 337:50-55.

Puleri F, 1996. Cypress canker: costs of treatment in Tuscany. Sherwood - Foreste ed Alberi Oggi, 2(2):12-16; [Number 9].

Quezel P, 1985. Definition of the Mediterranean region and the origin of its flora. In: Gomez-Campo GL, ed. Plant conservation in the Mediterranean area. The Hague, The Netherlands: Junk.

Raddi P, 1984. Maladie du cyprès (Coryneum cardinale). Communications scientifiques présentées au séminaire de Florence (Italie) 20 et 21 octobre 1983. Rapport EUR 9200 en-fr-it. Luxembourg: Commission des Communautés Européennes.

Raddi P; Moricca S; Andréoli C, 2000. Cypress pollen: botanic aspects in fourteen cypress species and prospects for research. Allergie et Immunologie, 31(3):125-127.

Raddi P; Panconesi A, 1977. Genetic improvement of Cupressus sempervirens for resistance to Coryneum cardinale. Informatore Fitopatologico, 27(1):15-19

Raddi P; Panconesi A, 1981. Cypress canker disease in Italy: biology, control possibilities and genetic improvement for resistance. European Journal of Forest Pathology, 11(5/6):340-347

Raddi P; Panconesi A, 1981. Valorizzazione del patrimonio genetico per la resistenza al cancro del cipresso. Annali dell’Accademia di Scienze forestali, 47:45-53.

Raddi P; Panconesi A, 1991. Miglioramento genetico del cipresso per la resistenza al cancro: stato attuale e prospettive. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, 12-13 dicembre 1991. Firenze, Italiy: CNR Regione Toscana CEE, 110-120.

Raddi P; Panconesi A, 1998. Genetic improvement programme of cypress for canker resistance. Annali - Accademia Italiana di Scienze Forestali, 47:45-53; 4 ref.

Raddi P; Panconesi A; Roversi PF; Covassi MV, 1998. Valorizzazione del patrimonio genetico per la resistenza al cancro del cipresso. Annali dell’Accademia Italiana di Scienze Forestali, 47:45-53.

Raddi P; Panconesi A; Sumer S, 1987. Il cipresso in Turchia: considerazioni di un viaggio di studi. Monti e Boschi, 1:67-72.

Raddi P; Panconesi A; Xenopoulos S; Ferrandes P; Andréoli C, 1990. Genetic improvement for resistance to canker disease. In: Ponchet J (ed). Progress in EEC research on cypress diseases, 127-136.

Raddi S; Santini A; Casini N, 1994. Comparison of enzymatic polymorphism in different Seiridium isolates. Proceedings of the 9th Congress of Mediterranean Phytopathological Union, Kusadasi, Turkey. Izmir: Turkish Phytopathological Society, 281-285.

Roques A; Battisti A, 1999. Insects pests of cypress. In: Tessier du Cros E (ed). Cypress. A Practical Handbook. Studio Leonardo, Firenze, 74-95.

Santini A; Camussi A; Raddi P, 1997. Genetic variability of canker resistance trait in Cupressus sempervirens L. progenies. Journal of Applied Genetics, 38(4):453-461; 9 ref.

Santini A; Casini N; Lonardo Vdi; Raddi P, 1997. Canker resistance stability of some Cupressus sempervirens clones to Seiridium cardinale. Journal of Genetics & Breeding, 51(4):269-277; 38 ref.

Santini A; Lonardo Vdi, 2000. Genetic variability of the 'bark canker resistance' character in several natural provenances of Cupressus sempervirens. Forest Pathology, 30(2):87-96; 26 ref.

Santini A; Raddi P; Panconesi A; Lonardo Vdi, 2000. Rootstock effects on the reaction of grafted cypress to Seiridium cardinale bark canker disease. Agronomie, 20(3):325-331; 14 ref.

