Invasive Species Compendium

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Abstract Full Text

First report of Curvularia lunata causing postharvest fruit rot of banana in Pakistan.


Banana (Musa acuminata Colla) fruits infected with rot disease were collected from the local market of Lahore, Pakistan. The observed symptoms were dry, decayed, sunken lesions with dark grey to greenish in color, damaging quality of the fruit. The pathogen was isolated on malt extract agar plates and observed under light microscope and scanning electron microscope for morphological characterization. The young fungal colony was greenish grey in color which upon maturation turned into downy dark grey to black. The conidia were single or in chains, attached with brown geniculate conidiophores distinguish by septation with dark brown scars. The mature conidia were curved at subterminal ends, dark brown to reddish brown in color with 3 oblique septa. On the basis of these morphological characters, the fungal isolate was identified as Curvularia lunata (Wakker) Boedijn. Pathogenicity test was performed by inoculating the pathogen artificially on asymptomatic banana and the re-isolated fungus had the same characters as that of originally isolated one. For more accuracy, the molecular studies of the isolated pathogen C. lunata were performed by sequencing its rDNA with two distinct markers ITS and GAPDH. The PCR products of ITS and GAPDH were then submitted to GenBank with MN752153 and MN787829 accession numbers, respectively. To the best of our knowledge, C. lunata infecting the banana fruit has not already been reported from Pakistan. Hence, this is the first report of C. lunata responsible for post-harvest banana rot in Pakistan.