Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Genetic diversity of Metarhizium anisopliae isolated of insects and agroecosystems.

Abstract

Background: The genus Metarhizium is integrated of various species, they have a wide geographic distribution and are considered one of the best biological control agents for pests insect. Objective: To determine the genetic diversity of Metarhizium strains isolated from different insects and agroecosystems using RAPD markers. Methodology: Soil was collected from different agroecosystems (Mexican lemon, grapefruit, sugar cane, mango), insects Diatraea saccharalis (Lepidoptera: Crambidae), Diatraea magnifactella (Lepidoptera: Crambidae), Schistocerca piceifrons piceifrons (Orthoptera: Acridylus), Macrodactylus murinus (Coleoptera: Scarabaeidae), Spodoptera frugiperda (Lepidoptera: Noctuidae) and the mite Boophilus microplus (Acari: Ixodidae) to isolate Metarhizium strains, the isolates were analyzed through random amplified polymorphic DNA (RAPD) markers. Results: The RAPD markers were highly informative and polymorphic. The generated dendrogram was made up of four clusters (A-D); cluster A consisted of 11 soil isolates, one from D. magnifactella and two from D. saccharalis. In cluster B one of D. saccharalis and one of soil. On the other hand, in cluster C were that B. microplus and one of soil. While cluster D was integrated by the S. frugiperda, M. murinus and S. piceifrons isolates. Implications. The determination of the genetic diversity between Metarhizium isolates obtained from insects and agroecosystems, generates a contribution on the habitat that will allow the development of strategies to carry out an adequate biological control of pest insects. Conclusion: The analyzes using the RAPD markers indicated that there is a genetic diversity among the Metarhizium isolates collected from different insects and agroecosystems.