Invasive Species Compendium

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Abstract

Spatiotemporal expression of CYP4G15 and CYP4L4 and their response to high and low temperature stress in Spodoptera frugiperda.

Abstract

Objectives: To explore whether CYP4G15 and CYP4L4 are involved in the response to high and low temperature stress in Spodoptera frugiperda. Methods: The expression of CYP4G15 and CYP4L4 were detected by RT-PCR at 36°C and 4°C in different developmental stages and tissues. Results: Expression of both genes was highest in young larvae. Expression of CYP4G15 was highest in the epidermis, whereas that of CYP4L4 was highest in the foregut. The two genes were differentially expressed at high and low temperatures. Conclusion: CYP4G15 and CYP4L4 may be involved in the synthesis of various endogenous substances in S. frugiperda, as well as metabolic processes under high and low temperature stress.