Saponaro A; Motta E, 1981. Some observations on the presence of Seiridium cardinale (Wag.) Sutton & Gibson on Cupressus seeds. Annali dell'Istituto Sperimentale per la Patologia Vegetale Roma, 7:71-77

Saponaro A; Motta E, 1984. Seiridium cardinale and other fungus species on seeds of Cupressaceae. [Seiridium cardinale ed altre specie fungine su semi di Cupressaceae. In Maladie du cypres (Coryneum cardinale). Seminar held in Florence, 20-21 October 1983.] Report, Commission of the European Communities, No. EUR 9200 EN-FR-IT, 57-63; 12 ref.

Saravi-Cisneros R, 1953. Canker of Cypresses caused by C. cardinale in Buenos Aires province. [Cancrosis de los cipreses provocada por Coryneum cardinale Wagener en la provincia de Buenos Aires (Argentina).] Revista de la Facultade de Agronomia, Universidad Nacional de la Ciudad Eva Peron 29 (1), (107-19). 10 refs.

Schiller G; Madar Z, 1991. Variation in foliage resin composition within the Italian cypress (Cupressus sempervirens L.) species complex and its relation to canker diseases. European Journal of Forest Pathology, 21(3):179-184

Self NM; Chou CKS, 1994. Pruning effect on incidence and severity of Seiridium cypress canker in a stand of Cupressus lusitanica. New Zealand Journal of Forestry Science, 24(1):75-77

Shishkina AK; Tsanava NI, 1970. On new diseases of ornamental plants in Georgia. Mikol. i Fitopatol., 4:76-79.

Siniscalco C; Pavolettoni L, 1994. Propagation by shoot cutting of Cupressus sempervirens clones resistant to Seiridium cardinale. Monti e Boschi, 45(2):38-41; 9 ref.

Smith CO, 1938. Inoculation on conifers with the cypress Coryneum. Phytopathology, 28:760-762.

Solel Z; Madar Z; Kimchi M; Golan Y, 1987. Diplodia canker of cypress. Canadian Journal of Plant Pathology, 9(2):115-118

Solel Z; Messinger R; Golan Y; Madar Z, 1983. Coryneum canker of cypress in Israel. Plant Disease, 67(5):550-551

Spanos KA, 1995. Screening for resistance to Seiridium canker in the Cupressaceae and vegetative propagation of cypresses. PhD thesis. Aberdeen, Scotland, UK: Department of Forestry, University of Aberdeen.

Spanos KA; Pirrie A; Woodward S, 1997. In vitro expression of resistance responses to Seiridium species in micropropagated shoots of Cupressus sempervirens and Chamaecyparis lawsoniana. Canadian Journal of Botany, 75(7):1103-1109; 37 ref.

Spanos KA; Pirrie A; Woodward S, 1997. Micropropagation of Cupressus sempervirens L. and Chamaecyparis lawsoniana (A. Murr.) Par. Silvae Genetica, 46(5):291-295; 26 ref.

Spanos KA; Pirrie A; Woodward S; Xenopoulos S, 1999. Responses in the bark of Cupressus sempervirens clones artificially inoculated with Seiridium cardinale under field conditions. European Journal of Forest Pathology, 29(2):135-142; 29 ref.

Spanos KA; Woodward S, 1997. Responses of Cupressus and Chamaecyparis callus tissues to inoculations with Seiridium cardinale. European Journal of Forest Pathology, 27(1):13-21; 31 ref.

Sparapano L; Evidente A, 1995. Studies on structure-activity relationship of seiridins, phytotoxins produced by three species of Seiridium. Natural Toxins, 3:166-173.

Sparapano L; Evidente A; Ballio A; Graniti A; Randazzo G, 1986. New phytotoxic butenolides produced by Seiridium cardinale, the pathogen of cypress canker disease. Experientia, 42(6):627-628

Sparapano L; Graniti A; Evidente A, 1995. Recent progress of the research on toxins produced by species of Seiridium associated with cypress canker diseases. Shoot and foliage diseases in forest trees. Proceedings of a Joint Meeting of the IUFRO Working Parties S2.06.02 and S2.06.04, Vallombrosa, Firenze, Italy 6-11 June 1994., 126-131; 17 ref.

