Rapid species-specific PCR identification of Bactrocera tau (Diptera:Tephritidae).
Objective: A rapid method to detect Bactrocera tau(Walker) was established for inspection on produce to prevent potential introduction and spread of the invasive pest. Method: Based on the species-specific PCR(SS-PCR)technology, B. tau as control and 20 common fruit flies of similar morphology were used to extract the genomic DNA templates, and the homology of the specimens checked with the COI mt DNA sequence. A rapid and accurate identification method applying the B. tau-specific primers NF404 and NR610 was designed and verified by PCR amplification and electrophoresis analysis. Result: The newly developed SS-PCR detection method clearly amplified a single 207 bp band only on the target B. tau, not on any other fruit flies. Comparisons on the test results on the insects or body parts of all fruit flies with homogenous band sequence to B. tau validated the new methodology. Conclusion: The newly established SS-PCR method exhibited high repeatability, specificity, and accuracy in detecting B. tau. It was considered applicable for the entry/exit and quarantine inspection on the invasive insect, larvae or dead body parts found on vegetables and fruits.