- Summary of Invasiveness
- Taxonomic Tree
- Notes on Taxonomy and Nomenclature
- Diseases Table
- Distribution Table
- Risk of Introduction
- Pathogen Characteristics
- Host Animals
- Pathway Causes
- Pathway Vectors
- Vectors and Intermediate Hosts
- Economic Impact
- Environmental Impact
- Social Impact
- Risk and Impact Factors
- Distribution Maps
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IdentityTop of page
Preferred Scientific Name
- Paenibacillus larvae
Other Scientific Names
- Paenibacillus larvae subsp. larvae (White 1906)
Summary of InvasivenessTop of page
P. larvae is a rod-shaped bacterium that causes American foul brood (AFB), a destructive disease of honey bee colonies. The origin of AFB is unknown, but it is found worldwide. Bees move spores around the hive and robber bees can assist in the transmission of spores between hives. Beekeepers may also spread the disease by moving equipment from contaminated hives to healthy ones (Lindstrom et al., 2008; OIE, 2013a; Ministry for Primary Industries, 2013).
This disease is on the list of diseases notifiable to the World Organisation for Animal Health (OIE) and is a notifiable pest according to DEFRA (DEFRA, 2011; FERA, 2013). The disease and pathogen are included in the Invasive Species Compendium because of the OIE listing.
Taxonomic TreeTop of page
- Domain: Bacteria
- Phylum: Bacteroidetes
- Class: Sphingobacteria
- Order: Sphingobacteriales
- Family: Flexibacteraceae
- Genus: Paenibacillus
- Species: Paenibacillus larvae
Notes on Taxonomy and NomenclatureTop of page
Paenibacillus is a genus of Gram-positive, facultative anaerobic, endospore-forming bacteria. Members of this genus were originally included in Bacillus, until reclassification separated them into a distinct genus in 1993 (Ash et al., 1993).
Paenibacillus larvae causes American foul brood in honeybees (Apis mellifera). Previously, American foul brood and powdery scale disease were considered distinct (Graaf et al., 2006), but this is no longer valid and the pathogenic agents, Paenibacillus larvae subsp. larvae and Paenibacillus larvae subsp. pulvifaciens, were reclassified as one species, Paenibacillus larvae (Genersch et al., 2006), although the subspecies are still referred to in the literature.
DistributionTop of page
American foul brood has a worldwide distribution (D’Alessandro et al., 2007) and cases have been reported in almost all the beekeeping regions of the five continents (Antúnez et al., 2004). It appears to be uncommon or even absent in significant parts of sub-Saharan Africa (Fries and Raina, 2003), but has been reported from some countries there (Hansen et al., 2003).
Studies have shown geographical clustering of different genotypes; for example Peters et al. (2006) reported non-random distribution of five different genotypes in the district of Arnsberg, Germany.
Distribution TableTop of page
The distribution in this summary table is based on all the information available. When several references are cited, they may give conflicting information on the status. Further details may be available for individual references in the Distribution Table Details section which can be selected by going to Generate Report.Last updated: 10 Jan 2020
Risk of IntroductionTop of page
P. larvae is already present in most regions where honey bees are kept, but spread on a more local level is still a concern.
It is the spores that are the infectious agent; they can be spread from colony to colony by transfer of wax, queens, combs or honey (OIE, 2013a).
Recent studies have also shown that small hive beetles (Aethina tumida) are vectors of P. larvae and should be considered in control programmes for American foul brood where both pests are present (Schäfer et al., 2010).
Robber bees may enter a hive that has become weakened by AFB infection and take contaminated honey back to their hives, so assisting disease spread to other colonies and apiaries. Studies have shown that transmission of AFB between apiaries can occur within 1 km from clinically-diseased colonies in the absence of robber bees, but over longer distances when they are present (Lindström et al., 2008).
The movement of bees, equipment and supplies worldwide has assisted in spreading bee diseases to all areas where bees are raised (OIE, undated).
Pathogen CharacteristicsTop of page
P. larvae are Gram-positive, rod-shaped, round-ended and spore-forming bacteria. They are only visible under a high-power microscope. They vary greatly in size, being 0.5 μm wide and between 1.5 and 6 μm long; they occur both singly and in chains and filaments. Some strains are motile. Spores contaminate bee larval food and larvae ingest and become infected by the spores. The spores germinate in the larval gut and the vegetative bacteria are nourished by the larvae. Before the vegetative form of the bacterium dies, millions of spores are produced and can be spread to other bees. (OIE, 2013a; Pennsylvania Department of Agriculture, undated).
Studies have shown geographical clustering of different genotypes. Peters et al. (2006) reported five different genotypes (AB, Ab, ab, aβ and A) in Arnsberg, Germany in studies between March 2003 and October 2004. Two genotypes were found in one hive and it was reported that the five genotypes were not randomly distributed in the district.
