Creation and identification of Cucumis sativus-C. hystrix alien addition lines.

Abstract

Alien addition lines are important interspecific materials, which can be used for introgression of interspecific genetic material and gene mapping. Creating a variety of alien addition lines plays an important role in cultivar improvement and related research of cucumber. In this experiment, based on the allotetraploid Cucumis hytivus from the cross of C. sativus and C. hystrix, two backcrosses with diploid cultivar C. sativus were carried out to obtain 22 plants of BC₂. The plant containing alien chromosome in BC₂ population was identified by GISH technique. Further, oligo-FISH technology and 12 C. hystrix chromosome specific single-copy sequence markers were used to accurately identify the alien chromosomes. Finally, a total of four different alien additional lines were screened, including three monosomic alien addition lines (MAALs) which contained C. hystrix chromosomes H06, H10, and H12, respectively, and one double alien monosomic addition line containing H06 and H10.