Invasive Species Compendium

Detailed coverage of invasive species threatening livelihoods and the environment worldwide

Abstract

Phylogenetic analysis of the mitochondrial genomes invading giant African snail Achatina fulica in China.

Abstract

Objective: To analyze the population genetic structure of the invasive Species based on mitochondrial genome sequence analysis of the samples of Achatina fulica (A. fulica) collected from multiple locations in China. Methods: A total of 99 samp1es of A. fulica collected from 18 sampling sites in Guangxi Zhuang Autonomous Region, Guangdong province, Guizhou province, Yunnan province, Hainan province and Fujian province were obtained. The mitochondrial genome assembly sequence was obtained by Illumina Hiseq sequencing which used to phylogenetic analysis by maximum likelihood method. Results: The total length of the mitochondrial genome sequence obtained by sequencing assembly is 15 382-17 125 hp, including 13 protein coding gene sequences, 21 tRNA sequences and two ribosomes sequence (12 S and 16 S). The mitochondrial genome alignment of the 80 samples showed a high degree of sequence similarity (100%). The remaining 19 samples have only small differences in sequence similarity. The results of the evolution analysis of the differential sample showed a certain geographical clustering, but the- bootstrap value was low. Conclusion: The mitochondrial genome is not suitable for the genetic structure analysis of the genetic p0pulation Structure of A. fulica currently invading China.