Host AnimalsTop of page
Pathway CausesTop of page
Pathway VectorsTop of page
Vectors and Intermediate HostsTop of page
Economic ImpactTop of page
American foul brood is a serious disease of honey bees worldwide and causes considerable economic losses (Basualdo et al., 2008). This disease has caused a significant decrease in honeybee populations, beekeeping industries and agricultural production (Antúnez et al., 2010). Honey bees are important to the agricultural and horticultural sectors as pollinators -- the value of pollination is estimated to exceed the value of products from beehives many-fold (Delaplane and Mayer, 2000) -- so any disease causing decline in bee populations will have a significant impact on their role in these industries.
Environmental ImpactTop of page
Impact on Habitats
A decline in bee numbers has been attributed to American foul brood, amongst other bee diseases. Bee decline will have a significantly negative affect on pollination within habitats that rely on these insects for development (Delaplane and Mayer, 2000).
Impact on Biodiversity
A decline in native bees due to the spread of American foul brood will have a negative effect on bee biodiversity (Cuthbertson and Brown, 2009).
Social ImpactTop of page
The effect of American foul brood outbreaks on honeybee health will also have a significant impact on honey products and thus the livelihood of beekeepers.
Risk and Impact FactorsTop of page Invasiveness
- Highly mobile locally
- Has high reproductive potential
- Has propagules that can remain viable for more than one year
- Reproduces asexually
- Host damage
- Increases vulnerability to invasions
- Negatively impacts agriculture
- Negatively impacts animal health
- Negatively impacts livelihoods
- Reduced native biodiversity
- Threat to/ loss of native species
- Highly likely to be transported internationally accidentally
- Difficult to identify/detect as a commodity contaminant
- Difficult to identify/detect in the field
ReferencesTop of page
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Al-Fattah M A A, El-Awady M, Gelan M I, Barakat O S, 2010. Microbiological and molecular diagnosis of American foulbrood in honeybee (Apis mellifera L.) colonies. Arab Journal of Biotechnology. 13 (1), 1-12. http://www.acgssr.org/BioTechnology/Volume13N1January2010_files/Full_Paper/001.pdf
Alippi A M, 1992. Characterization of Bacillus larvae White, the causative agent of American foulbrood of honey-bees. First record of its occurrence in Argentina. Revista Argentina de Microbiología. 24 (2), 67-72.
CABI, Undated. CABI Compendium: Status as determined by CABI editor. Wallingford, UK: CABI
Hadžimuratović M, Nevjestić A, Rukavina L, Sabirović M, 1986. Prevalence of bee and brood diseases in Bosnia and Hercegovina in the period 1980-1984. (Stanje rasprostranjenosti bolesti pcela i legla u periodu 1980-1984 godine u Bosni i Hercegovini.). Veterinarski Glasnik. 40 (7/8), 505-508.
Hansen H, Brødsgaard C J, Kryger P, Nicolaisen M, 2003. A scientific note on the presence of Paenibacillus larvae larvae spores in sub-Saharan African honey. Apidologie. 34 (5), 471-472. DOI:10.1051/apido:2003029
Näumann G, Mahrt E, Himmelreich A, Mohring A, Frerichs H, 2012. Traces of contamination-well preserved in honey: investigation of veterinary drugs and American foulbrood in honeys of global origin. Journal für Verbraucherschutz und Lebensmittelsicherheit. 7 (1), 35-43. http://www.springerlink.com/content/x6048603j860/ DOI:10.1007/s00003-011-0750-z
OIE, 2009. World Animal Health Information Database - Version: 1.4., Paris, France: World Organisation for Animal Health. https://www.oie.int/
Schuch D M T, Tochetto L G, Sattler A, 2003. Detection of Paenibacillus larvae subsp. larvae spores in Brazil. (Isolamento de esporos de Paenibacillus larvae subsp. larvae no Brasil.). Pesquisa Agropecuária Brasileira. 38 (3), 441-444. DOI:10.1590/S0100-204X2003000300015
OrganizationsTop of page
World: IBRA, International Bee Research Association, Unit 6, Centre Court, Main Avenue, Treforest, RCT, CF37 5YR, UK, www.ibra.org.uk
World: OIE (World Organisation for Animal Health), 12, rue de Prony, 75017 Paris, France, http://www.oie.int/
UK: British Beekeepers’ Association, National Beekeeping Centre, Stoneleigh Park, Stoneleigh, Warwickshire, CV8 2LG, UK, www.britishbeekeepers.com
ContributorsTop of page
23/03/2012: Original text by:
Dr Claire Beverley, CABI, Nosworthy Way, Wallingford, Oxfordshire, OX10 8DE, UK.
Distribution MapsTop of page
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