Sparapano L; Luisi N; Evidente A, 1995. Comparison of pathogenic and toxigenic isolates of Seiridium cardinale from cankered cypresses. Shoot and foliage diseases in forest trees. Proceedings of a Joint Meeting of the IUFRO Working Parties S2.06.02 and S2.06.04, Vallombrosa, Firenze, Italy 6-11 June 1994., 132-137; 14 ref.

Sßnchez ME; Gibbs JN, 1995. The ecology of fungal cankers on Cupressus macrocarpa in southern England. European Journal of Forest Pathology, 25(5):266-273; 9 ref.

Strouts RG, 1970. Coryneum canker of Cupressus. Plant Path. 19 (3), (149-50).

Strouts RG, 1973. Canker of Cypresses caused by Coryneum cardinale Wag. in Britain. European Journal of Forest Pathology, 3(1):13-24

Strouts RG, 1982. Coryneum canker of Monterey cypress [in the UK]. Arboriculture Research Note, Department of the Environment, UK, No. 39, 2 pp.; 1 ref.

Strouts RG, 1988. Coryneum canker of Monterey cypress and related trees. Arboriculture Research Note - Department of the Environment, UK, No. 39 (rev.):3 pp.

Sumer S, 1987. The distribution of cypress (Cupressus L.) in Turkey and the current status in its pests and diseases, especially cypress canker disease. Istanbul Universitesi Orman Fakultesi Dergisi. Seri A, 37(1):46-66

Sutton BC, 1975. Coelomycetes. V. Coryneum. Mycological Papers Commonwealth Mycological Institute. Kew, Surrey UK, No. 138:224 pp.

Sutton BC, 1980. The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Wallingford, UK: CAB International.

Sutton BC; Gibson IAS, 1972. Seiridium [Coryneum] cardinale. CMI Descr. pathogen. Fungi Bact. No. 326, 1972. pp. [2]. [1 ref.].

Swart HJ, 1973. The fungus causing Cypress canker. Transactions of the British Mycological Society, 61(1):71-82; OBD; 22 ref.

Swart WJ; Wingfield MJ; Grant WS, 1993. Comparison of Sphaeropsis sapinea and Sphaeropsis sapinea f.sp. cupressi. Mycological Research, 97(10):1253-1260

Tabata M, 1991. Distribution and host range of Seiridium unicorne in Japan. Transactions of the Mycological Society of Japan, 32:259-264.

Teissier du Cros E, 1999. Il Cipresso. Manuale tecnico. Firenze, Italia: Studio Leonardo. (Translated into English, French, Greek, Portuguese and Spanish). Publication supported by the Commission for European Communities.

Teissier du Cros E; Ferrandes P; Hallard F; Ducatillon C; Andréoli C, 1991. Cypress Genetic improvement in France. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, 12-13 dicembre 1991. Firenze, Italy: CNR Regione Toscana CEE, 121-127.

Tiberi R; Battisti A, 1998. Relationships between phytophagous insects and cypress canker. Annali - Accademia Italiana di Scienze Forestali, 47:35-44; 28 ref.

Tisserat NA; Nus A; Barnes LW, 1991. A canker disease of the Cupressaceae in Kansas and Texas caused by Seiridium unicorne. Plant Disease, 75(2):138-140

Tonon G, 1994. Preliminary survey aimed at establishing early screening methods for the Cupressus sempervirens - Seiridium cardinale pathosystem. Journal of Genetics & Breeding, 48(4):339-343

Tonon G; Capuana M; Michelozzi M, 1995. Effects of Seiridium cardinale culture filtrate on ethylene production in Cupressus sempervirens L. Journal of Genetics & Breeding, 49(2):191-193

Torres JJ, 1969. Grave enfermedad de los cipréses en España. Bolletin Serv. Plagas Forestales, 12:97-99.

