Screening of phytochemicals and allelopathic potential of selected botanicals on germination and growth of maize (Zea mays L.) at Paklihawa, Nepal.
Phytochemical screening of six different botanicals (Azadirachta indica Juss, Artemisia dubia Wall, Lantana camara L, Parthenium hysterophorus L, Justicia adhatoda Linn. and Moringa oleifera L.) and their allelopathic potential on germination and growth of maize was done at Agriecology lab of Institute of Agriculture and Animal Science, Paklihawa Campus, Rupandehi. Among the qualitative tests done for the presence of secondary metabolites, saponin was found to present in all the botanicals extract except in M. oleifera L. Most of the tested metabolites were extracted by the aqueous solution rather than other 3 menstrums (acetone, methanol and petroleum ether). Similarly, Azadirachta indica Juss and Artemisia dubia Wall were found to contain most of the tested metabolites as compared to other botanicals tested. Result of quantitative test revealed that percentage of alkaloid was found 11.58%, 6.78%, 18.01%, 17.75%, 8.20% & 17.78% and flavonoid 12.89%, 2.85%, 7.5%, 15.32%, 11.36%, 13.63% respectively in M. oleifera L., A. dubia Wall., L. camara L., J. adhatoda Linn., A. indica Juss and P. hysterophorus L. Allelopathic effect of those botanicals extract on radical and plumule growth of maize was examined invitro condition for 15 days. Seven treatments (leaf extract of A. indica, A. dubia, L. camara, P. hysterophorus. J. adhatoda and M. oleifera and distilled water as control) maintained as 3 different concentrations (stock solution, 10% of stock solution & 25% of stock solution) were arranged in CRD design with 3 replications. Application of highest concentration (stock solution) of P. hysterophorus extract found to cause the maximum reduction in radical length of maize seedlings. Similarly, mild concentration of L. camara (25% stock solution) caused maximum reduction in plumule length. Thus, Lantana camara and P. hysterophorus found to possess allelopathic effect on growth of maize seedlings and probably.