DNA barcoding-based assessment of the invasive and native non-crustose Codium species in the central Cantabrian sea, southern Bay of Biscay.
In this work, we identified non-crustose invasive (Codium fragile subsp. fragile) and native Codium spp. (Codium tomentosum and Codium vermilara) in the central Cantabrian Sea using DNA barcoding (tufA and rbcL genes). We designed a new FCO tuf A genetic marker for identifying Codium spp. in fresh and herbarium material. The tufA and rbcL sequences revealed three different single haplotypes for each of the species and a lack of intraspecific genetic diversity. The FCO tuf A genetic marker revealed one new haplotype of C. fragile within the native region (South Korea), suggesting the possibility of higher genetic diversity in the donor region of this invasive species.