Invasive Species Compendium

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Species and antibiotic sensitivity of culturable gut bacteria of Spodoptera frugiperda larvae from Mangshi, Yunnan.

Abstract

Objective: To determine the species of culturable bacteria in the intestine of larvae of Spodoptera frugiperda in Mangshi in Yunnan and the sensitivity of dominant bacteria to antibiotics, and provide reference for biocontrol of S. frugiperda. Method: Bacteria in the intestine of the 5th instar larvae of S. frugiperda in the corn field of Mangshi was isolated and cultured using routine isolation and culture method. The species were identified using the morphological characterisitics and 16S rDNA molecular techniques. The sensitivity of dominant bacteria to 10 antibiotics was determined using the paper diffusion method. Result: The culturable bacteria in the intestine of the 5th instar larvae of S. frugiperda in the corn field of Mangshi belonged to 3 phyla, 5 families, 5 genera and 6 species, they were Klebsiella variicola, Enterococcus mundtii, E. gallinarum, Providencia vermicola, Chryseomicrobium aureum and Kocuria himachalensis. Among them, K. variicola was the dominant bacteria species. The sensitivity of K. variicola to 10 antibiotics demonstrated that K. variicola showed a low resistance to norfloxacin, ciprofloxacin, cotrimoxazole, chloramphenicol and cefazolin, moderate resistance to gentamicin and amikacin, and high resistance to penicillin, erythromycin and ampicillin. Conclusion: The composition of culturable bacteria in the intestinal tract of larvae of S. frugiperda in Mangshi, Yunnan shows diversity, K. variicola is the dominant bacteria species, it shows an evident resistant to penicillin, erythromycin and ampicillin.