Novel EST-SSRs for an important quarantine pest Liriomyza sativae (Diptera: Agromyzidae) and their cross-species transferability.
Liriomyza sativae is a globally distributed quarantine pest. We isolated polymorphic microsatellite loci from transcriptomic data for this species and tested their potential application in population genetics and cross-species transferability in other Agromyzidae species. Of the 130 microsatellite loci selected for validation, 40 were successfully amplified, and 18 were verified as polymorphic in L. sativae. The number of alleles (Na) ranged from two to ten, with polymorphism information content (PIC) values between 0.289 and 0.818. Observed (HO) and expected (HE) heterozygosity varied from 0.250 to 0.900 and 0.314 to 0.859, respectively. Low among-population differentiation was also indicated by pairwise FST (0.032-0.045). Structure analysis and principal coordinate analysis (PCoA) suggested three clusters for the 94 samples from three different locations (FZNT, SMSX, and QZYC) in Fujian Province, China. The low genetic differentiation and clear genetic structure among populations of this invasive species may result from extensive human activity. In additional, cross-species tests validated the amplification of 16, 19, 11 and 9 of 40 loci in Liriomyza huidobrensis, Liriomyza trifolii, Chromatomyia horticola and Ophiomyia phaseoli, respectively, showed the potential application of these markers for population genetic analysis in these species.