Teleocidin compounds from the soil-derived Streptomyces ardus WS-65090 and their bioactivities.
To investigate the secondary metabolites produced by Streptomyces ardus WS-65090 from soil and their bioactivities. Five teleocidin compounds (1-5) were isolated from the ethyl acetate extract of fermentation culture of S. ardus strain WS-65090 by preparative HPLC. The compounds were elucidated as new teleocidin analogue 14-O-acetylteleocidin B-2 (1), and new naturally occurring teleocidin analogue 14-O-acetylteleocidin B-3 (2), and three known compounds teleocidin B-2,B-3 and A-2 (3-5) by extensive spectroscopic analysis, including HR-MS,1D and 2D NMR, combined with chemical transformation methods and comparison with literature data,etc. Insecticidal activities and cytotoxicity of compounds 1-5 were investigated under laboratory conditions. All compounds showed insecticidal activities against Spodoptera frugiperda,Helicoverpa armigera,Plutella xylostella, and Aphis craccivora with LC50 values from 36.5 to 305.7 μg/mL, and exhibited cytotoxicity against A875,HepG2 and Marc-145 cell lines with IC50 from 3.08 to 18.40 μM.