Tsopelas P; Angelopoulos A; Nikolaou K, 2008. Seiridium cardinale is a new threat to cypress trees in Cyprus. Plant Pathology, 57(4):784. http://www.blackwell-synergy.com/loi/ppa

Urbasch I, 1993. Natural occurrence of Seiridium cardinale on Thuja in Germany. Journal of Phytopathology, 137(3):189-194

Urbasch I, 1994. Current status report on the distribution of the Cupressaceae canker pathogen Seiridium cardinale in Germany. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes, 46(9):189-192

Valdivieso JA; Luisi N, 1987. Cupressus canker (Seiridium cardinale (Wag.) Sutt. & Gibs.) in Chile. Fitopatologia, 22(2):79-84

Valdivieso JA; Luisi N; Bravo TJ, 1988. Susceptibility of Cupressaceae in Chile to Seiridium cardinale canker. Bosque, 9(1):9-15

Vetralla G; Santini A; Casini N, 1995. The sanitation of cypress in Tuscany. Shoot and foliage diseases in forest trees. Proceedings of a Joint Meeting of the IUFRO Working Parties S2.06.02 and S2.06.04, Vallombrosa, Firenze, Italy 6-11 June 1994., 142-145; 7 ref.

Viljoen CD; Wingfield BD; Wingfield MJ, 1993. Comparison of Seiridium isolates associated with cypress canker using sequence data. Experimental Mycology, 17(4):323-328

Wagener WW, 1928. Coryneum canker of Cypress. Science, NS, 67:584.

Wagener WW, 1939. The canker of Cupressus induced by Coryneum cardinale n. sp. Repr. from J. agric. Res. 58 (1-46).

Wagener WW, 1948. The New World cypresses. II. Diseases of American cypresses. Aliso, 1:257-321.

Wagener WW, 1964. Diseases of Cupressus. FAO-IUFRO Symposium on International Dangerous Forest Diseases and Insects. Oxford, UK: 17-24.

Werff HS van der, 1988. Cypress canker in New Zealand plantations. New Zealand Journal of Forestry Science, 18(1):101-108

Wingfield MJ; Swart WJ, 1988. Cypress canker in South Africa. Abstracts. 5th Congress of Plant Pathology, Kyoto, Japan, No. 4-3.

Wolf CB, 1939. Other species of Cypresses as substitutes for the Monterey. Calif. Citrogr. 24 (222-5). R.A.M. 18 (562-3).

Xenopoulos S, 1984. Current research on biological aspects of the cypress canker disease and on methods to find resistant clones or provenances of the common cypress in Greece. In: CEE-AGRIMED Séminaire Cyprès-Platane. Antibes, 20-30 octobre 1984.

Xenopoulos S, 1990. Screening for resistance to cypress canker disease (Seiridium cardinale) in several Greek provenances of Cupressus sempervirens. Eur. J. For. Path., 20:140-147.

Xenopoulos S; Diamandis S, 1985. A distribution map for Seiridium cardinale causing the cypress canker disease in Greece. European Journal of Forest Pathology, 15(4):223-226

Xenopoulos S; Tsopelas P, 2000. Sphaeropsis canker, a new disease of cypress in Greece. Forest Pathology, 30(3):121-126; 12 ref.

Xenopoulos SG, 1991. Pathogenic variability of various isolates of Seiridium cardinale, S. cupressi and S. unicorne inoculated on selected Cupressus clones and seedlings. European Journal of Forest Pathology, 21(3):129-135

Xenopoulos SG, 1991. The cypress health state in Greece and new prospect from current research. In: Panconesi A, ed. Il Cipresso. Proposte di valorizzazione ambientale e produttiva nei paesi mediterranei della Comunità Economica Europea. Firenze, Italy: CNR Regione Toscana CEE, 61-70.

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