Invasive Species Compendium

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Abstract

Effect of petroleum ether extract from Solidago canadensis on liver of Pomacea canaliculata.

Abstract

Objective: On the basis of previous studies, the effect of petroleum ether extract of Solidago canadensis (PEEE) on the liver of Pomacea canaliculata was discussed in depth to provide a basis for further effective control the harm of P. canaliculata to agricultural production and realizing the resource utilization of the malignant alien invasive plant S. canadensis. Method: Chlorinated tap water was used as the control group, and PEEE suspensions of 0.11, 0.18 and 0.29 mg?mL -1 were used as the experimental groups. In each group, 10 adult P. canaliculata of the same size and vigor were randomly placed, and the beaker was sealed with a layer of gauze and rubber band to prevent the P. canaliculata from escaping. At room temperature (23-27°C), the treatment was carried out for 48 h with 3 repetitions in each group. After 48 h, the P. canaliculata treated with dechlorinated tap water, 0.11, 0.18 and 0.29 mg?mL -1 were randomly selected, the shells of P. canaliculata were broken and the liver was taken out, and the ultrastructure of liver tissue was observed by scanning electron microscopy and transmission electron microscopy. The liver tissue of P. canaliculata treated with 0.29 mg?mL -1 was lyophilized for metabonomics study. Result: The results of scanning electron microscopy showed that the liver surface of P. canaliculata was damaged after soaking for 48 h with different concentrations of PEEE, and the degree of damage was positively related to the concentration of PEEE treatment solution. High concentrations of PEEE could cause liver atrophy and loss of elasticity, wrinkle bulge erosion, body surface damage, flocs and loose texture. The results of transmission electron microscopy showed that the liver cells of P. canaliculata were damaged obviously after soaking for 48 h with different concentrations of PEEE. The number of rough endoplasmic reticulum and mitochondria in liver cells of P. canaliculata decreased with high concentrations of PEEE, and the rough endoplasmic reticulum appeared swelling, breaking and degranulation. Mitochondria showed swelling and irregular shape. The results of metabonomic experiments showed that after PEEE treatment, the content of 60 metabolites in the liver of P. canaliculata changed, and several metabolic pathways were affected. The two pathways with the highest enrichment were glycosphingolipid metabolism and palmitoyl-CoA entering mitochondrial matrix pathway. Conclusion: PEEE of S. canadensis has great damage and destruction to the liver cells of P. canaliculata, so it has strong molluscicidal activity and is expected to be developed into a new natural molluscicidal drug with great potential development and application value.