Invasive Species Compendium

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Abstract Full Text

Cloning and expression profile analysis of four pheromone-binding protein genes in the fall armyworm, Spodoptera frugiperda.


The fall armyworm, Spodoptera frugiperda, is a major agricultural pest wordwide. This pest has invaded and rapidly spread in more than 20 provinces in China by January 2019. Use Of pheromone lures is an effective method for population monitoring and mass-trapping of S. frugiperda. However, the mechanism of seX pheromone reception is not yet Clear in this insect species, limiting the development and application of pheromone lures With high efficiency. Pheromone-binding proteins (PBPS) play critical roles in the recognition of intraspeeifie seX pheromones in most lepidopteran species, including S. frugiperda. In this study, four CDNA sequences encoding PBPs were Cloned from S. frugiperda and named as SfruPBPI - SfruPBP4. The four SfruPBPs all contained complete open reading frames, and the proteins encoded by these genes all have the remarkable features of PBPs; The N-terminal signal peptides and siX positionally conserved cysteine residues. Phylogenetic analysis result showed that the four SfruPBPs were Closely related to PBPs from Spodaptera litura and S. littoralis, and that the four SfruPBPs fell into different branches. The four SfruPBPs all consisted of three exons and two introns, with highly conserved intron insertion sites. Moreover, the four SfruPBP genes were tandemly arrayed in the genome of S. frugiperda. SfruPBPI, SfruPBPZ and SfruPBP3 were specifically expressed in the antennae of adults; of these, the transcription levels of SfruPBP] and SfruPBPZ in male antennae were significantly higher than those in female antennae, while the variation in SfruPBP3 transcription between male and female antennae was not significant. SfruPBP4 was specifically expressed in the abdomen of male adults. This study pave the way for revealing the mechanism underlying pheromonal recognition in S. frugiperda.